MAb de ratinho PD1/CD279 - Anticorpo primário, specific to PD1/CD279, Mouse IgG1

Cat. No.: Ab120764

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Blocking, Flow Cytometry, Functional Assay, Neutralization

Host species

Rato

Reactive species

Humano

Isotype

Mouse IgG1

Clone Number

J116

Conjugation

Unconjugated

Visão geral

O anticorpo anti-PD1/CD279 humano de ratinho, monoclonal (J116), pode ser utilizado para Flow, neutralização in vitro de PD-1, bloqueio in vivo de PD-1 em ratinhos humanizados e assim por diante.
Aplicação:
Fluxo: Utilizar numa concentração dependente do ensaio.
neutralização in vitro de PD-1: Utilizar numa concentração dependente do ensaio.
bloqueio in vivo de PD-1 em ratinhos humanizados: Utilizar numa concentração dependente do ensaio.
Função da proteína
Possível indutor de morte celular, em associação com outros factores.

Specifications

Product Name

MAb de ratinho PD1/CD279 - Anticorpo primário, specific to PD1/CD279, Mouse IgG1

Sinónimos

hSLE1 | antigénio CD279 | CD279 | hPD-1 | PD1 | PD-1 | PD1hPD-l | PDCD1 | morte celular programada 1 | proteína 1 de morte celular programada | Proteína PD-1 | SLEB2 | anticorpo CD279 | hPD-l | anticorpo antigénio CD279 | anticorpo hPD 1 | anticorpo hPD l

Especificações e pureza

Sem transportador, ExactAb™, sem azida, validado, ≥95% (SDS-PAGE), ver COA

Espécie hospedeira

Rato

Especificidade

PD1/CD279

Controlo positivo

IHC-P: Human tonsil tissue.

Conjugação

Unconjugated

Grau

Azide Free, Carrier Free, ExactAb™, PBS Only, Validated

Isótipo

Mouse IgG1

Tipo de corrente de luz

kappa

SDS-PAGE

150 kDa

Método de purificação

Protein G purified

Concentração

See COA

Armazenamento e envio

Forma

Líquido

Condições de armazenamento de armazenamento

Conservar a 2-8°C. Evitar a congelação e descongelação repetidas

Enviado em

Gelo húmido

Estabilidade e armazenamento

Expedido a 4°C. Conservar a 4°C durante um longo período (12 meses). Não congelar. Após a receção, recomenda-se a aliquotagem.

Imagens

Flow Cytometry: PD1/CD279 Mouse mAb (Ab120764)

PD1/CD279 Mouse mAb (Ab120764) - Flow Cytometry
Flow Cytometry analysis of unstimulated (left) or 500 ng/mL Ionomycin (I139530) and 10 ng/mL PMA (P408905) co-stimulated (right) Jurkat cells labelling PD1/CD279 (red) with PD1/CD279 Mouse mAb (Ab120764) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicação

• Aplicação: Dilution info
• Flow Cytometry:

  Use at an assay dependent concentration.

• in vitro PD-1 neutralization:

  Use at an assay dependent concentration.;

• in vivo PD-1 blockade in humanized mice:

  Use at an assay dependent concentration.;

Perguntas frequentes

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at 2–8 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (PD1/CD279 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.