Avidin from egg white, CAS No.1405-69-2

CAS: 1405-69-2 Cat. No.: A103033 EC Number: 215-783-6

GRADE & PURITY Bioactive, Native, ≥98%(SDS-PAGE), ≥10 U/mg solid; >10 U/mg protein; ≥0.9 mg protein/mg solid (see COA for assay result)

Accession #

P02701

Bioactivity

≥10 U/mg solid; >10 U/mg protein

Overview

Avidin is a tetrameric protein. In chicken egg white, avidin makes up approximately 0.05% of total protein (approximately 1.8 mg per egg). The tetrameric protein contains four identical subunits (homotetramer) each of which can bind to biotin (Vitamin B7, vitamin H) with a high degree of affinity and specificity.

Avidin's affinity for biotin is exploited in wide ranging biochemical assays, including Western Blot, ELISA, ELISPOT and pull-down assays. Avidin immobilized onto solid supports is also used as purification media to capture biotin-labelled protein or nucleic acid molecules. For example, cell surface proteins can be specifically labelled with membrane impermeable biotin reagent, then specifically captured using an avidin-based support.

Avidin and biotin are routinely conjugated to indicator enzymes, such as alkaline phosphatase and horseradish peroxidase for use in diagnostic kit development and manufacturing.

Application：

Avidin from egg white can be used in assays for functionalized xenon as a biosensor to detect biotin-avidin binding. Egg white was used at 80 nmol.

Specifications

Product Name

Avidin from egg white, CAS No.1405-69-2

Synonyms

Avidin from egg white | Avidin, chiken egg white | Avidin, Egg White | Anti-biotin protein | Immunopure Avidin | AVD

Grade

Bioactive, Native

Specifications & Purity

Bioactive, Native, ≥98%(SDS-PAGE), ≥10 U/mg solid; >10 U/mg protein; ≥0.9 mg protein/mg solid (see COA for assay result)

Biochemical and Physiological Mechanisms

The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin). Post-translational: N-linked glycan at Asn-41 consists of GlcNAc(beta1-2)Man(alpha1-3)[GlcNAc(beta1-4)

Bioactivity

≥10 U/mg solid; >10 U/mg protein

Accession #

P02701

Predicted molecular weight

64,000 (tetramer of 16,000 MW subunits)

CAS

1405-69-2

Molecule Type

Protein

Product Line

Biological Products

Storage and Shipping

Concentration

≥10 U/mg solid; >10 U/mg protein; ≥0.9 mg protein/mg solid (see COA for assay result)

Reconstitution

Reconstitute in 0.1 M phosphate buffer, pH 6.8.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (36 months). Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.

Unit definition

One unit will bind one microgram of d-biotin.