Brain Infusion Powder - BioReagent,Suitable for microbiology

Cat. No.: B1522335

GRADE & PURITY BioReagent, Suitable for microbiology

Synonyms

Bovine Brain Extract Powder

Storage

Room temperature

Shipped In

Normal

Application

Microbial culture

Overview

This product is a pale yellow to off-white powder made from fresh bovine brain through extraction, concentration and spray drying. It is hygroscopic and readily soluble in water. As a basic material for preparing various culture media, it provides growth factors required for bacterial growth.

Directions for Use:

Use as a raw material for culture media. Dissolve thoroughly and use after sterilization.

Conventional Physical and Chemical Indicators:

Organoleptic Indicators

Item	Requirements
Form	Powdered, no caking
Color	Light yellow or brown powder
Odor	Characteristic odor of the product, no other off-odors
Impurities	No visible foreign impurities under normal vision

Physical and Chemical Indicators

Item	Reagent Grade
Total Nitrogen (dry basis), %	≥8.0%
Amino Nitrogen (dry basis), %	≥2.0%
Ash (dry basis) %	≤13.0%
Moisture %	≤6.0%
Nitrate & Alkaline Autoclave Test	Clear
Transparency (2% aqueous solution)	Clear

Specifications

Synonyms

Bovine Brain Extract Powder

Specifications & Purity

BioReagent,Suitable for microbiology

Stability And Storage

Store at room temperature long term (36 months).

Storage

Room temperature

Shipped In

Normal

Grade

BioReagent, Suitable for microbiology

Product Line

Biological Products

Application Protocols

No item‑specific, tested application protocols are provided in the Product Data. For preparation and use, follow your laboratory’s validated SOPs or recognized compendial methods for formulating infusion‑type media. Consult the product’s CoA/Spec Sheet for any lot‑specific guidance.

Biological Roles

Brain Infusion Powder does not represent a single biomolecule with a specific cellular role; rather, it is a nutrient mixture that provides multiple classes of biological building blocks to cultured microorganisms.

• Functional contributions in culture (general/literature):

  • Nitrogen sources: Short peptides and free amino acids support protein synthesis and rapid growth.
  • Carbon/energy: Small organics (e.g., amino acids, peptides, residual carbohydrates) furnish carbon and energy; additional carbohydrates are often added in full media recipes to tune metabolism.
  • Growth factors: Vitamins, nucleotides, and trace elements present in tissue infusions can enhance viability of fastidious organisms.
  • Osmotic and buffering effects: In complete media, added salts and phosphates regulate osmolarity and pH; the infusion component itself contributes modest ionic content.

• Metabolic context (general):

  • Supports heterotrophic metabolism, enabling high biomass yields in batch culture and improved recovery from stressed states (e.g., post‑preservation or environmental exposure).

• Scope and limitations:

  • As an undefined mixture, precise concentrations of individual nutrients vary by lot within controlled ranges; organism responses may require empirical optimization (e.g., supplementing with hemin, NAD, or carbohydrates when indicated by literature/SOPs).

This product is intended for research use only and is not intended to diagnose, treat, cure, or prevent any disease.

Buffer Applications

Not typically used as a standalone buffering agent. Brain Infusion Powder is a nutrient base rather than a defined buffer system.

• Practical notes (general/literature):
  • When formulating media, buffering capacity is generally provided by added salts (e.g., phosphates) in the complete recipe; the infusion component contributes limited, variable buffering.
  • If pH control is critical (e.g., during fermentation or extended incubation), incorporate an appropriate buffer system per validated methods or use controlled CO2/HCO3− systems where indicated.

For recipes and pH targets, follow compendial or institutional SOPs for the specific medium type you are preparing. Do not treat this product as a calibrated buffer standard.

Green Alternatives

Sustainability considerations for Brain Infusion Powder focus on source materials and waste handling rather than solvent hazards.

• Potential greener choices (general/literature):

  • Plant‑based complex media: Soy/vegetable peptones and yeast extract can replace animal‑derived infusions in many applications, reducing reliance on animal tissues and easing certain regulatory constraints.
  • Chemically defined media: Eliminate animal components and improve batch reproducibility, though may require optimization for fastidious strains.

• Trade‑offs:

  • Performance: Animal‑derived infusions can support growth of especially demanding organisms; switching to plant/defined formulations may alter growth rates or expression profiles.
  • Traceability: Defined and plant‑based media offer clearer compositional control; undefined infusions may vary within controlled limits.
  • Waste minimization: Prepare only the volume required; autoclave and dispose of spent media responsibly to minimize biological and chemical waste.

• Comparison snapshot (general; not item‑specific):

  • Brain Infusion Powder: rich, undefined nutrient profile; strong growth promotion for fastidious organisms; animal‑derived sourcing typical.
  • Yeast/Soy‑based media: animal‑component‑free; improved sustainability; may need supplementation for certain organisms.
  • Defined media: maximal reproducibility and traceability; may provide narrower organism coverage without further optimization.

Choose based on organism needs, regulatory considerations, and institutional sustainability goals.

Pharmaceutical Uses

No therapeutic or clinical claims are made for this product. For research use only (per Product Data).

• Relevant roles in pharmaceutical/biotech laboratories (general):

  • Microbiological quality control workflows: preparation of enrichment media used in bioburden monitoring, environmental monitoring, and growth promotion testing of neutralizing/transport media (per internal SOPs/compendia).
  • Process development research: cultivation of non-pathogenic strains for method development, media comparison studies, or recovery studies.

• Compliance and sourcing:

  • If compendial conformity (e.g., USP/EP) or animal‑component‑free sourcing is required for your application, verify on the lot‑specific CoA/Spec Sheet. This listing does not assert compendial status.

• Formulation/excipient status:

  • Not used as an excipient in finished dosage forms. Its role is limited to upstream microbiology research and QC laboratory media preparation.

Physical Properties

• Item-specific properties (Product Data):

  • Appearance: Not specified for this item; refer to CoA/Spec Sheet.
  • Storage Conditions: Room temperature (per Product Data).
  • Shipped In: Normal (per Product Data).

• Typical physical characteristics (general/literature; not item‑specific specs):

  • Physical form: Dry, free‑flowing powder intended for rapid dissolution in purified water when preparing media.
  • Solubility: Freely soluble/dispersible in water; trace insoluble particulate may remain and is normally removed by clarification or is tolerated during autoclaving (literature). Insoluble in common organic solvents.
  • Hygroscopicity: Prone to moisture uptake; caking may occur upon exposure to humidity (general observation for complex protein hydrolysates/infusions).
  • Color/odor: Often light tan to beige with characteristic proteinaceous odor (literature). Do not treat as specification.

• Solution behavior (general/literature):

  • Aqueous solutions are typically near‑neutral when used within standard infusion or brain‑heart infusion (BHI) formulations, but final pH depends on complete recipe and water quality.
  • Heat sterilization (e.g., autoclaving) can induce Maillard browning; mild color change is common and not necessarily indicative of performance loss.

For exact numeric parameters (e.g., moisture content, ash, chloride), consult the item’s CoA/Spec Sheet; values are manufacturer- and lot‑specific for undefined mixtures.

Quality & Grades

• Item-specific grade (Product Data): BioReagent, Suitable for microbiology.

• What this generally implies (general explanation):

  • Formulated and controlled for use in microbiological applications (culture media, enrichment, recovery). Emphasis on performance attributes such as support of target organism growth, freedom from inhibitory contaminants at levels relevant to microbiology, and consistency across lots.
  • BioReagent grade indicates suitability for research use in biological systems; not intended for clinical diagnostics or therapeutic applications.

• Quality controls typically associated with this type of product (literature/general; not item‑specific):

  • Growth promotion testing (GPT) with representative fastidious and non‑fastidious organisms under defined conditions.
  • Physicochemical checks (e.g., moisture, ash, total nitrogen) and sensory profile (color/odor) within internal ranges.
  • Microbiological bioburden/endotoxin controls as appropriate for the intended research use (not necessarily compendial unless stated on CoA).

• Documentation and traceability:

  • For definitive specifications, acceptance criteria, and GPT results, consult the lot-specific Certificate of Analysis (CoA) and product Specification Sheet.
  • If application demands animal‑component‑free or compendial conformance (e.g., USP/EP), verify suitability explicitly on the CoA/Spec Sheet; this listing does not assert such status.

Reaction & Applications

In the context of laboratory workflows, Brain Infusion Powder serves as a nutrient source for microbiological culture rather than a reagent for chemical transformations.

• Microbiological applications (general/literature):

  • Base component for preparing infusion‑type and enrichment media that support growth of fastidious heterotrophs (e.g., organisms that benefit from peptides, amino acids, and growth factors in tissue infusions).
  • Commonly used to prepare broths or agars for isolation, cultivation, and recovery from environmental or industrial samples; also suited to pre‑enrichment before downstream analyses.
  • Can be combined with additional components (e.g., peptones, carbohydrates, buffering salts) per validated recipes to tailor selectivity and growth profiles.

• Practical tips (general):

  • Dissolve the measured powder in high‑purity water with gentle heating and stirring; adjust volume after dissolution.
  • Adjust pH according to the complete formulation requirements. Add heat‑labile supplements aseptically after sterilization.
  • Clarify as needed by low‑speed centrifugation or decanting prior to sterilization to remove coarse particulates (optional, per SOPs).

• Not a chemical reaction reagent:

  • The product is not typically used in organic synthesis or named organic reactions. For chemical transformations, refer to appropriate solvents/reagents instead.

Refer to compendial or institutional SOPs for organism‑specific formulations and incubation parameters. This listing makes no clinical or diagnostic claims; for research use only (per Product Data).

Reaction Conditions

As a media component rather than a chemical reagent, Brain Infusion Powder is used under culture preparation conditions rather than reaction conditions.

• Media preparation parameters (general/literature; follow your validated SOP):

  • Dissolution: Add the measured mass to high‑purity water with stirring; mild heat can aid dissolution. Bring to final volume after solids are dissolved.
  • pH: Adjust to the near‑neutral target specified by your chosen formulation. Final pH after sterilization can shift slightly; confirm post‑autoclave.
  • Sterilization: Autoclave per validated cycle (e.g., gravity or liquid cycle). Add heat‑labile supplements aseptically post‑sterilization via sterile filtration.
  • Incubation: Temperature, atmosphere, and time are organism‑ and method‑dependent. Refer to relevant methods (institutional SOPs/compendial references).

• Performance verification:

  • Use growth promotion testing (GPT) with appropriate strains as required by your quality system to verify lot and batch performance.

Note: Specific masses, pH targets, and sterilization cycles are method‑dependent and not specified for this item; refer to the product CoA/Spec Sheet and your validated procedures.

Safety & Handling

• Hazard information (Product Data):

  • Signal Word: Not specified for this item; refer to SDS.
  • H-Statements: Not specified for this item; refer to SDS.
  • GHS Classification/Pictograms: Not specified for this item; refer to SDS.

• General laboratory safety (always defer to SDS):

  • PPE: Lab coat, safety glasses, and appropriate gloves (e.g., nitrile). Avoid dust generation and inhalation; use local exhaust or a fume hood when handling powders.
  • Incompatibilities: Avoid strong oxidizing agents. Keep away from sources of ignition for dust control. Prevent moisture exposure to avoid caking and possible microbial contamination of the container.
  • First aid (overview): If inhaled, move to fresh air; if on skin/eyes, rinse with water; if ingested, rinse mouth. Seek medical attention as appropriate. Consult SDS for full guidance.

• Microbiological risk considerations (general):

  • The dry powder itself is nonviable; however, once reconstituted and inoculated, it can support robust microbial growth. Treat all used media, spills, and cultures as biohazardous.
  • Decontamination: Autoclave liquid/solid waste at validated time/temperature (e.g., 121 °C) before disposal in accordance with institutional and local regulations.

• Handling best practices:

  • Minimize aerosolization; weigh in a clean, dry area. Reseal promptly. Use dedicated scoops to avoid cross‑contamination. Label reconstituted media with preparation date and lot traceability.

Always consult the product’s SDS and your institution’s biosafety policies for authoritative safety requirements.

Solvent Selection

This product is not a solvent; it is a dry nutrient mixture intended to be dissolved in water to make culture media.

• Practical guidance (general/literature):

  • Primary solvent: High‑purity water (e.g., distilled, deionized, or RO‑DI). Using freshly produced, low‑TOC water helps minimize background and variability in media performance.
  • Ionic content: Elevated chloride, hardness, or residual disinfectants (chloramine) can influence media performance; use laboratory‑grade water systems and validated SOPs.
  • Sterilization: Autoclaving is commonly used after dissolution; filter sterilization is generally not preferred for complex infusion media due to potential filter fouling and retention of macromolecules, but may be used for heat‑labile supplements added post‑autoclave.

• When to choose vs. alternatives:

  • Select Brain Infusion Powder when a nutrient‑rich, undefined base is desired to support fastidious organism cultivation or recovery.
  • Consider defined media or plant/yeast‑based complex media if component traceability, animal‑free status, or batch uniformity are paramount for the application.

• Small comparison (general):

  • Water: mandatory vehicle for dissolution and sterilization.
  • Organic solvents: not applicable for media preparation; the mixture is not designed to dissolve in organic solvents and such use is not recommended.

Storage & Reconstitution

• Item-specific storage (Product Data):

  • Storage Conditions: Room temperature.
  • Shipped In: Normal.

• General storage guidance (not item‑specific specs; follow CoA/SDS):

  • Keep container tightly closed in a cool, dry place. Protect from humidity to prevent caking and premature microbial contamination. Use desiccant where appropriate. Do not store near strong oxidizers.
  • Label container with open date; follow first‑in/first‑out practices. Shelf life and retest date: Not specified for this item; refer to CoA/Spec Sheet.

• Reconstitution/use (general/literature):

  • Dissolve the required mass in high‑purity water according to your validated formulation. Mix until fully dispersed; adjust pH as required by the complete recipe. Sterilize (typically by autoclaving) unless otherwise specified. Add heat‑labile supplements aseptically after sterilization.
  • Storage of prepared media: If not used immediately, store at 2–8 °C protected from light per institutional SOPs. Inspect for turbidity, precipitation, color change, or contamination before use.

• Stability notes:

  • Repeated heating/cooling can promote degradation and caramelization; prepare fresh as needed. Avoid repeated freeze‑thaw of reconstituted media.

For any lot‑specific recommendations (e.g., reconstitution ranges, GPT performance criteria), consult the CoA/Spec Sheet.

Structure & Identity

Brain Infusion Powder is a complex, undefined nutrient mixture used to formulate rich microbiological media. It is not a single chemical entity and therefore has no unique molecular structure.

• Item-specific identifiers (Product Data):

  • CAS: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular Formula: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.
  • InChIKey: Not applicable (complex mixture).
  • SMILES: Not applicable (complex mixture).

• Structural description (general/literature):

  • Heterogeneous blend of low‑molecular‑weight peptides, free amino acids, small metabolites (e.g., nucleotides), minerals, and other water‑extractable components derived from animal tissue infusion used historically in rich media bases.
  • No defined stereochemistry or discrete ring system can be assigned due to mixture composition.

• Functional characteristics (general):

  • Provides nitrogen (peptides/amino acids), carbon (small organics), vitamins/cofactors, and growth factors supporting fastidious microbial growth.
  • Typically used as a component in “infusion” or “enrichment” media formulations rather than as a stand‑alone defined reagent.

Note: As an undefined biological mixture, batch-to-batch composition is controlled via manufacturing specifications and performance criteria rather than a single molecular identity.

Synthetic Utility

This product is not a synthetic organic reagent. It has no defined role in classical chemical synthesis or named organic reactions.

• Laboratory utility (general):

  • Serves as a complex nutrient source to formulate microbiological media for growing and maintaining microbial cultures prior to chemical/biochemical analyses.
  • May be used to generate biomass for downstream biotransformations or enzyme production in research contexts, but it does not participate as a reactant or catalyst.

• If your objective is chemical synthesis:

  • Select appropriate pure reagents and solvents with defined structures and specifications. Brain Infusion Powder should not be used to influence reaction mechanisms, catalysis, or stoichiometry in chemical transformations.

Target Specificity

Not applicable. This product is not an antibody, enzyme, or probe and does not possess target specificity in the immunochemical sense. No antigen/epitope, clone, isotype, or species reactivity information is provided in the Product Data.