How to Optimize Buffer Components for Ni-Agarose Beads
Ni²⁺–agarose beads (typically Ni-NTA/Ni-IDA) capture recombinant proteins via coordination between multiple immobilized chelation sites and the imidazole rings of His tags.
Topic
Reducing agents
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
9 resources · protocols, FAQs & articles
Common Reducing Agents Used in Protein Experiments
Common Reducing Agents Used in Protein Experiments
Formic Acid
Formic acid can serve as both an oxidant and a reducing agent.
1-Hydrosilatrane
1-Hydrosilatrane, depicted in Figure 1, serves as a highly versatile hydride reagent. It can be employed in various reduction reactions to synthesize alcohols, amines, their chiral derivatives, and esters from aldehydes and ketones.
Aladdin®Inorganic ligands
In the interdisciplinary realm where biochemistry and inorganic chemistry converge, inorganic ligands play an essential role. These ligands bind with metal ions within living organisms to form coordination compounds that are crucial in a variety of biological processes.
Surfactant-Free Nuclear Protein Extraction Protocol
In the process of nuclear protein extraction, the use of surfactants can significantly interfere with protein labeling efficiency. This is because surfactants can alter the conformation of proteins and may interact with the target protein, affecting its biological activity and the accuracy of ...
Can it be used in non-denaturing electrophoresis?
Can it be used in non-denaturing electrophoresis?
sec-Butanol, 2-Butanol
Butan-2-ol, or sec-butanol, is an organic compound with formula CH3CH(OH)CH2CH3. Its structural isomers are 1-butanol, isobutanol, and tert-butanol. 2-Butanol is chiral and thus can be obtained as either of two stereoisomers designated as (R)-(−)-butan-2-ol and (S)-(+)-butan-2-ol. It is ...
Hypophosphorous acid and hypophosphite salts
Hypophosphorous acid and hypophosphite salts
Related topics
Techniques often explored alongside immunological experiments.