Coomassie Blue Fast Staining Solution - BioReagent, for PAGE, for western blot

Cat. No.: C751629

GRADE & PURITY BioReagent, for PAGE, for Western blot

Storage

Protected from light,Room temperature

Shipped In

Normal

Application

PAGE, WB

Overview

Coomassie Blue Fast Staining Solution is an improved protein gel stain formulated with Coomassie Brilliant Blue G250. It features rapid staining, high sensitivity and safety. Satisfactory staining results can be obtained within 3 to 60 minutes. This non-toxic formula contains no corrosive, volatile strong acids or organic reagents.

Instructions for Use

1. After electrophoresis, take out the PAGE gel and immerse it in sufficient CFAS protein gel staining solution to fully cover the gel.

2. Stain for 3 to 60 minutes at room temperature with gentle shaking on a horizontal shaker.

3. Terminate staining when the desired color intensity is achieved. Rinse the gel with clean water or destaining solution, then capture imaging.

Protein Content	>1μg	100ng-1μg	10ng-100ng
Incubation Time	3-15min	10-30min	30-60min

Precautions

1. Gel washing is unnecessary and not recommended before staining.

2. Ethanol or acetic acid fixation is not required prior to use.

3. The staining solution can be reused 2 to 3 times. Prolong staining duration appropriately for reused solution.

4. Wear lab coat and gloves for personal protection during operation.

Specifications

Specifications & Purity

BioReagent, for PAGE, for western blot

Stability And Storage

Store at room temperature long term (12 months). Store in the dark.

Storage

Protected from light, Room temperature

Shipped In

Normal

Grade

BioReagent, for PAGE, for Western blot

Chemical and Physical Properties

• Sensitivity: Light-sensitive

References

1. Yiming Liu, Mingrui Zhang, Jingjing Zhao, Yongshuo Ren, Shubin Li, Weichen Wang, Wei Mu, Xiaojun Han.  (2025)  Construction of a De Novo Nucleotide Biosynthesis Pathway in Artificial Cells for RNA Transcription.  Journal of the American Chemical Society,      [PMID:40708544] [10.1021/jacs.5c09375]