DDT Mouse mAb: FAQs for Research Use

DDT Mouse mAb is a mouse-derived monoclonal antibody specifically targeting the DDT (D-dopachrome tautomerase) protein, designed to support a range of standard immunological research applications.

Product SKU

Ab378262

Antibody Type

Mouse Monoclonal Antibody (mAb)

Target Protein

DDT (D-dopachrome tautomerase)

Host Species

Mouse

Reference Catalog

genuinbiotech#61878

What Is DDT Mouse mAb?

DDT Mouse mAb (SKU: Ab378262) is a mouse monoclonal antibody raised against the DDT protein, also known as D-dopachrome tautomerase. DDT is a member of the macrophage migration inhibitory factor (MIF) superfamily and shares structural homology with MIF. It is implicated in immune regulation and inflammatory processes. This monoclonal antibody provides high specificity for the DDT antigen, making it a valuable tool for researchers investigating DDT expression, localization, and function in biological samples.

Recommended Applications and Usage Guidelines

DDT Mouse mAb is suitable for use in standard immunological detection techniques. Typical applications for monoclonal antibodies of this class include Western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA), subject to empirical validation in your specific experimental system. Users are strongly advised to consult the product datasheet (genuinbiotech#61878) for validated application conditions, recommended dilutions, and compatible secondary antibodies before initiating experiments.

Optimization of antibody concentration and incubation conditions is recommended for each application. Appropriate positive and negative controls should always be included to confirm specificity.

Storage, Handling, and Stability

Proper storage is essential for maintaining antibody activity and specificity. As a general guideline for mouse monoclonal antibodies, DDT Mouse mAb should be stored at the temperature specified on the product datasheet, typically -20°C for long-term storage. Repeated freeze-thaw cycles should be avoided, as they can lead to protein aggregation and loss of binding activity. Aliquoting the antibody into single-use volumes upon first thaw is recommended to preserve reagent integrity.

Always consult the specific storage conditions stated on the product datasheet (genuinbiotech#61878), as formulation-specific requirements may differ from general guidelines.

Ordering and Supply Information

DDT Mouse mAb is available from Aladdin Scientific under SKU Ab378262. This product is referenced under the supplier catalog number genuinbiotech#61878. To place an order, researchers and procurement teams may contact Aladdin Scientific directly through the official website, or submit a purchase order referencing the SKU Ab378262. For bulk quantities, custom formats, or institutional pricing inquiries, please reach out to the Aladdin Scientific sales team.

Product availability and lead times may vary. It is recommended to confirm current stock levels prior to planning time-sensitive experiments.

Troubleshooting Common Issues

Researchers occasionally encounter challenges when working with monoclonal antibodies. Common issues include high background signal, weak or absent signal, and non-specific bands. To address high background, ensure thorough washing steps, optimize blocking conditions (e.g., BSA or non-fat milk concentration), and confirm that secondary antibody dilutions are appropriate. For weak signal, consider increasing primary antibody concentration, extending incubation time, or verifying that the target protein is expressed in the chosen sample type.

If no signal is observed, confirm that the sample preparation method preserves the epitope recognized by DDT Mouse mAb. For denatured antigen-dependent applications such as WB, ensure complete denaturation of the sample. Always refer to the product datasheet and contact Aladdin Scientific technical support if issues persist.

Frequently asked questions

What is the target of DDT Mouse mAb (Ab378262)?

DDT Mouse mAb targets the DDT protein, also known as D-dopachrome tautomerase, a member of the MIF (macrophage migration inhibitory factor) cytokine superfamily involved in immune and inflammatory signaling.

What applications is DDT Mouse mAb validated for?

Specific validated applications are detailed in the product datasheet referenced under genuinbiotech#61878. Common applications for antibodies of this type include Western blot, immunohistochemistry, immunofluorescence, and ELISA, but users should confirm validated conditions from the datasheet before experimental use.

How should DDT Mouse mAb be stored to maintain activity?

DDT Mouse mAb should be stored according to the conditions specified on the product datasheet. In general, mouse monoclonal antibodies are stored at -20°C for long-term use. Avoid repeated freeze-thaw cycles by aliquoting the antibody upon first thaw.

How can I order DDT Mouse mAb from Aladdin Scientific?

You can order DDT Mouse mAb using SKU Ab378262 through the Aladdin Scientific website or by contacting the sales team directly. Reference the supplier catalog number genuinbiotech#61878 for cross-referencing purposes.

What should I do if I observe high background or no signal with DDT Mouse mAb?

For high background, optimize blocking conditions and washing steps, and verify secondary antibody dilutions. If no signal is detected, confirm target protein expression in your sample, check sample preparation methods to ensure epitope preservation, and review recommended antibody concentrations in the datasheet. Contact Aladdin Scientific technical support for further assistance.

References

1. genuinbiotech Product Catalog Entry #61878: DDT Mouse Monoclonal Antibody. Genuinbiotech.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.