Mitochondrial complex I Activity Assay Kit (Micro Method)

Cat. No.: M1515822

GRADE & PURITY BioReagent

Visão geral

Mitochondrial respiratory chain complex I, also known as NADH-CoQ reductase or NADH dehydrogenase, is widely present in the mitochondria of animals, plants, microorganisms, and cultured cells. It is the largest protein complex in the inner mitochondrial membrane. This enzyme catalyzes the transfer of a pair of electrons from NADH to CoQ, while simultaneously reducing O₂ to generate O₂•⁻ (superoxide anion). It represents the primary step in the mitochondrial respiratory electron transport chain responsible for O₂•⁻ production. The activity level of this enzyme not only reflects the status of the respiratory electron transport chain but also indicates the state of reactive oxygen species generation. The detection principle of this kit is based on the ability of mitochondrial respiratory chain complex I to catalyze the dehydrogenation of NADH, generating NAD⁺. By measuring the change in absorbance of NADH at 340 nm, the activity of mitochondrial respiratory chain complex I in the sample can be calculated.

Specifications

Especificações e pureza

BioReagent

Grau

BioReagent

Aplicação

Enzyme activity assay

Tipo de amostra

Tecidos animais, Tecidos vegetais

Método de deteção

Colorimetric Method

Detection instrument

Microplate reader

Detection wavelength

340nm

Armazenamento e envio

Condições de armazenamento de armazenamento

Protected from light,Store at -20°C

Enviado em

Ice chest + Ice pads

Estabilidade e armazenamento

Store at -20℃ for 6 months. Store in the dark.

Perguntas frequentes

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at ?20 °C, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.