Bacteriophage SM Buffer - BioReagent, Sterile, Suitable for molecular biology

Cat. No.: B1533124

GRADE & PURITY BioReagent, Suitable for molecular biology, Sterile

Synonyms

SM buffer | SM Buffer for bacteriophage | Phage SM Buffer

Storage

Store at 2-8°C

Shipped In

Wet ice

Application

1106

Overview

Bacteriophage λ is one of the earliest cloning vectors. Its genome is a double-stranded DNA of approximately 50 kb. Bacteriophage λ exhibits two life cycles within host cells: ① Lytic growth: the circular phage DNA replicates extensively in the host cell, synthesizes phage gene products and assembles into mature phage particles. Eventually, the host cell is lysed to release progeny phages for subsequent infection. ② Lysogenic growth: λ phage DNA integrates into the host chromosomal DNA and replicates together with the host genome, passing on to daughter cells without lysis of the host. In research, the lytic cycle of bacteriophage λ is commonly utilized to propagate high-titer phage particles for λ phage DNA extraction.

Bacteriophage SM Buffer consists mainly of magnesium sulfate, sodium chloride and gelatin. It is autoclaved to obtain a sterile solution, and is used for dilution and storage of bacteriophage λ stocks. For research use only. Not for clinical diagnosis or other applications.

Procedure (For reference only):

1. Operate according to specific experimental requirements.

2. Since this reagent contains gelatin, it may form a gel-like substance at low temperatures. Incubate at 25–37 °C until completely dissolved, mix thoroughly before use.

Precautions:

1. Maintain aseptic technique to avoid contamination.

2. Use the reagent promptly after opening to prevent compromised experimental performance.

3. For your safety and health, wear a lab coat and disposable gloves during operation.

Specifications

Synonyms

SM buffer | SM Buffer for bacteriophage | Phage SM Buffer

Specifications & Purity

BioReagent, Sterile, Suitable for molecular biology

Stability And Storage

Store at 2-8℃ long term (12 months).

Storage

Store at 2-8°C

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, Sterile, Suitable for molecular biology

Product Line

Biological Products