Rh blood group cross-matching test
Rh blood group cross-matching test
To check whether the blood of the recipient or donor contains antigens and antibodies that are incompatible with the Rh blood group, this experiment is derived from the clinical experiment guide of the undergraduate 5-year program of Mudanjiang Medical College.
Operation method
Rh blood group cross-matching test
Principle
Papain destroys the surface charged sialic acid of erythrocytes, thus lowering the surface charge of erythrocytes and allowing them to lean, and incomplete antibodies with specificity can agglutinate with enzyme-treated erythrocytes with the corresponding antigens.
Materials and Instruments
Anti-D Incomplete Antibody Serum Papain Liquid Move 1, take four small clean test tubes were labeled "1" "2" ten control "" a control ", For more product details, please visit Aladdin Scientific website.
2、'1'Tube add 1 drop each of recipient's serum and donor's 5% erythrocyte suspension, "2'-Tube add 1 drop each of donor's serum and recipient's 5% erythrocyte suspension, "＋" tube with 1 drop each of 5% D erythrocyte saline suspension and incomplete anti-D serum, and "1" tube with 1 drop each of 5% D erythrocyte saline suspension and normal human AB serum.
3. Add 1 drop of 1% papain to each tube.
4, the tube into the 37 ℃ water bath for 2min removed, gently rotate the test tube to observe the results.
5, the experimental results of judgment. "Ten" control tube should appear agglutination. "A" control tube should not appear agglutination, such as "1" tube and "2" tube are not agglutination can be Huxiao.