Recombinant Human tPA Protein: FAQs & Technical Guide

Recombinant Human Tissue-Type Plasminogen Activator/tPA Protein is a full-sequence recombinant form of the key serine protease responsible for initiating fibrinolysis by converting plasminogen to plasmin, making it an essential research tool for studies in thrombosis, hemostasis, and cardiovascular biology.

Product Name

Recombinant Human Tissue-Type Plasminogen Activator/tPA Protein

SKU

rp330095

Sequence Coverage

Full sequence (full-length)

Protein Family

Serine protease / Plasminogen activator

Species

Human (Homo sapiens)

Reference Source

Gxene Biotech #848

What Is Recombinant Human tPA Protein?

Tissue-Type Plasminogen Activator (tPA) is a serine protease encoded by the PLAT gene in humans. It plays a central role in the fibrinolytic system by catalyzing the conversion of the zymogen plasminogen into the active protease plasmin, which subsequently degrades fibrin clots. Endogenous tPA is primarily secreted by vascular endothelial cells and is tightly regulated by its principal inhibitor, plasminogen activator inhibitor-1 (PAI-1).

The Recombinant Human Tissue-Type Plasminogen Activator/tPA Protein (SKU: rp330095) is produced as a full-sequence recombinant protein, corresponding to the complete human tPA sequence. This full-length coverage ensures the presence of all functional domains — including the fibronectin finger domain, epidermal growth factor (EGF)-like domain, two kringle domains, and the catalytic serine protease domain — which are critical for fibrin binding, plasminogen interaction, and enzymatic activity.

Key Applications in Research

Recombinant Human tPA Protein is widely used across multiple research disciplines. Common applications include:

• Fibrinolysis and thrombolysis assays: Used to study clot dissolution mechanisms in vitro, including plasminogen activation kinetics and fibrin degradation.
• Enzyme activity assays: Substrate-based assays (e.g., chromogenic or fluorogenic plasminogen substrates) to measure tPA catalytic activity and screen inhibitors or activators.
• Protein–protein interaction studies: Investigating tPA binding to fibrin, plasminogen, PAI-1, and cell-surface receptors such as annexin A2.
• Cardiovascular and stroke research: Modeling thromboembolic events and evaluating thrombolytic strategies relevant to ischemic stroke treatment.
• Cell-based assays: Examining tPA-mediated signaling, receptor binding, and cellular responses in endothelial or neuronal cell models.

Because the protein covers the full sequence, it is particularly suited for structural studies and assays that require all functional domains to be intact.

Storage, Handling, and Reconstitution Guidelines

Proper storage and handling are essential to maintaining the activity and integrity of recombinant tPA protein. The following general guidelines apply; always refer to the specific Certificate of Analysis (CoA) provided with your lot for confirmed conditions:

• Storage temperature: Recombinant proteins of this class are typically stored at −20 °C for short-term storage or −80 °C for long-term preservation. Avoid repeated freeze–thaw cycles, as these can lead to aggregation and loss of enzymatic activity.
• Reconstitution: Lyophilized preparations should be reconstituted in the diluent recommended in the product datasheet (commonly sterile water, PBS, or a carrier protein solution such as 0.1% BSA in PBS). Allow the vial to equilibrate to room temperature before opening.
• Working aliquots: After reconstitution, prepare single-use aliquots to minimize freeze–thaw cycling. Aliquots can typically be stored at −20 °C or −80 °C.
• Handling: Work on ice whenever possible. Use low-binding tubes to minimize protein adsorption to tube walls.

Note: Specific storage buffers, concentration, and formulation details are provided on the product datasheet and CoA for SKU rp330095. Contact Aladdin Scientific technical support for lot-specific information.

Quality, Purity, and Regulatory Considerations

The Recombinant Human tPA Protein (rp330095) is supplied as a research-grade reagent intended for in vitro research use only and is not approved for diagnostic or therapeutic applications in humans. The product is referenced against Gxene Biotech #848 and is manufactured to cover the full human tPA sequence.

Quality attributes typically assessed for recombinant proteins in this category include:

• Purity: Assessed by SDS-PAGE and/or HPLC. Researchers should consult the CoA for lot-specific purity data.
• Biological activity: Confirmed through functional assays such as plasminogen activation or chromogenic substrate cleavage assays. Activity specifications are provided on the CoA.
• Endotoxin levels: Critical for cell-based assays; endotoxin content is measured (typically by LAL assay) and reported on the CoA.
• Identity: Confirmed by methods such as Western blot or mass spectrometry.

For full lot-specific quality data, request the Certificate of Analysis from Aladdin Scientific at the time of ordering or after receipt of your shipment.

Ordering and Support Information

Recombinant Human Tissue-Type Plasminogen Activator/tPA Protein is available from Aladdin Scientific under SKU rp330095. To place an order or request a quote, visit the Aladdin Scientific website or contact your regional sales representative.

Technical support resources available to customers include:

• Product datasheets and Certificates of Analysis (CoA)
• Technical application notes
• Pre-sales and post-sales technical support from Aladdin Scientific scientists

For questions about shipping conditions, returns, or payment options, please refer to the Help and Customer Service section of the Aladdin Scientific website, or contact customer service directly. All products are shipped under conditions designed to maintain protein integrity; refer to the shipping documentation included with your order for details.

Frequently asked questions

What is the sequence coverage of Recombinant Human tPA Protein (rp330095)?

The Recombinant Human Tissue-Type Plasminogen Activator/tPA Protein (rp330095) covers the full sequence of human tPA, meaning all functional domains of the native protein are represented in the recombinant construct. This full-length coverage is important for assays that depend on domain–domain interactions or intact fibrin-binding regions.

Is Recombinant Human tPA Protein (rp330095) suitable for cell-based functional assays?

Yes, recombinant tPA proteins with full-sequence coverage are commonly used in cell-based assays, including studies of endothelial cell responses, receptor-mediated tPA signaling, and tPA-induced plasminogen activation on cell surfaces. For cell-based work, it is important to verify endotoxin levels from the Certificate of Analysis, as elevated endotoxin can confound results. Consult the CoA for rp330095 for lot-specific endotoxin data.

How should I reconstitute and store the recombinant tPA protein to preserve its activity?

For lyophilized preparations, reconstitute according to the instructions on the product datasheet using the recommended diluent (typically sterile water or PBS, often supplemented with a carrier protein such as 0.1% BSA). After reconstitution, aliquot the protein into single-use volumes and store at −80 °C to maximize stability. Avoid repeated freeze–thaw cycles. Always allow vials to equilibrate to room temperature before opening, and work on ice during handling.

What reference material was used for this recombinant tPA product?

The Recombinant Human tPA Protein (rp330095) is referenced against Gxene Biotech #848. Researchers who require cross-comparability with other tPA reference standards should consult the product datasheet or contact Aladdin Scientific technical support for additional characterization data.

Can this tPA protein be used for inhibitor screening assays?

Yes, recombinant tPA proteins are routinely used in inhibitor screening assays, typically employing chromogenic or fluorogenic plasminogen substrates to measure tPA-mediated plasmin generation in the presence or absence of test compounds. The full-sequence coverage of rp330095 makes it appropriate for such functional assays. Confirm specific activity data from the CoA before designing your assay format.

References

1. Gxene Biotech Product Reference #848 — Recombinant Human Tissue-Type Plasminogen Activator. Internal reference cited in Aladdin Scientific product documentation.
2. Rijken DC, Sakharov DV. Basic principles in thrombolysis: regulatory role of plasminogen. Thromb Res. 2001;103 Suppl 1:S41-9.
3. Cesarman-Maus G, Hajjar KA. Molecular mechanisms of fibrinolysis. Br J Haematol. 2005;129(3):307-21.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.