ANT3310 sodium - ≥99% , CAS No.2410688-61-6

CAS: 2410688-61-6 Cat. No.: A649786 Formula: C₆H₈FN₂NaO₅S Molecular Weight: 262.19

GRADE & PURITY ≥99%

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

ANT3310 sodium is a broad-spectrum covalent Serine β-Lactamase inhibitor, with IC 50 values ranging from 1 nM to 175 nM (a panel of Serine β-Lactamase). ANT3310 sodium potentiates activity of β-lactam antibiotics against Carbapenem-Resistant Enterobacterales (CRE) and Acinetobacter baumannii (CRAB). ANT3310 sodium can be used in the research of bacterial infection.

In Vitro

ANT3310 sodium (Compound 21, 0.006 to 3 000 nM, 10 min) inhibits a series of Serine β-Lactamase (AmpC, CTX-M-15, TEM-1, OXA-48, OXA-23, and KPC-2), with IC <>50 values ranging from 1 nM to 175 nM. ANT3310 sodium shows a low in vitro cytotoxicity (IC <>50 : > 100 μM) in HepG2 cell, cardiotoxicity (inhibition of the hERG potassium ion channel), and genotoxicity (Ames test). MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

ANT3310 sodium (intravenous injection, 25-100 mg/kg, at 1, 3, 5, and 7 h postinfection) reduces bacterial burdens in murine thigh infection model . ANT3310 sodium (intravenous injection, 1 mg/kg, Male Swiss albino mice) shows a T 1/2 value of 0.64 h, AUC value of 412 ng•h/mL, and Cl value of 40 mL/min/kg (pharmacokinetic assay) . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Murine thigh infection model Dosage: 25, 50, and 100 mg/kg Administration: Intravenous injection, at 1, 3, 5, and 7 h postinfection Result: Reduced bacterial burdens (colony forming units, CFU) in a dose-dependent manner to levels below that of the initial starting inoculum at the highest dose, when treated with the combination of MEM.

Form:Solid

Specifications

Specifications & Purity

≥99%

Biochemical and Physiological Mechanisms

ANT3310 sodium is a broad-spectrum covalent Serine β-Lactamase inhibitor, with IC 50 values ranging from 1 nM to 175 nM (a panel of Serine β-Lactamase). ANT3310 sodium potentiates activity of β-lactam antibiotics against Carbapenem-Resistant Enterobactera

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Action Type

INHIBITOR

Purity

≥99%

Names and Identifiers

Molecular Weight

262.19

Chemical and Physical Properties

• Solubility: DMSO : 250 mg/mL (953.51 mM; Need ultrasonic)

Application Protocols

No tested applications or validated protocols are provided in the Product Data for ANT3310 sodium. The following general practices apply to small-molecule library compounds (not item-specific):

• Stock preparation: Weigh accurately under dry conditions. Dissolve in anhydrous DMSO to a convenient concentration (e.g., 10–50 mM). Record salt form and exact mass basis.
• Storage aliquots: Dispense into single-use aliquots (amber vials or plates) to avoid repeated freeze–thaw. Headspace minimization and inert gas overlay (e.g., nitrogen) can improve stability.
• Working solutions: Dilute into assay buffer or media with thorough mixing to prevent precipitation. Maintain final DMSO content within assay tolerance.
• Quality checks: Run LC-MS/HPLC on fresh and aged stocks to confirm stability. Check pH/osmolarity of final media if dosing volumes are high.
• Documentation: Track batch/lot number, preparation date, and storage conditions for data integrity.

For any application requiring validated conditions (e.g., enzymatic IC50 determination), develop and qualify protocols internally with proper controls. This product is supplied for research use only.

Biological Roles

Item-specific biological function, targets, or pathway roles are not specified for ANT3310 sodium. No claims of biological activity are made in this catalog entry. For research use only.

General notes for small-molecule library members (not item-specific):

• Library compounds are commonly evaluated as chemical probes in cellular or cell-free systems to interrogate pathways or phenotypes. Their roles, if any, are determined empirically during screening.
• If the compound is an ionic sodium salt, its behavior in biological buffers may differ from that of the neutral acid due to charge state and transport properties (general chemistry). Effects such as altered membrane permeability or protein binding are structure-dependent.
• When testing in biological systems, confirm the absence of assay-interfering behavior (e.g., aggregation, redox cycling, metal chelation, detergent-like effects) using orthogonal readouts and counterscreens.
• Ensure compatibility with assay media (pH, osmolarity, ionic strength) and verify that the sodium counterion does not confound control conditions—typically negligible at low micromolar dosing but should be tracked in sensitive systems.

Researchers should consult the CoA/Spec Sheet and conduct pilot studies to define any specific biological role or mechanism for this item.

Buffer Applications

This product is a discrete small molecule and is not a classical buffer component. Specific buffer system usage and pKa values are not specified for this item.

General guidance (not item-specific):

• If preparing aqueous working solutions for assays, dissolve first in DMSO or a compatible co-solvent, then dilute into the desired buffer (e.g., PBS, HEPES, Tris) while monitoring for precipitation.
• Maintain buffer pH where the compound is known to be stable (unknown here). Avoid strong acid/base adjustments unless stability has been confirmed.
• Filter sterilize (0.22 µm) only if the compound is stable to brief filtration and adsorption losses are acceptable; pre-rinse filters with solvent to reduce binding.

For actual buffer formulation data (pKa, buffering ranges), consult the specific compound’s structural information when available; not specified for this item.

Green Alternatives

This listing is a discrete small-molecule entity rather than a process solvent or bulk reagent. As such, “green alternative” selection centers on the workup and analytical context rather than replacement of the compound itself.

• Solvent choices: When making stocks or running analytics, prefer water or biodegradable/alcoholic solvents (e.g., ethanol) where compatible with solubility and stability (general guidance). Reduce reliance on high-toxicity solvents (e.g., DMF, dichloromethane) when practical.
• Minimize waste: Use micro-scale solubility screens, prepare only the volume needed, and consolidate analytical runs.
• Energy efficiency: Product data specify storage at -20°C. Limit door-open time, use aliquots to avoid repeated freeze–thaw cycles, and ship consolidated orders to reduce cold-chain footprint.
• Safer analytics: Favor UPLC over HPLC (reduced solvent consumption) and consider SFC where compatible with ionizable analytes (method development required; general note).

Comparison (general; not item-specific)

• DMSO vs DMF: DMSO is typically less hazardous and broadly effective for stock solutions; choose DMSO when feasible.
• Ethanol/water vs ACN/MeOH: Where solubility permits, EtOH/H2O can reduce toxicity; however, ACN/MeOH often provide superior chromatographic performance. Balance EHS with data quality needs.

Given the unknown structure, any greener substitution must be validated experimentally for solubility, stability, and assay performance.

Pharmaceutical Uses

No pharmacopeial grade or excipient role is specified for ANT3310 sodium. This product is supplied strictly for research use only and is not intended for human or veterinary use, diagnostic procedures, or as an active pharmaceutical ingredient (API).

General formulation context for small molecules (not item-specific):

• In preclinical research, sodium salt forms may be explored to improve aqueous solubility and handling; however, any formulation development must be supported by full structural and stability data for the specific compound.
• If used in dosage-form feasibility studies (laboratory only), typical vehicles include aqueous buffers, cosolvent systems (e.g., DMSO/PEG400/saline), or cyclodextrin complexes. Such uses require independent verification of safety and suitability and are outside the scope of this catalog entry.

No claims are made regarding compendial compliance (USP/EP/JP), residual solvent classes, elemental impurities, or endotoxin/bioburden for this item. Refer to the CoA/Spec Sheet for any available quality attributes.

Physical Properties

Item-specific specifications

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Formula: Not specified for this item; refer to CoA/Spec Sheet.
• Melting Point (MP): Not specified for this item; refer to CoA/Spec Sheet.
• Boiling Point (BP): Not specified for this item; refer to CoA/Spec Sheet.
• Density: Not specified for this item; refer to CoA/Spec Sheet.
• Refractive Index: Not applicable/unknown; refer to CoA/Spec Sheet.
• pKa/logP: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Not specified for this item; refer to CoA/Spec Sheet.

Literature/general guidance (not item-specific)

• Sodium salts of organic molecules are often more polar and can display enhanced aqueous solubility relative to their neutral acid forms (literature, general trend). However, solubility is highly structure-dependent and can range from sparingly soluble to freely soluble.
• If the counterion is sodium and the conjugate base is a carboxylate or sulfonate, the solid is commonly crystalline and thermally stable under ambient dry conditions (general chemistry). Hygroscopicity varies widely.
• For unknown small molecules intended for screening libraries, a common practice is to evaluate solubility in DMSO (anhydrous) first, then assess water/PBS compatibility by stepwise dilution (general practice), monitoring for precipitation.

Always verify item-specific physical properties using the product CoA/Spec Sheet when available; do not substitute general trends for specifications.

Quality & Grades

Item-specific grade/purity

• Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.

How to interpret grades (general guidance)

• Analytical grades (e.g., AR, ACS) indicate compliance with defined impurity limits suitable for quantitative analysis.
• Screening-library or research grades prioritize identity and purity sufficient for bioassays and discovery chemistry; low non-volatile residue and accurate salt/stoichiometry are typical expectations.
• For chromatography-sensitive applications (e.g., LC-MS bioassays), low background and defined counterion content are important; a certificate with HPLC purity and NMR identity is often requested.

Recommended quality controls (practical tips)

• Request and review the CoA for batch-specific purity (HPLC/UPLC), identity (1H NMR, MS), and water/volatile content (e.g., KF), when available. For this catalog entry, any unspecified parameter should be considered “Not specified for this item; refer to CoA/Spec Sheet.”
• If dose–response data quality is critical, consider in-house verification: LC-MS for identity and purity, qNMR for assay, and IC for sodium quantitation where relevant (general practice).

Note: Stabilizers, residual solvents, metal limits, and UV cutoffs are not specified for this item; consult the CoA/SDS for details if applicable.

Reaction & Applications

Manufacturer Applications

• Not specified for this item in the catalog entry beyond inclusion in a small-molecule/compound library. Use and expand on this as appropriate for discovery workflows.

Discovery and screening use (general, not item-specific)

• Compounds in screening libraries are typically employed in biochemical, cell-based, or biophysical assays to explore structure–activity relationships (SAR) and identify tool compounds. As composition is unspecified here, treat ANT3310 sodium as a generic library member for method planning.

Practical workflow tips

• Prepare mass-verified stock solutions (e.g., 10 mM in DMSO) with accurate weighing or qNMR assay; document salt form and any hydrate assumptions.
• Use serial dilution in compatible assay media; maintain final DMSO ≤0.1–1% v/v where assay sensitivity requires (general best practice).
• Confirm chemical stability across assay time via LC-MS/HPLC (look for degradation, salt exchange, or aggregation-induced artifacts).
• Implement controls for interference (fluorescence quenching, redox cycling, aggregation); use detergent-containing buffers or orthogonal assays when necessary.

Synthetic/reagent use

• Without a defined structure or functional group profile, ANT3310 sodium is not recommended as a general synthetic reagent. If synthetic transformation is intended, first obtain the full structural and stability data from the CoA/Spec Sheet and pilot conditions on milligram scale.

Reaction Conditions

Item-specific reaction conditions, catalysts, or yields are not specified for ANT3310 sodium. No particular reactivity profile is claimed in this listing.

General laboratory handling for small-molecule salts (not item-specific):

• If solution-phase manipulations are required (e.g., salt exchange, counterion swap), consider mild conditions in polar solvents such as water, methanol, ethanol, acetonitrile, or DMSO, followed by appropriate workup (lyophilization or precipitation) depending on solubility.
• Monitor integrity by HPLC/UPLC and MS. Where tautomerism or acid/base equilibria are possible, control pH and ionic strength to minimize decomposition.
• Avoid excessive heating or extended exposure to moisture unless thermal/hydrolytic stability is confirmed for the specific batch (unknown here). Store at -20°C per Product Data when not in use.

Any concrete reaction planning should be deferred until structural and stability details are available from the CoA/Spec Sheet.

Safety & Handling

GHS and hazard information (item-specific)

• Signal Word: Not specified for this item; refer to SDS.
• H-Statements: Not specified for this item; refer to SDS.
• GHS Classification: Not specified for this item; refer to SDS.
• Pictograms: Not specified for this item; refer to SDS.

Always consult the latest SDS for authoritative safety information before use.

General laboratory precautions (not item-specific)

• Handle in a fume hood or well-ventilated area. Avoid dust generation and inhalation.
• Wear appropriate PPE: lab coat, safety glasses, and compatible chemical-resistant gloves.
• Avoid contact with skin and eyes. Wash hands thoroughly after handling.
• Prevent contamination of reagents and avoid ingestion. For research use only.

Storage and incompatibilities

• Product Data indicates: Store at -20°C. Maintain in tightly sealed original packaging, protected from moisture and light.
• Potential incompatibilities depend on structure; as a general precaution, keep away from strong oxidizers and strong acids until specific compatibility is known.

First-aid overview (general)

• Inhalation: Move to fresh air; seek medical attention if symptoms occur.
• Skin/eye contact: Rinse with water for at least 15 minutes; remove contaminated clothing; seek medical attention if irritation persists.
• Ingestion: Rinse mouth; do not induce vomiting; seek medical attention.

Spill response: Avoid raising dust; collect with non-sparking tools into compatible container for disposal per local regulations.

Solvent Selection

Item-specific solubility

• Not specified for this item; refer to CoA/Spec Sheet.

General selection strategy for small-molecule sodium salts (literature/practice, not item-specific)

• Start with DMSO (anhydrous) to prepare concentrated stocks (e.g., 10–50 mM), as it solubilizes a broad range of polar/ionic organics.
• For aqueous applications, dilute DMSO stocks into water, PBS, or assay buffers while mixing to minimize precipitation. Filter through 0.22 µm if compatible.
• If water solubility is poor, evaluate mixed solvents: DMSO/H2O, DMF/H2O, or ethanol/H2O, adjusting ionic strength and pH only if compatible with the molecule’s stability (unknown here).
• For strictly non-aqueous operations (e.g., analytical prep), test MeOH, ACN, or acetone. Avoid protic acids/bases unless compatible with the compound’s integrity.

Polarity and miscibility (general notes)

• Sodium salts are typically more hydrophilic than their neutral analogs; however, bulky hydrophobes or multiple aromatic rings can still limit water solubility.
• Assess hygroscopicity by mass change on exposure to air; store promptly after opening if hygroscopic.

Because the exact structure is not provided, finalize solvent choice empirically using a small-scale solubility screen and stability checks (LC-MS, HPLC peak shape) before committing to large-scale use.

Storage & Reconstitution

Storage (item-specific)

• Product Data: Store at -20°C.
• Shipped in: Ice chest + ice pads (cold-chain). Upon receipt, place immediately in a -20°C freezer. Keep container tightly closed, protected from moisture and light.

Reconstitution (general guidance; not item-specific)

• Allow vial to equilibrate to room temperature in a desiccator before opening to minimize condensation.
• For initial dissolution, use anhydrous DMSO or another suitable solvent based on solubility screening; actual solvent recommendations are not specified for this item.
• Prepare concentrated stock solutions (e.g., 10–50 mM) when feasible. Vortex and, if necessary, brief sonication to aid dissolution. Avoid heating unless stability is confirmed.
• If aqueous solutions are required, dilute organic stocks slowly into water or buffer with mixing. Inspect for precipitation; filter (0.22 µm) only if compatible.

Aliquoting and stability (general)

• Dispense into single-use aliquots to avoid freeze–thaw cycles. Store aliquots at -20°C (or per any future item-specific guidance on the CoA/Spec Sheet).
• Track preparation date and solvent. Periodically verify integrity by HPLC/LC-MS.

Item-specific details such as hydrate state, water content, and exact solubility are not specified for this item; refer to CoA/Spec Sheet.

Structure & Identity

Overview

• SKU: A649786
• Product Name: ANT3310 sodium
• CAS: 2410688-61-6
• Category: Small molecules and compound libraries (研究/筛选用途)
• Exact chemical identity: Not specified for this item; refer to CoA/Spec Sheet.

Identifiers

• Molecular Formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• InChIKey: Not specified for this item; refer to CoA/Spec Sheet.
• CID: Not specified for this item; refer to CoA/Spec Sheet.

Structural Features (general chemistry notes)

• The designation “sodium” typically denotes a sodium salt of an anionic small molecule (general chemistry). Such salts often improve crystallinity and water compatibility; however, the presence, position, and identity of the anionic functionality (e.g., carboxylate, sulfonate, phenolate) are not specified for this item.
• Without a provided structure, specific functional groups, ring systems, stereochemistry, and 2D atom connectivity cannot be described for this product. Please consult the product’s CoA/Spec Sheet when available for definitive structural information.

2D Structure (descriptive)

• Not specified for this item; refer to CoA/Spec Sheet.

Synthetic Utility

Specific synthetic reactivity and functional group profile for ANT3310 sodium are not specified. Without a defined structure, this product should not be assumed to function as a general reagent, catalyst, or building block.

General considerations (not item-specific):

• Sodium salts of organic molecules may represent the conjugate bases of acids (e.g., carboxylates, phenolates, sulfonates). Their nucleophilicity/basicity and synthetic value vary widely with structure.
• If a transformation is contemplated (e.g., salt metathesis, ion exchange, phase-transfer operations), first obtain definitive structural data and assess compatibility with proposed conditions on a small scale.
• For purification, sodium salts often display increased polarity, enabling separation by ion-exchange or reverse-phase chromatography; yet volatility is typically low, precluding distillation.

In the absence of structural information, ANT3310 sodium should be treated primarily as a library compound for screening rather than a synthetic reagent.

Target Specificity

Target, pathway, or enzyme specificity for ANT3310 sodium is not specified in the Product Data. No claims are made regarding biochemical targets, binding constants, or selectivity profiles.

Guidance for researchers (general, not item-specific):

• Establish specificity experimentally using orthogonal assays (biochemical enzymology, biophysics, cell-based phenotypes) and include counter-screens against common off-targets relevant to your modality.
• Validate with dose–response curves, confirm identity and purity of the working stock, and assess potential assay interference mechanisms (fluorescence, redox, aggregation).

Consult the CoA/Spec Sheet and your internal screening data to define any target-related attributes for this compound.