グレード＆純度Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.0.6 mg/mL
Rabbit anti Human Grp75 Antibody, Polyclonal antibody, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
Protein Function
Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone.
仕様
Product Name
Grp75 Antibody - 一次抗体, specific to Grp75, Rabbit IgG
同義語
75 kDa glucose regulated protein antibody|75 kDa glucose-regulated protein antibody|CSA antibody|Glucose Regulated Protein antibody|Grp 75 antibody|GRP-75 antibody|GRP75 antibody|Heat shock 70 kDa protein 9 antibody|Heat shock 70kD protein 9 antibody|heat shock 70kDa protein 9 antibody|Heat shock 70kDa protein 9B antibody|Heat shock protein A antibody|Hsc74 antibody|Hsp74 antibody|Hsp74a antibody|HSPA9 antibody|Hspa9a antibody|HSPA9B antibody|MGC4500 antibody|mitochondrial antibody|Mortalin 2 antibody|Mortalin antibody|Mortalin perinuclear antibody|Mortalin2 antibody|MOT 2 antibody|MOT antibody|MOT2 antibody|Mthsp70 antibody|P66 MOT antibody|PBP74 antibody|Peptide-binding protein 74 antibody|Stress-70 protein antibody|75 kDa glucose-regulated protein|CSA|GRP75|GRP-75|GRP75mthsp75|Heat shock 70 kDa protein 9|heat shock 70kD protein 9B|heat shock 70kDa protein 9 (mortalin)|heat shock 70kDa protein 9B (mortalin-2)|HSPA9|HSPA9B|HSPA9BMGC4500|Mortalin|mortalin, perinuclear|Mortalin-2|MOT|MOT2|mot-2|p66-mortalin|PBP74|Peptide-binding protein 74|stress-70 protein, mitochondrial|SAAN|CRP40|EVPLS|SIDBA4
仕様と純度
ExactAb™, Validated, Carrier Free, 0.6 mg/mL
宿主種
ウサギ
特異性
Grp75
免疫原
A synthetic peptide derived from human HSPA9 (AA 114-157).
ポジティブコントロール
WB: A549, MCF-7, HEK293, HeLa, Jurkat, NIH/3T3 and C6 cell lysates. IHC: Human brain tissue. IF/ICC: HeLa cells.
活用
Unconjugated
等級
Carrier Free, ExactAb™, Validated
アイソタイプ
Rabbit IgG
SDS-PAGE
150 kDa
精製方法
Protein A purified
集中
0.6 mg/mL
Product Line
生物製品
保管と配送
形状
Liquid
保管条件
Store at -20°C,Avoid repeated freezing and thawing
入荷
Ice chest + Ice pads
安定性と保管
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
画像
Grp75 Antibody (Ab106524) - Western Blot All lanes: Grp75 Antibody (Ab106524) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 75 kDa Observed band size: 74 kDa Exposure time: 4.5 s
Grp75 Antibody (Ab106524) - IHC Immunohistochemistry analysis of paraffin-embedded Human brain using Grp75 Antibody (Ab106524). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Grp75 Antibody (Ab106524) - IF/ICC
IF analysis of Grp75 Antibody (Ab106524) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Grp75 Antibody (Ab106524) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
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レビュー
顧客レビュー
よくある質問
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Carrier Free mean?
Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Grp75 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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