AC-262536 - 10mM in DMSO , CAS No.870888-46-3

CAS: 870888-46-3 Cat. No.: A655766 Formula: C₁₈H₁₈N₂O Molecular Weight: 278.35 PubChem CID: 44512434

GRADE & PURITY 10mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Overview

AC-262536 is a selective and non-steroidal androgen receptor modulators ( SARMs ) with beneficial anabolic effects. AC-262536 exhibits potent agonist activity at the androgen receptor , with an affinity in the low nanomolar range (1-10 nM).

In Vitro

AC-262536 dose-dependently inhibits dihydroxytestosterone (DHT)-induced proliferation of LNCaP. The effects are significant with 100 nM AC-262536 (%inhibition 47.2±12.2), and reaches a plateau with 1 μM treatment (%inhibition 50.7±7.6). Thus, AC-262536 can act as a functional antagonist in prostate cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

AC-262536 (3, 10, 30 mg/kg) reverses the luteinizing hormone (LH) spike in castrated rats . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Male Sprague-Dawley rats (200-225 g) Dosage: 3, 10 and 30 mg/kg Administration: Administered subcutaneously; once daily for 14 consecutive days Result: Significantly decreased LH levels by about 40% at the 3 mg/kg dose. The ED 50 was determined to be 2.8 mg/kg. At the 10 and 30 mg/kg doses, the effects of AC-262536 were significantly stronger than Testosterone Propionate (TP) : 1.91±0.32 ng/mL and 1.53±0.34 ng/mL vs. 3.12±0.69 ng/mL, respectively.

Specifications

Specifications & Purity

10mM in DMSO

Biochemical and Physiological Mechanisms

AC-262536 is a selective and non-steroidal androgen receptor modulators ( SARMs ) with beneficial anabolic effects. AC-262536 exhibits potent agonist activity at the androgen receptor , with an affinity in the low nanomolar range (1-10 nM).

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

This product requires cold chain shipping. Ground and other economy services are not available.

Action Type

AGONIST

Names and Identifiers

• Isomeric SMILES: C1C[C@H]2CC(C[C@@H]1N2C3=CC=C(C4=CC=CC=C43)C#N)O
• PubChem CID: 44512434
• Molecular Weight: 278.35

Application Protocols

Not specified for this item; refer to CoA/Spec Sheet.

General discovery-chemistry protocol guidance (non-item-specific):

• Stock preparation: Dissolve in anhydrous DMSO to a defined concentration (e.g., 10–50 mM). Vortex/sonicate briefly; avoid prolonged heating. Record lot number, concentration, and date.
• Storage of stocks: Aliquot into low-bind tubes, exclude air (argon or nitrogen overlay if feasible), and store at the recommended temperature. Avoid repeated freeze–thaw.
• Cell-based assays: Titrate a concentration range (e.g., 10-point half-log dilutions) keeping final DMSO constant across wells. Include vehicle controls.
• Data quality: Confirm stability by re-analyzing remaining working solutions via HPLC/LC–MS. Monitor for precipitation or adsorption losses.

These are general practices; adapt to your assay and institutional SOPs.

Biological Roles

Item-specific biological targets or mechanistic annotations are not provided in the Product Data. The following context is general and should not be taken as a characterization of this specific lot; consult primary literature and the CoA/SDS.

General context for nonsteroidal receptor-directed small molecules:

• Such compounds are often designed to modulate nuclear receptor signaling in vitro, enabling study of receptor-dependent transcriptional programs, coactivator recruitment, and downstream gene expression.
• They can serve as chemical tools for dissecting pathways related to protein–ligand interactions, receptor selectivity, and structure–activity relationships (SAR).
• In cell biology, these molecules are frequently used to interrogate dose–response, time-dependence, and reversibility of receptor-mediated effects, while controlling for vehicle impacts and off-targets.

Experimental considerations:

• Verify compound activity in your assay context with positive/negative controls and orthogonal readouts (e.g., reporter assays vs. endogenous target gene changes by qPCR).
• Consider species differences in receptor sequences and cofactor expression when comparing data across human vs. rodent cell lines.
• Assess potential cytotoxicity independently (e.g., ATP or resazurin viability assays) to deconvolute on-target signaling from non-specific effects.

Compliance note: This product is provided strictly for laboratory research use. No medical, diagnostic, or clinical applications are supported.

Buffer Applications

This product is a bioactive small molecule rather than a buffering agent. Classic buffer systems (phosphate, HEPES, Tris, etc.) are not applicable for pH control with this item.

Practical guidance for assay media (general):

• Prepare concentrated stocks in DMSO and dilute into standard biological buffers (e.g., HBSS, PBS) or culture media (e.g., DMEM, RPMI) immediately before use.
• Maintain consistent final DMSO across treatments (commonly ≤0.1–0.5% v/v), as buffer capacity and cell health can be impacted by solvent.
• If precipitation occurs upon dilution, increase dilution steps, add a small cosolvent fraction (e.g., ethanol), or include a minimal amount of surfactant (e.g., 0.01% Tween-20) compatible with your assay.
• Filter stocks (0.22 µm PTFE/PES) when sterility is required; avoid membrane materials incompatible with organic solvents.

Buffer recipes, pH ranges, and ionic strength are dictated by the biological system, not this compound; use established formulations for your assay and verify compound stability therein.

Green Alternatives

As a bioactive small molecule used in discovery research, the main environmental impact stems from solvent use and waste management rather than the compound itself. Item-specific greener alternatives to the molecule are not applicable; however, greener handling strategies can be adopted.

Greener practice options (general):

• Prefer DMSO–water systems over halogenated or high-toxicity solvents when feasible.
• Minimize solvent volumes via microplate-based assays and automated low-dead-volume liquid handling.
• Employ ethanol as a secondary solvent instead of DMF where solubility permits.
• Use solvent recycling for chromatographic purification if you synthesize analogs in-house.
• Choose aqueous-compatible assay formats (fluorescence polarization, TR-FRET) that operate at low compound concentrations to reduce solvent load.

Illustrative comparison (general; not item-specific):

• DMF vs DMSO: DMSO typically offers lower worker exposure concerns and better biodegradability profile than DMF, though both require controlled disposal.
• Halogenated solvents (e.g., DCM): Avoid for routine stock handling; reserve only for necessary analytical work, and segregate waste streams.

Waste considerations:

• Collect DMSO- or ethanol-containing assay waste as hazardous. Avoid drain disposal. Follow institutional and local regulations.

Pharmaceutical Uses

No pharmacopeial status, excipient role, or formulation designation is provided in the Product Data. This product is labeled For research use only and is not intended for human or veterinary use.

General R&D formulation context (non-clinical, discovery stage):

• For in vitro studies, DMSO stock solutions are commonly prepared and diluted into aqueous media. Adsorption to plastics and serum-binding should be assessed experimentally.
• For non-clinical exploratory work (e.g., mechanistic animal studies within approved institutional protocols), typical vehicles include mixtures of PEG400, ethanol, Kolliphor/cremophor, and saline; selection should be driven by solubility/stability and ethical committee approvals. Item-specific in vivo guidance is not provided here.
• Stability-indicating methods (HPLC/UPLC with photodiode array and MS) are recommended to track degradation under storage and dosing conditions.

Compliance note: Aladdin Scientific supplies this compound strictly for laboratory research. No therapeutic claims, dosing recommendations, or clinical applications are provided or implied.

Physical Properties

Item-specific physicochemical parameters are not provided in the Product Data. Use the CoA/SDS for authoritative specifications.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Melting point (MP): Not specified for this item; refer to CoA/Spec Sheet.
• Boiling point (BP): Not applicable/rarely reported for bioactive solids; Not specified for this item; refer to CoA/Spec Sheet.
• Density: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Not specified for this item; refer to CoA/Spec Sheet.
• LogP/logD: Not specified for this item; refer to CoA/Spec Sheet.
• pKa: Not specified for this item; refer to CoA/Spec Sheet.
• Refractive index: Not applicable for solids; Not specified for this item; refer to CoA/Spec Sheet.

General literature/practice (not item-specific):

• Many nonsteroidal small molecules used in receptor research are moderately lipophilic and are commonly prepared as concentrated stock solutions in anhydrous DMSO (e.g., 10–50 mM), then diluted into aqueous media with final DMSO ≤0.1–0.5% v/v for cellular work. Verify solubility and stability for this specific lot.
• Stability can be compound- and lot-dependent; minimize light/oxygen exposure and repeated freeze–thaw. Filter sterilization (0.22 µm, PTFE) is often employed for assay stocks when compatible.

Quality & Grades

• Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.

Interpretation and guidance:

• When Grade/Purity is not listed, rely on the lot-specific CoA for purity (e.g., HPLC area %), identity (LC–MS and/or NMR), and residual solvents. Documented analytical data are essential for quantitative biology.
• If an “assay (HPLC)” value is provided on the CoA, it reflects chromatographic purity under defined conditions; spectral confirmation (NMR/MS) supports identity but does not replace purity profiling.
• Low-UV-absorbing excipients or stabilizers, if present, should be declared on the CoA. None are specified here.
• For screening and SAR work, consider additional in-house QC as needed: orthogonal HPLC methods (different stationary phases), HRMS for exact mass, and water content by Karl Fischer if hygroscopicity is suspected.
• If using in bioassays, verify endotoxin level if cell responses could be confounded; not typically specified for small-molecule reagents unless intended for specialized workflows.

Reaction & Applications

This product is supplied as a research-use small molecule, not principally as a synthetic reagent. No manufacturer reaction/application notes are provided for chemical synthesis.

• Not typically used as a stoichiometric reagent or catalyst in organic synthesis.
• If derivatization or conjugation is desired (e.g., for probe development), consult the structure on the CoA/SDS to identify modifiable positions and functional handles. Absent item-specific structural data here, do not assume availability of reactive groups.

Assay-oriented applications (general guidance):

• Prepare concentrated DMSO stocks (e.g., 10–50 mM) and perform serial dilutions to generate dose–response curves in biochemical or cellular assays.
• Include vehicle controls matched for DMSO percentage, and verify compound stability over the assay period (e.g., re-inject by HPLC after incubation).
• Adsorption/adhesion: Some hydrophobic small molecules adsorb to plastics; low-bind tubes/plates or inclusion of carrier proteins (e.g., 0.1% BSA) can mitigate, if compatible with the biology.

Reaction Conditions

Not applicable. This product is not supplied as a synthetic reagent, and no manufacturer reaction condition guidance is provided.

If adapting for chemical modification (general, non-item-specific):

• Solvent systems: Anhydrous polar aprotic solvents (DMSO, DMF, NMP) or mixed systems may be required depending on functional groups; confirm structural compatibility from CoA/SDS.
• Temperatures: Many functionalized bioactives are sensitive to heat; keep modifications at low to moderate temperatures and monitor by LC–MS.
• Atmosphere: Use inert gas (N2/Ar) if air- or moisture-sensitive motifs are present (unknown here).
• Workup/purification: Employ reversed-phase or normal-phase chromatography as dictated by polarity; track with MS-enabled detection due to UV overlap with impurities.

Because item-specific reactivity and stability are not disclosed in the Product Data, do not undertake chemical modifications without first obtaining full structural and analytical information.

Safety & Handling

Product Data do not specify GHS hazard details for this item. Always consult the SDS for authoritative safety information.

• Signal word / H-statements / Pictograms / GHS classification: Not specified for this item; refer to SDS.
• Intended use: For research use only (Product Data). Not for human or veterinary use.

General laboratory safety (good practice):

• PPE: Use lab coat, safety glasses, and appropriate chemically resistant gloves. Handle powders in a fume hood or ventilated enclosure to avoid inhalation of dust/aerosol.
• Incompatibilities: Until SDS review, avoid strong oxidizers, strong acids/bases, and conditions leading to thermal decomposition. Keep away from ignition sources when using organic solvents (e.g., DMSO, DMF).
• First aid (overview; defer to SDS): If inhaled, move to fresh air; if on skin, wash with water/soap; if in eyes, rinse cautiously with water for several minutes; if ingested, seek medical attention. Provide SDS to responders.
• Environmental precautions: Prevent release to the environment. Dispose of contents/container according to institutional hazardous waste procedures.
• Special risks for bioactive small molecules: Even without GHS classification, minimize exposure (use closed handling, avoid skin contact/ingestion) due to potential biological activity at low doses.

Solvent Selection

Item-specific solubility data are not provided. The following are general practices for hydrophobic small-molecule research compounds; verify empirically for your lot.

• Primary stock solvent (general): Anhydrous DMSO is commonly used due to broad solvency and biological compatibility at ≤0.1–0.5% v/v final concentration in cells.
• Alternative solvents (trial, literature practice): DMF, ethanol (absolute), or 1:1 co-solvent mixtures (DMSO:PEG400, DMSO:EtOH) followed by dilution into aqueous buffers with surfactants (e.g., 0.01–0.1% Tween-20) if needed.
• Aqueous compatibility: Direct aqueous dissolution is often limited for nonsteroidal bioactives; titrate in small volumes of cosolvent while monitoring for precipitation upon dilution.
• pH effects: If functional groups are titratable, solubility can be pH-dependent; absent item-specific pKa data, avoid extreme pH to reduce degradation risk.
• Practical tips:
  • Warm gently (room temp to 37°C) and vortex/sonicate to assist dissolution; avoid prolonged heating.
  • Filter sterilize DMSO stocks with PTFE or PES membranes; avoid cellulose esters if solvent-incompatible.
  • Record the maximum soluble concentration in your assay medium (visible precipitation can confound data).

Comparison (general):

• DMSO vs EtOH: DMSO typically affords higher solubility and lower volatility; ethanol offers easier removal but may solubilize less and can affect membranes at ≥0.5–1% v/v.

Storage & Reconstitution

• Storage conditions (Product Data): Store at -80°C.
• Shipping (Product Data): Dry ice packs + Cold packs to maintain low temperature during transit.
• Appearance: Not specified for this item; refer to CoA/Spec Sheet.

Reconstitution (general guidance; verify with CoA):

• Solvent: Start with anhydrous DMSO to prepare a concentrated stock (e.g., 10–50 mM), adjusting based on observed solubility. If needed, consider co-solvents (DMF, ethanol) compatible with intended use.
• Technique: Allow vial to equilibrate to room temperature in a desiccated environment before opening to prevent condensation. Weigh quickly, dissolve with gentle vortexing/sonication. Avoid light exposure if the scaffold is UV-sensitive (unknown here).
• Sterility: For cell work, pass through a 0.22 µm PTFE/PES filter if compatible.

Aliquoting and stability (general):

• Prepare single-use aliquots to minimize freeze–thaw. Store aliquots tightly sealed under inert headspace if possible. Track preparation and expiry dates.
• Inspect solutions for discoloration or precipitation prior to use; discard if degradation is suspected.

Compliance: For research use only. Follow institutional SOPs and consult the SDS for detailed handling and stability information.

Structure & Identity

• Product name: AC-262536 (research-use small molecule)
• CAS: 870888-46-3 (Product Data)
• PubChem CID: 44512434 (Product Data)
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• InChIKey: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.

Structural features (general context):

• AC-262536 is widely cited in the literature as a nonsteroidal small molecule studied in androgen receptor research; however, item-specific structural descriptors are not provided here. Please consult the CoA/SDS for definitive identifiers.
• 2D structure description (general): Literature sources depict nonsteroidal AR-directed chemotypes as compact, heteroatom-containing scaffolds with multiple aromatic/aryl-ether or amide-like features; the exact ring systems, substituents, and stereochemistry for this item are not specified in the Product Data.

Identity confirmation (general guidance):

• For rigorous confirmation, users typically rely on LC–MS (exact mass), 1H/13C NMR, and HPLC purity profiles. Match against the supplier’s CoA for this specific lot.

Synthetic Utility

This item is offered as a defined bioactive small molecule rather than a synthetic building block. The Product Data do not specify functional handles or protecting groups amenable to downstream transformations.

• Not typically employed as a reagent, catalyst, or monomer in organic synthesis.
• If probe development or linker installation is desired, consult detailed structural data (CoA/SDS or primary literature) to identify positions that tolerate modification without abrogating activity. Absent item-specific structure here, no recommendations can be made.

General considerations (for chemists adapting bioactives):

• Late-stage functionalization strategies (C–H activation, photoredox, Minisci-type) may be explored where the scaffold permits; ensure you have unambiguous structural information before attempting.
• Maintain an analytical baseline (1H/13C NMR, HRMS, HPLC purity) before and after any derivatization to confirm identity and purity.
• If isotopically labeled analogs are needed for ADME research, select sites that minimize kinetic isotope effects while offering robust synthetic access.

Target Specificity

Not specified for this item; refer to CoA/Spec Sheet.

Note: Target, binding affinity, selectivity profiles, species reactivity, or pathway annotations are not provided in the Product Data for SKU A655766. Users should consult primary literature and perform in-house validation (e.g., reporter assays, binding assays) appropriate to their experimental system.

Frequently Asked Questions

How should this product be stored?

Store at ?80 °C. Ultra-low temperature storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 870888-46-3, the molecular formula is C18H18N2O, and the molecular weight is 278.35 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.