PCNA Mouse mAb - 一次抗体, specific to PCNA, IgG2a

Cat. No.: Ab181179
注文可能
グレード&純度 Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. 0.5 mg/mL
Application
Flow Cytometry, WB
Host species
マウス
Reactive species
マウス, 人間, 助言
Isotype
Mouse IgG2a
Clone Number
C12/17A3
Conjugation
Unconjugated
★
サイズ
USA
ドイツ (EU)*
価格
個数
10μl
Ab181179-10μl
在庫あり ≥10
—
$45.90
50μl
Ab181179-50μl
1 在庫あり
—
$199.90
100μl
Ab181179-100μl
1 在庫あり
—
$299.90
1ml
Ab181179-1ml
US製・受注生産 · 2~4週間 ·
—
$1,499.90
Enter a quantity for the sizes you want to add.
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このグレードを選ぶ理由

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,0.5 mg/mL Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated ベースライン干渉を最小限に抑える必要がある、感度の高いクロマトグラフィーおよび分析ワークフロー向け。

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保管・配送

Store at -20°C,Avoid repeated freezing and thawing Ice chest + Ice pads に出荷。正確な仕様については、ロット固有の COA をご確認ください。

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品質書類

SDS、COA、データシート、仕様書をダウンロードできます。ロット固有の COA はロット番号検索で入手可能です。

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文献的裏付け

クロマトグラフィー、有機合成、クロスカップリング反応に関する 0 件の査読済み論文で引用されています。

概要

Application
WB: 1/1,000-1/10,000 ; Predicted molecular weight: 29 kDa
Flow: 0.032 μg/mL - 1.0 μg/mL
Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication. Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix. Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA. PCNA protein expression is a well-accepted marker of proliferation.

仕様

Product Name
PCNA Mouse mAb - 一次抗体, specific to PCNA, IgG2a
同義語
Cyclin | PCNA
仕様と純度
Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,0.5 mg/mL
宿主種
マウス
特異性
PCNA
免疫原
Full length recombinant human PCNA
ポジティブコントロール
WB: HeLa, A431, HEK293, HepG2, NIH3T3 and C6 whole cell lysates Flow: HeLa cells
活用
Unconjugated
等級
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
アイソタイプ
IgG2a
ライトチェーン・タイプ
kappa
SDS-PAGE
185 kDa
精製方法
Protein A purified
集中
0.5 mg/mL
Product Line
生物製品
保管と配送
形状
Liquid
保管条件
Store at -20°C,Avoid repeated freezing and thawing
入荷
Ice chest + Ice pads
安定性と保管
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
画像

PCNA Mouse mAb (Ab181179) - Western Blot
All lanes: PCNA Antibody (Ab181179) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 29.0 kDa
Observed band size: 31.0 kDa
Exposure time: 54.2 s 

PCNA Mouse mAb (Ab181179) - Flow Cytometry
Overlay histogram showing HeLa cells stained with PCNA Mouse mAb (Ab181179) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).

適用#テキヨウ#
適用#テキヨウ#
Dilution info
WB

1/1,000-1/10,000 ; Predicted molecular weight: 29 kDa.

Flow Cytometry

0.032 μg/mL - 1.0 μg/mL

ドキュメント

📋 安全データシート(SDS)

危険性、取扱い、保管、および規制遵守を網羅した包括的な文書。

<<<<<<< HEAD SDS をダウンロード → ======= SDS をダウンロード → >>>>>>> release248-p3

✅ 分析証明書(COA)

ロット固有の品質データ。ロット番号を入力して、正確な COA を取得してください。

COA を検索 →

📊 データシート

製品仕様と用途のクイックリファレンス概要。

データシートを見る →

🔬 仕様書

このグレードの完全な品質特性と合格基準。

仕様書を見る →

詳細データ

関連ターゲット(人間)
PCNA Tchem Proliferating cell nuclear antigen (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
作用メカニズム
証明書(CoA、COO、BSE/TSEと分析図)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate Type日付商品
ZJ24F0102680ZJ24F0102680Certificate of AnalysisDec 16, 2025 Ab181179
ZJ24F0102681ZJ24F0102681Certificate of AnalysisDec 16, 2025 Ab181179
ZJ24F0102682ZJ24F0102682Certificate of AnalysisDec 16, 2025 Ab181179
よくある質問と記事
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Carrier Free グレードガイドを見る → Validated グレードガイドを見る → ExactAb™ グレードガイドを見る → Azide Free グレードガイドを見る → High Performance グレードガイドを見る → PBS Only グレードガイドを見る →

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