Modifying enzymes

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  1. 5' DNA/RNA Adenylase
    Liquid Animal Free ? Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material. Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Bioactive ? Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure. DNase, RNase free ? Free of DNase and RNase activity to keep DNA and RNA intact. Use in nucleic-acid extraction, PCR, and storage where degradation is a risk. ActiBioPure™ ? ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required. EnzymoPure™ ? EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance. Sterile ? Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization. 75 U/µL
    Out of Stock Item #: D743509
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    Species
    Adenosylase gene of thermophilic bacteria
    Accession #
    8UCH_A,A0A075LQ94
    Expression system
    E. coli
    Bioactivity
    One unit is the amount of enzyme required to catalyze the transfer of 1pmol of AMP to pDNA substrate in a total reaction volume of 20μl in 15 minutes at 65℃.
    Synonyms
    Single-Strand Nucleic Acid 5' Adenylase
  2. AlkB (RNA/DNA demethylase, Nuclease-free)
    Liquid Animal Free ? Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material. Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Bioactive ? Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure. ActiBioPure™ ? ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required. RNase free ? RNase-free — verified without RNase activity to protect fragile RNA. Use in RNA extraction, RT-PCR, and any RNA-sensitive workflow. EnzymoPure™ ? EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance. Sterile ? Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization. 20 U/µL
    In Stock Item #: A743741
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    Species
    Escherichia coli (strain K12)
    Accession #
    P05050.1
    Expression system
    E. coli
    Bioactivity
    One unit (U) is the amount of AlkB that removes 90-100% of the m6A modifications in total RNA of HEK 293T cells in 20 ul of reaction mixture at 37℃ after 100minutes of incubation. Under the regular conditions, the enzymatic activity of 1 μg of AlkB is approximately 1 unit.
    Synonyms
    alkB | aidD | ECK2204
  3. Antarctic Phosphatase (Thermosensitive)
    Liquid Animal Free ? Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material. Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Bioactive ? Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure. ActiBioPure™ ? ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required. EnzymoPure™ ? EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance. Sterile ? Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization. ≥90%(SDS-PAGE) 5.0 U/µl
    Out of Stock Item #: A743821
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    Species
    Antarctic bacterium TAB5
    Accession #
    2W5W_B,Q9KWY4
    Expression system
    E. coli
    Bioactivity
    1 unit refers to the 5 'concave end produced by restriction endonuclease digestion at 37 ℃ for 30 minutes. The enzyme amount required for dephosphorylation of µ g pUC19 vector DNA. The dephosphorylation standard refers to the self linking reaction Can inhibit>95% of DNA re circularization and detect it by transforming E. coli cells.
    Synonyms
    Thermosensitive phosphatase
  4. E.coli DNA Polymerase I
    EnzymoPure™ ? EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance. free of other DNA exonucleases or endonucleases, free of RNase.
    Out of Stock Item #: E745501
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  5. Klenow Fragment, Exo-
    Liquid Animal Free ? Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material. Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Bioactive ? Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure. ActiBioPure™ ? ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required. EnzymoPure™ ? EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance. Sterile ? Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization. 5 U/μL
    Out of Stock Item #: K747451
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    Species
    E. coli
    Accession #
    2KFZ_A,P00582
    Expression system
    E. coli
    Bioactivity
    One unit is the amount of enzyme required to catalyze the incorporation of 10nmol of deoxyribonucleotides (dNTPs) into a polynucleotide within 30 minutes at 37℃.
    Synonyms
    DNA polymerase I Fragment Mutant
  6. Lambda Exonuclease
    Liquid Animal Free ? Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material. Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Bioactive ? Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure. ActiBioPure™ ? ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required. RNase free ? RNase-free — verified without RNase activity to protect fragile RNA. Use in RNA extraction, RT-PCR, and any RNA-sensitive workflow. EnzymoPure™ ? EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance. Sterile ? Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization. ≥95%(SDS-PAGE) 5.0 U/μL
    Out of Stock Item #: L747582
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    Technical Identifiers
    Synonyms
    λ Exonuclease | exo | lambdap24
  7. SP6 RNA Polymerase
    Liquid Animal Free ? Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material. Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Bioactive ? Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure. ActiBioPure™ ? ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required. RNase free ? RNase-free — verified without RNase activity to protect fragile RNA. Use in RNA extraction, RT-PCR, and any RNA-sensitive workflow. EnzymoPure™ ? EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance. Sterile ? Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization. 20 U/µL
    Out of Stock Item #: S750679
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    Technical Identifiers
    Species
    Enterobacteria phage SP6
    Accession #
    Q7Y5N4
    Expression system
    E. coli
    Bioactivity
    One unit of enzyme incorporates 1nmol of AMP into polynucleotide in 1 hour at 37°C under the following activity assay conditions.
  8. T7 Endonuclease I
    Liquid Animal Free ? Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material. Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Bioactive ? Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure. ActiBioPure™ ? ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required. RNase free ? RNase-free — verified without RNase activity to protect fragile RNA. Use in RNA extraction, RT-PCR, and any RNA-sensitive workflow. EnzymoPure™ ? EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance. Sterile ? Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization. 10 U/µL
    Out of Stock Item #: T750878
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    Technical Identifiers
    Species
    Escherichia phage T7
    Accession #
    P00641.1
    Expression system
    E. coli
    Bioactivity
    One unit is defined as the amount of enzyme required to convert > 90% of 1μg of supercoiled cruciform pUC(AT) to > 90% linear form in a total reaction volume of 50 μl in 1 hour at 37℃.
    Synonyms
    T7 Endo I | T7EI | T7p19
  9. Terminal Deoxynucleotidyl Transferase (TdT)
    Liquid Bioactive ? Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure. DNase, RNase free ? Free of DNase and RNase activity to keep DNA and RNA intact. Use in nucleic-acid extraction, PCR, and storage where degradation is a risk. ActiBioPure™ ? ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required. EnzymoPure™ ? EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance. Sterile ? Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization. 5.0 U/μL
    Out of Stock Item #: T751007
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    Technical Identifiers
    Species
    Bovine
    Accession #
    P06526
    Expression system
    E. coli
    Bioactivity
    One Unit is the amount of enzyme required to catalyze the incorporation of 1 nmol of dNTP to the 3'-OH termini of a polynucleotide within 60 minutes at 37℃.
    Synonyms
    TdT | DNTT | ‌ DNA nucleotidylexotransferase‌‌ | Terminal addition enzyme‌‌ | Terminal deoxynucleotidyltransferase‌‌ ...
  10. Terminal Deoxynucleotidyl Transferase (TdT)
    CAS: 9027-67-2 EC Number: 232-819-6
    Liquid Bioactive ? Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure. DNase, RNase free ? Free of DNase and RNase activity to keep DNA and RNA intact. Use in nucleic-acid extraction, PCR, and storage where degradation is a risk. ActiBioPure™ ? ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required. EnzymoPure™ ? EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance. Sterile ? Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization. 20 U/μL
    Out of Stock Item #: T751008
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    Pricing & Pack Sizes
    Technical Identifiers
    Species
    Bovine
    Accession #
    P06526
    Expression system
    E. coli
    Bioactivity
    One unit is the amount of enzyme required to catalyze the incorporation of 1 nmol of dNTP to the 3'-OH termini of a polynucleotide within 60 minutes at 37℃.
    Synonyms
    TdT | DNTT | ‌ DNA nucleotidylexotransferase‌‌ | Terminal addition enzyme‌‌ | Terminal deoxynucleotidyltransferase‌‌ ...
  11. Thermostable RNase H
    Liquid Suitable for molecular biology ? Molecular-biology grade — free of nucleases and contaminants that degrade DNA/RNA. Use in cloning, PCR, and nucleic-acid work needing clean reagents. BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility. DNase, RNase free ? Free of DNase and RNase activity to keep DNA and RNA intact. Use in nucleic-acid extraction, PCR, and storage where degradation is a risk. EnzymoPure™ ? EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance. Sterile ? Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization. for DNA and RNA applications ? For nucleic-acid (DNA & RNA) applications — nuclease-controlled across both. Use in workflows handling DNA and RNA together where degradation is a risk. ≥95% 5 U/μl
    Out of Stock Item #: T751065
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    Technical Identifiers
    Synonyms
    Thermostable RNase H | RNase H
  12. Recombinant Cas9 Nuclease (SpCas9)
    CAS: 1425049-49-5
    Liquid Animal Free ? Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material. Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Recombinant ? Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin. RNase free ? RNase-free — verified without RNase activity to protect fragile RNA. Use in RNA extraction, RT-PCR, and any RNA-sensitive workflow. EnzymoPure™ ? EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance. Sterile ? Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization. 1μM (158ng/μL )
    Out of Stock Item #: C744422
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    Technical Identifiers
    Species
    Streptococcus pyogenes serotype M1
    Accession #
    Q99ZW2.1
    Expression system
    E. coli
    Synonyms
    CRISPR-associated endonuclease Cas9 | Csn1
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R&D Use Only Products supplied for research and development use

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