Calcein, AM - for fluorescence analysis, ≥96%(HPLC) , CAS No.148504-34-1

Name: Calcein, AM - for fluorescence analysis, ≥96%(HPLC) , CAS No.148504-34-1
SKU: C131116
Availability: InStock

CAS: 148504-34-1 Cat. No.: C131116 Formula: C₄₆H₄₆N₂O₂₃ Molecular Weight: 994.87 EC Number: 200-664-3

129 Citations COA

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Calcein, AM - for fluorescence analysis, ≥96%(HPLC) CAS No(148504-34-1) by Aladdin Scientific

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AVAILABLE TO ORDER

GRADE & PURITY for Fluorescence analysis ? ≥96%(HPLC)

Synonyms

AC1L916S | acetoxymethyl 2-[[2-(acetoxymethoxy)-2-oxo-ethyl]-[[3',6'-diacetoxy-7'-[[bis[2-(acetoxymethoxy)-2-oxo-ethyl]amino]methyl]-3-oxo-spiro[isobenzofuran-1,9'-xanthene]-2'-yl]methyl]amino]acetate | Tetrakis(acetoxymethyl) 2,2',2'',2'''-(((3',6'-diace

Storage

Store at -20°C,Argon charged

Shipped In

Ice chest + Ice pads

Overview Specifications Names and Identifiers Documentation Advanced Data Taxonomic Classification 3D Structure Associated Targets(Human) Mechanisms of Action Certificates（CoA,COO,BSE/TSE and Analysis Chart) Chemical and Physical Properties Documents & Articles Citations of This Product Solution Calculators Reviews

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Why this grade

for fluorescence analysis, ≥96%(HPLC) for Fluorescence analysis for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Store at -20°C,Argon charged Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

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Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

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Literature proof

Cited in 129 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Calcein-AM is a hydrophobic non-fluorescent probe that can permeate the plasma membrane and can be hydrolyzed to Calcein , which is an extremely fluorescent and negatively charged molecule. The probe can be used to stain living cells and has been observed to fluoresce bright green in cytoplasmic Schwann cells. Calcein-AM has been reported to be a neutral substrate for Mdr (P-glycoprotein (Pgp) and multidrug resistance protein (MRP)) and has been used in flow cytometry studies to analyze the function of P-gp and MRP. Studies suggest that Calcein-AM can be used to detect MDR mediated resistance and to be transported by PGP from the plasma membrane. Mitochondrial permeability research has utilized Calcein as a cytosolic fluorophore. Has been used as a cytosolic fluorophore in mitochondrial permeability studies to image pore transition.
A non-fluorescent, membrane permeable probe that can be hydrolyzed to a fluorescent molecule

Specifications

Synonyms

Specifications & Purity

for fluorescence analysis, ≥96%(HPLC)

Storage

Store at -20°C,Argon charged

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

for Fluorescence analysis

Note

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. For more information on AM esters please visit our AM esters FAQ page . Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

Purity

≥96%(HPLC)

Names and Identifiers

Pubchem Sid

528776095

Canonical Smiles

CC(=O)OCOC(=O)CN(CC1=CC2=C(C=C1OC(=O)C)OC3=C(C24C5=CC=CC=C5C(=O)O4)C=C(C(=C3)OC(=O)C)CN(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C

IUPAC Name

acetyloxymethyl 2-[[2-(acetyloxymethoxy)-2-oxoethyl]-[[3',6'-diacetyloxy-7'-[[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]methyl]-3-oxospiro[2-benzofuran-1,9'-xanthene]-2'-yl]methyl]amino]acetate

InChIKey

BQRGNLJZBFXNCZ-UHFFFAOYSA-N

INCHI

1S/C46H46N2O23/c1-25(49)60-21-64-41(55)17-47(18-42(56)65-22-61-26(2)50)15-31-11-35-39(13-37(31)68-29(5)53)70-40-14-38(69-30(6)54)32(12-36(40)46(35)34-10-8-7-9-33(34)45(59)71-46)16-48(19-43(57)66-23-62-27(3)51)20-44(58)67-24-63-28(4)52/h7-14H,15-24H2,1-6H3

Isomeric SMILES

CC(=O)OCOC(=O)CN(CC1=CC2=C(C=C1OC(=O)C)OC3=C(C24C5=CC=CC=C5C(=O)O4)C=C(C(=C3)OC(=O)C)CN(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C

Molecular Weight

994.87

Reaxy-Rn

10757520

Reaxys-RN_link_address

https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=10757520&ln=

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Taxonomic Classification

Taxonomy Tree

Kingdom Organic compounds
- Superclass Organoheterocyclic compounds
  - Class Benzopyrans
    - Subclass 1-benzopyrans
      - Level5 Dibenzopyrans
        Level6 Xanthenes

Kingdom	Organic compounds
Superclass	Organoheterocyclic compounds
Class	Benzopyrans
Subclass	1-benzopyrans
Intermediate Tree Nodes	Dibenzopyrans
Direct Parent	Xanthenes
Alternative Parents	Alpha amino acid esters Diarylethers Phthalides Benzofuranones Isobenzofurans Acylals Aralkylamines Benzenoids Trialkylamines Lactones Oxacyclic compounds Acetals Carbonyl compounds Hydrocarbon derivatives Organic oxides Organopnictogen compounds
Molecular Framework	Aromatic heteropolycyclic compounds
Substituents	Xanthene - Alpha-amino acid ester - Diaryl ether - Benzofuranone - Isobenzofuranone - Alpha-amino acid or derivatives - Phthalide - Isocoumaran - Isobenzofuran - Acylal - Aralkylamine - Benzenoid - Carboxylic acid ester - Lactone - Tertiary aliphatic amine - Tertiary amine - Amino acid or derivatives - Acetal - Carboxylic acid derivative - Oxacycle - Ether - Hydrocarbon derivative - Organic nitrogen compound - Carbonyl group - Organic oxide - Organopnictogen compound - Organic oxygen compound - Amine - Organooxygen compound - Organonitrogen compound - Aromatic heteropolycyclic compound
Description	This compound belongs to the class of organic compounds known as xanthenes. These are polycyclic aromatic compounds containing a xanthene moiety, which consists of two benzene rings joined to each other by a pyran ring.
External Descriptors	organooxygen compound
1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61. DOI: 10.1186/s13321-016-0174-y

3D Structure

Associated Targets(Human)

A498 (42825 Activities)

Lot Number	Certificate Type	Date	Item
D2613215	Certificate of Analysis	Apr 01, 2026	C131116
D2613218	Certificate of Analysis	Apr 01, 2026	C131116
F2208195	Certificate of Analysis	Dec 12, 2025	C131116
H2520002	Certificate of Analysis	Jul 24, 2025	C131116
H2520003	Certificate of Analysis	Jul 24, 2025	C131116
H2520004	Certificate of Analysis	Jul 24, 2025	C131116
I2321025	Certificate of Analysis	Jul 10, 2025	C131116
J2215198	Certificate of Analysis	Jul 18, 2024	C131116
F2208205	Certificate of Analysis	Mar 20, 2024	C131116
I2116116	Certificate of Analysis	Jul 07, 2023	C131116
J2215196	Certificate of Analysis	Oct 20, 2022	C131116

Sensitivity	light sensitive;Moisture sensitive
Molecular Weight	994.900 g/mol
XLogP3	2.900
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	25
Rotatable Bond Count	32
Exact Mass	994.249 Da
Monoisotopic Mass	994.249 Da
Topological Polar Surface Area	305.000 Å²
Heavy Atom Count	71
Formal Charge	0
Complexity	1810.000
Isotope Atom Count	0
Defined Atom Stereocenter Count	0
Undefined Atom Stereocenter Count	0
Defined Bond Stereocenter Count	0
Undefined Bond Stereocenter Count	0
The total count of all stereochemical bonds	0
Covalently-Bonded Unit Count	1

Calcein, AM - for fluorescence analysis, ≥96%(HPLC) , CAS No.148504-34-1

Storage & shipping

Quality documents

Literature proof

Overview

Specifications

Documentation

📋 Safety Data Sheet (SDS)

✅ Certificate of Analysis (COA)

📊 Datasheet

🔬 Specification Sheet

Advanced Data

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Customer Reviews

📚 Citations by Application

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