Application:
Flow: 5μL/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume.
CD25 Rat mAb (APC) (Ab179012) - Flow Cytometry
BALB/c mouse splenocytes are stained with CD25 Rat mAb (APC) (Ab179012) and CD4 Rat mAb (FITC) (Ab181111) (right). BALB/c mouse splenocytes are stained with CD4 Rat mAb (FITC) (Ab181111) and Rat IgG Isotype Control Antibody (APC) (Ab180313) (left).
CD25 Rat mAb (APC) (Ab179012) - Flow Cytometry
Con A-stimulated (1 day) BALB/c mouse splenocytes (right panel) stained with CD25 Rat mAb (APC) (Ab179012) (red) compared to an isotype control (blue). Blue - Rat IgG Isotype Control Antibody (APC) (Ab180313). Black - Unlabelled control, cells without incubation with primary antibody. Untreated BALB/c mouse splenocytes were used as control (left panel).
5μl/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0404891 | Certificate of Analysis | Aug 03, 2026 | Ab179012 |
| ZJ24F0404892 | Certificate of Analysis | Aug 03, 2026 | Ab179012 |
| ZJ24F0404893 | Certificate of Analysis | Aug 03, 2026 | Ab179012 |
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View Ex:650nm grade guide → View Em:660nm grade guide →
