APN-Azide - ≥95% , CAS No.1643841-88-6

CAS: 1643841-88-6 Cat. No.: A467144 Formula: C₉H₄N₄ Molecular Weight: 168.15 EC Number: 110-155-7

GRADE & PURITY ≥95%

Synonyms

APN-N₃, 3-(4-Azidophenyl)propiolonitrile

Storage

Store at 2-8°C,Protected from light

Shipped In

Wet ice

Overview

Description

APN-Azide is a bifunctional crosslinker for thiol-to-alkyne coupling. This coupling can be performed with high selectivity in biological medium using mild reaction conditions.Standard protein labeling procedure (cysteine labeling)1. Dissolve the protein in the appropriate buffer* with pH 6.5-9.0 (e.g. PBS) at 1-10 mg/mL concentration.2. Apply the appropriate amount of the stock solution of the reagent (1-5 molar eq. per free cysteine residue).3. Incubate at room temperature for 2 hours.4. If necessary, purify the protein-azide conjugate using size exclusion chromatography or ultrafiltration.5. The conjugate can be readily coupled with alkyne-containing using standard click-chemistry protocols.*Note: avoid thiol-containing buffers.

Specifications

Synonyms

APN-N₃, 3-(4-Azidophenyl)propiolonitrile

Specifications & Purity

≥95%

Storage

Store at 2-8°C,Protected from light

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Purity

≥95%

Names and Identifiers

• Canonical Smiles: N#CC#CC1=CC=C(N=[N+]=[N-])C=C1
• InChIKey: BDLXITYEYYHQNK-UHFFFAOYSA-N
• INCHI: 1S/C9H4N4/c10-7-1-2-8-3-5-9(6-4-8)12-13-11/h3-6H
• UN Number: 1325
• Molecular Weight: 168.15
• Reaxy-Rn: 27679920
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=27679920&ln=

Chemical and Physical Properties

• Sensitivity: light sensitive
• Flash Point(°F): Not applicable
• Flash Point(°C): Not applicable
• Melt Point(°C): 100℃