GRADE & PURITYExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.5 μL/test
Mouse anti Human/Monkey CD20 Antibody, Monoclonal (2H7), could be used for Flow Cytometry.
Specifications
Product Name
CD20 Mouse mAb (AF488) - Primary antibody, specific to CD20
Synonyms
FMC7|B-lymphocyte antigen CD20|B-lymphocyte cell-surface antigen B1|B-lymphocyte surface antigen B1|Bp35|Bp35MGC3969|CD20 antigen|CD20 receptor|CD20|LEU-16|Leukocyte surface antigen Leu-16|Ly-44|Membrane-spanning 4-domains subfamily A member 1|membrane-spanning 4-domains, subfamily A, member 1|S7|APY antibody|ATOPY antibody|B lymphocyte antigen CD20 antibody|B Lymphocyte Cell Surface Antigen B1 antibody|B-lymphocyte cell-surface antigen B1 antibody|B-lymphocyte surface antigen B1 antibody|B1 antibody|Bp 35 antibody|Bp35 antibody|CD 20 antibody|CD20 antibody|CD20 antigen antibody|CD20 receptor antibody|CVID 5 antibody|CVID5 antibody|Fc epsilon receptor I beta chain antibody|Fc Fragment of IgE high affinity I receptor for beta polypeptide antibody|FCER1B antibody|High affinity immunoglobulin epsilon receptor subunit beta antibody|IgE Fc receptor subunit beta antibody|IGEL antibody|IGER antibody|IGHER antibody|LEU 16 antibody|Leu-16 antibody|LEU16 antibody|leukocyte surface antigen Leu 16 antibody|Leukocyte surface antigen Leu-16 antibody|Ly44 antibody|Membrane spanning 4 domains A1 antibody|Membrane spanning 4 domains subfamily A member 2 antibody|membrane-spanning 4-domains A1 antibody|Membrane-spanning 4-domains subfamily A member 1 antibody|MGC3969 antibody|MS4A1 antibody|MS4A2 antibody|S7 antibody
Specifications & Purity
ExactAb™, Validated, 5 μL/test
Biochemical and Physiological Mechanisms
This protein may be involved in the regulation of B-cell activation and proliferation.
Host species
Mouse
Specificity
CD20
Immunogen
Human tonsillar B cells
Positive Control
Flow: Human peripheral blood lymphocytes
Conjugation
AF488
Excitation (nm)
Blue Laser(488nm)
Emission(nm)
525nm
Ex/Em(nm)
Ex:490nm;Em:525nm
Grade
ExactAb™, Validated
Light Chain Type
kappa
Purification Method
Protein A purified
Concentration
5 μL/test
Product Line
Biological Products
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images
CD20 Mouse mAb (AF488) (Ab191694) - Flow Cytometry Flow cytometry analysis of CD20 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD20 Mouse mAb (AF488) (Ab191694) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF488) (Ab190245).
CD20 Mouse mAb (AF488) (Ab191694) - Flow Cytometry Flow cytometry analysis of CD20 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with CD20 Mouse mAb (AF488) (Ab191694) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF488) (Ab190245). Black - Unlabelled control, cells without incubation with primary antibody.
Application
Application
Dilution info
Flow Cytometry
5μl/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Reviews
Customer Reviews
Frequently Asked Questions
How should this product be stored?
Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Validated mean?
Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
How is this product shipped?
This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD20 Mouse mAb (AF488)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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