PD1 Mouse mAb (APC) - Anticorpo primário, specific to PD1, Mouse IgG1

Cat. No.: Ab178780

GRADE & PURITY Validated, ExactAb™, Ex:650nm, Em:660nm, 5 μL/test

Application

Flow Cytometry

Host species

Rato

Reactive species

Humano

Isotype

Mouse IgG1

Clone Number

EH12.2H7

Conjugation

APC

Visão geral

Application:
Flow: 5 μL/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

Specifications

Product Name

PD1 Mouse mAb (APC) - Anticorpo primário, specific to PD1, Mouse IgG1

Sinónimos

CD279 | hPD-1 | PD1 | PD-1 | PD1hPD-l | PDCD1 | programmed cell death 1 | programmed cell death protein 1 | Protein PD-1 | SLEB2 | CD279 antibody | CD279 antigen antibody | hPD 1 antibody | CD279 antigen | hPD l antibody | hPD-1 antibody | hSLE1 antibody

Especificações e pureza

ExactAb™, Validated, Ex:650nm, Em:660nm, 5 μL/test

Espécie hospedeira

Rato

Especificidade

PD1

Imunogénio

Recombinant protein of human CD279

Controlo positivo

Flow: PMA and lonomycin stimulated (overnight) MOLT4

Conjugação

APC

Excitação (nm)

Red Laser(633nm)

Emissão (nm)

660nm

Ex/Em(nm)

Ex:650nm;Em:660nm

Grau

Em:660nm, Ex:650nm, ExactAb™, Validated

Isótipo

Mouse IgG1

Tipo de corrente de luz

kappa

Método de purificação

Protein G purified

Concentração

5 μL/test

Armazenamento e envio

Condições de armazenamento de armazenamento

Store at 2-8°C,Protected from light,Do not freeze

Enviado em

Wet ice,Do not freeze

Estabilidade e armazenamento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imagens

Flow Cytometry: PD1 Mouse mAb (APC) (Ab178780)

PD1 Mouse mAb (APC) (Ab178780) - Flow Cytometry
MOLT-4 cells treated with 500 ng/mL Ionomycin and 10 ng/mL Phorbol-12-myristate-13-acetate (PMA) for 24 hours (right panel) are stained with PD1 Mouse mAb (APC) (Ab178780) (red) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG Isotype Control Antibody (APC) (Ab180309). Black - Unlabelled control, cells without incubation with primary antibody. Untreated MOLT-4 cells were used as control (left panel).

Aplicação

• Aplicação: Dilution info
• Flow Cytometry:

  5μl/test

  For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

Alvos associados (humanos)

PDCD1 Tclin Programmed cell death protein 1 (0 Activities)

Discover Target: PDCD1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

PDCD1 Tclin Programmed cell death protein 1 (14 Activities)

Discover Target: PDCD1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mecanismos de ação

Perguntas frequentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (PD1 Mouse mAb (APC)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.