BI8622 - 10mM in DMSO , CAS No.1875036-74-0

CAS: 1875036-74-0 Cat. No.: B655041 Formula: C₂₅H₂₆N₆O Molecular Weight: 426.51 PubChem CID: 138377590

GRADE & PURITY 10mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Overview

BI8622 is a specific inhibitor of the ubiquitin ligase HUWE1 with an IC 50 of 3.1 μM

In Vitro

BI8622 induces HUWE1 ectopically expresses to abolish ubiquitination of MCL1 with an IC 50 value of 6.8 μM in HeLa cells. BI8622 suppresses colony formation of Ls174T cells with estimated IC 50 value of 8.4 μM. BI8622 (10 μM; 1-4 days) treatment retards passage of Ls174T cells through all phases of the cell cycle, with the effect being strongest for G1. BI8622 (0-50 μM; 16 hours) retards the degradation of MCL1 in response to UV irradiation by inhibiting HUWE1 in HeLa cells. BI8622 inhibits MYC-dependent transactivation in colorectal cancer cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Cycle AnalysisCell Line: Ls174T cells Concentration: 0 μM, 5 μM,10 μM, 15 μM, 20 μM Incubation Time: 0-4 days Result: Retarded passage of Ls174T cells through all phases of the cell cycle, with the effect being strongest for G1. Western Blot AnalysisCell Line: HeLa cells Concentration: 0 μM, 10 μM, 20 μM Incubation Time: 16 hours Result: Retarded the degradation of MCL1 in response to UV irradiation by inhibiting HUWE1 in HeLa cells.

IC50& Target:IC50: 3.1 μM (HUWE1)

Specifications

Specifications & Purity

10mM in DMSO

Biochemical and Physiological Mechanisms

BI8622 is a specific inhibitor of the ubiquitin ligase HUWE1 with an IC 50 of 3.1 μM.

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

This product requires cold chain shipping. Ground and other economy services are not available.

Action Type

INHIBITOR

Names and Identifiers

PubChem CID

138377590

Molecular Weight

426.51