BrdU Mouse mAb - Primary antibody, IgG1

Cat. No.: Ab169026
AVAILABLE TO ORDER
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. See COA
Application
IF/ICC
Host species
Mouse
Reactive species
Species independent
Isotype
Mouse IgG1
Clone Number
AB02/5B2
Conjugation
Unconjugated
★
Size
Germany (EU)
USA*
Price
Qty
10μl
Ab169026-10μl
Made to order · 8–12 wks

€50.24

€60.65
Save €10.41 (17.17%)
20μl
Ab169026-20μl
Made to order · 8–12 wks
€86.69
50μl
Ab169026-50μl
Made to order · 8–12 wks
€173.46
100μl
Ab169026-100μl
Made to order · 8–12 wks
€260.23
1ml
Ab169026-1ml
Made to order · 8–12 wks
€1,388.30
Enter a quantity for the sizes you want to add.
🧪

Why this grade

Carrier Free,ExactAb™,Azide Free,Validated,PBS Only,See COA Azide Free,Carrier Free,ExactAb™,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Application
ICC/IF: 0.5μg/mL
Mouse anti BrdU antibody, clone 5B2, is suitable for detecting incorporated BrdU in a wide variety of cell types.

Specifications

Product Name
BrdU Mouse mAb - Primary antibody, IgG1
Synonyms
Bromodeoxyuridine antibody | BrdU antibody | Anti-BrdU antibody | 5-bromo-2-deoxyuridine antibody | BrdU Monoclonal Antibody | Anti-BrdU/5-BrdU Antibody | Anti BrdU
Specifications & Purity
Carrier Free,ExactAb™,Azide Free,Validated,PBS Only,See COA
Host species
Mouse
Immunogen
Bromodeoxyuridine
Positive Control
ICC/IF: HeLa cells
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, PBS Only, Validated
Isotype
IgG1
Light Chain Type
lambda
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Form
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at +4°C short term (1-2 weeks). Store at -20°C. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images

BrdU Mouse mAb (Ab169026) - ICC/IF
IF analysis of HeLa cells labeling BrdU with BrdU Mouse mAb (Ab169026) at 0.5 μg/mL. The cells were fixed with 100% methanol for 5 minutes and then subjected to acid hydrolysis using 2M HCl for 30 minutes at room temperature to denature the DNA. They were then blocked with 2% BSA in PBS for 1 hour and incubated overnight at 4℃ with BrdU Mouse mAb (Ab169026) at 0.5 μg/mL.

 
beta Tubulin Mouse mAb (Ab155823) (FITC) (shown in green) counterstained with the cells after Goat Anti-Mouse Cy5 (Ab156257) (shown in red) incubation. Nuclear DNA was labelled with DAPI (shown in blue).

 
Images were taken on the confocal laser scanning microscope.

Application
ApplicationDilution info
IF/ICC

0.5 μg/mL

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

1 results found

Lot NumberCertificate TypeDateItem
ZJ23R0900105Certificate of AnalysisSep 19, 2023 Ab169026
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

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