Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
Avertin is an injectable laboratory animal anesthetic that uses 2,2,2-tribromoethanol (tribromoethanol, TBE) as the active ingredient. It is commonly used to induce short-duration general anesthesia in rodents such as mice.
β-Glucan is a class of polysaccharides formed by glucose residues linked through β-glycosidic bonds. It is widely present in oats, barley, yeast, fungi, algae and some microbial-derived materials. β-Glucans from different sources vary greatly in linkage type, branching degree, molecular ...
Mitochondrial isolation is a fundamental step in metabolic analysis, apoptosis research, drug toxicity evaluation, and studies of mitochondrial nucleic acids and proteomics.
Vitamin C content determination is not merely a matter of quantification, but rather a comprehensive analytical issue involving definition of the target analyte form, sample stabilization procedures, methodological selection, and the interpretive boundaries of analytical results. Because vitamin ...
Intracellular nucleic acids are not static carriers of genetic information, but rather dynamic molecular systems that continuously undergo synthesis, processing, modification, transport, recognition, degradation, and reutilization.
Alginate is a natural anionic polysaccharide mainly derived from the cell walls of brown algae. Its repeating structural units consist of β-D-mannuronic acid (M) and α-L-guluronic acid (G) linked by 1→4 glycosidic bonds, and it occurs as blockwise distributions of M-blocks, G-blocks, and ...
Diphenylamine (DPA) is a representative aromatic amine compound consisting of two phenyl rings linked to an amino group. Its aromatic amine condensation reactivity and ability to extend π-conjugation make it a key component in a variety of colorimetric systems in analytical chemistry. The ...
Gonadotropins are key endocrine effectors of the hypothalamic–pituitary–gonadal (HPG) axis and mainly include follicle-stimulating hormone (FSH) and luteinizing hormone (LH), as well as the placental hormone human chorionic gonadotropin (hCG), which exerts LH-like activity.
The readout intensity in real-time quantitative fluorescence PCR reflects not only the target amplification process, but also superimposed system-level factors such as well-to-well optical variation, pipetting volume deviations, evaporation and condensation, bubble-induced scattering, and drift ...
Cocoa and cocoa-derived products constitute a typical natural multi-component system. Their compositional profile is mainly defined by flavanol polyphenols (represented by (−)-epicatechin and proanthocyanidins), methylxanthine alkaloids (predominantly theobromine, with caffeine as a secondary ...
Calcium gluconate is the calcium salt of D-gluconic acid and is commonly available in anhydrous and monohydrate forms. Owing to its characteristic compatibility and solubility behavior in aqueous systems, calcium gluconate is widely used in medicine, occupational-safety emergency response, food ...
Paeoniflorin is a representative monoterpene glycoside enriched in the roots of Paeonia plants and is commonly encountered in medicinal or research materials such as Chishao, Baishao, Mudan, and Zimudan. Its molecular structure contains multiple hydroxyl groups and glycosidic linkages, ...
Lecithin generally refers to a class of phospholipid mixtures enriched in phosphatidylcholine(PC). It is widely distributed in plant and animal tissues and serves as a fundamental structural component of biological membranes.
Thrombin is a central serine protease in the coagulation cascade. It not only drives fibrin clot formation and promotes platelet activation, but—under specific cofactor conditions—also mediates anticoagulant pathways and cell signaling, thereby exhibiting marked pleiotropy in physiological ...
Soil health assessment is evolving from reliance on single physicochemical indices toward an integrated, multi-dimensional characterization of “microbial–biochemical–nutrient” processes, enabling precision agricultural management, evaluation of ecological restoration outcomes, and ...
Techniques often explored alongside immunological experiments.
