CD20 Mouse mAb - Primary antibody, specific to CD20, Mouse IgG2b

Cat. No.: Ab094175
AVAILABLE TO ORDER
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. ≥95%(SDS-PAGE) See COA
Application
Animal Model, Depletion, Flow Cytometry, Functional Assay, IHC, IP
Host species
Mouse
Reactive species
Human, Monkey
Isotype
Mouse IgG2b
Clone Number
2H7
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μg
Ab094175-10μg
10
$29.90
50μg
Ab094175-50μg
4
$79.90
100μg
Ab094175-100μg
2
$121.90
1mg
Ab094175-1mg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$739.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

Carrier Free,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA Azide Free,Carrier Free,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Mouse anti Human/Monkey CD20 Antibody, Monoclonal (2H7), could be used for Flow, IHC, IP, in vivo B cell depletion in hCD20 Tg mice and so on.
Application:
Flow: Use at an assay dependent concentration.
IHC: Use at an assay dependent concentration.
IP: Use at an assay dependent concentration.
in vivo B cell depletion in hCD20 Tg mice: Use at an assay dependent concentration.
Protein Function
This protein may be involved in the regulation of B-cell activation and proliferation.

Specifications

Product Name
CD20 Mouse mAb - Primary antibody, specific to CD20, Mouse IgG2b
Synonyms
FMC7 | B1 | B-lymphocyte antigen CD20 | B-lymphocyte cell-surface antigen B1 | B-lymphocyte surface antigen B1 | Bp35 | Bp35MGC3969 | CD20 antigen | CD20 receptor | CD20 | CD20S7 | CVID5 | LEU-16 | Leukocyte surface antigen Leu-16 | Ly-44 | Membrane-spann
Specifications & Purity
Carrier Free, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA
Host species
Mouse
Specificity
CD20
Immunogen
Human tonsillar B cells
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, PBS Only, Validated
Isotype
Mouse IgG2b
Light Chain Type
kappa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at 2-8°C,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.
Images

CD20 Mouse mAb (Ab094175) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD20 with CD20 Mouse mAb (Ab094175) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Mouse IgG Isotype Control Antibody (Biotin) (Ab180306) (left panel) and detected with SA-PE (rp156150).

CD20 Mouse mAb (Ab094175) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD20 (red) with CD20 Mouse mAb (Ab094175) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

CD20 Mouse mAb (Ab094175) - Flow Cytometry
Flow Cytometry analysis of Raji cells labelling CD20 (red) with CD20 Mouse mAb (Ab094175) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Application
ApplicationDilution info
IP

Use at an assay dependent concentration.

IHC

Use at an assay dependent concentration.

Flow Cytometry

Use at an assay dependent concentration.

in vivo B cell depletion in hCD20 Tg mice

Use at an assay dependent concentration.

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
MS4A1 Tclin B-lymphocyte antigen CD20 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Mechanisms of Action
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0707926Certificate of AnalysisJul 08, 2024 Ab094175
ZJ24F0707925Certificate of AnalysisJul 08, 2024 Ab094175
ZJ24F0707924Certificate of AnalysisJul 08, 2024 Ab094175
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Carrier Free grade guide → View Validated grade guide → View Azide Free grade guide → View PBS Only grade guide →

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