CYCA-117-70 , CAS No.1958262-04-8

CAS: 1958262-04-8 Cat. No.: C1449875 Formula: C₁₉H₂₄FN₅O Molecular Weight: 357.43

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

CYCA-117-70 is a DCAF1 ligand (K D : 70 μM). CYCA-117-70 is an ideal chemical handles for PROTACs recruiting DCAF1.

Specifications

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Names and Identifiers

Molecular Weight

357.43

Application Protocols

• Item-specific status
  • No tested application protocols are provided for CYCA-117-70 in the Product Data.
• General protocols for small-molecule screening (not item-specific)
  • DMSO stock preparation
    • Equilibrate vial to room temperature in a desiccator. Add anhydrous DMSO to prepare a 10–50 mM stock. Vortex and, if needed, sonicate briefly. Record exact concentration by weight or UV if the extinction coefficient is known (if not, gravimetric preparation is preferred).
    • Filter through a 0.22 µm PTFE filter if particulates persist. Aliquot into low-bind tubes under dry N2/Ar to minimize moisture uptake.
  • Aqueous dilution for assays
    • Add DMSO stock slowly into pre-warmed, vigorously mixed buffer/culture media to reach the desired final concentration while keeping DMSO constant (typically ≤0.5–1% v/v). Inspect for precipitation; if present, reduce concentration or adjust co-solvent.
  • Plate-based screening
    • Use acoustic or pintool dispensing for low-volume transfers. Include vehicle controls, positive controls (mechanism-relevant), and orthogonal readouts to detect interference (e.g., auto-fluorescence, redox cycling).
  • Stability sampling
    • For time-course studies, sample immediately after dosing and at endpoint; analyze by LC–MS to confirm exposure consistency.
• Reporting
  • Document lot number, preparation date, solvent composition, and storage conditions with your assay data for traceability.

Biological Roles

• Item-specific status
  • No target, pathway, or mechanism-of-action information is provided for CYCA-117-70. It is supplied for research use only as part of a small-molecule/compound library.
• General considerations for library compounds (not item-specific)
  • Potential roles are typically explored empirically through phenotypic screens or target-based assays, followed by orthogonal confirmation (biophysical binding, CETSA/DSF, pulldown) and preliminary structure–activity relationships if analogs are available.
  • Off-target assessment may include panel screening, cytotoxicity baselines, and early ADME profiling (stability in microsomes/S9, permeability, protein binding) to contextualize biological readouts.
  • Chemical liabilities (PAINS, aggregators, redox cyclers) should be evaluated using curated filters and counter-screens to avoid artifactual signals.
• Documentation and transparency
  • Absent item-specific data, report full assay conditions, vehicle percentages, and control compounds to enable interpretation and replication.
• Compliance note
  • For research use only. Not intended for diagnostic or therapeutic applications.

Buffer Applications

• Applicability
  • CYCA-117-70 is a small organic compound without disclosed acid–base properties; it is not intended as a buffering agent. No pKa or buffer-capable functional groups are provided in the Product Data.
• General lab guidance (not item-specific)
  • If introducing this compound into buffered systems, choose buffers based on biological assay needs (e.g., PBS pH 7.4, HEPES pH 6.8–8.2, Tris pH 7–9) and confirm compound solubility/compatibility at the working pH.
  • Add from a concentrated organic stock (commonly DMSO) into the buffer under agitation to avoid local supersaturation and precipitation. Keep the final organic co-solvent level constant across controls and treatments.
• Action item
  • Because no acid/base data are available, do not rely on this compound to maintain pH; select an appropriate buffer system independently.

Green Alternatives

• Context
  • The structure and solvent preferences for CYCA-117-70 are not specified; therefore, green alternative guidance is presented generally for dissolving and handling small-molecule screening compounds.
• Greener solvent considerations (general)
  • Prefer bio-based or lower-toxicity solvents where feasible: 2-MeTHF, CPME, ethyl acetate, propylene carbonate, and short-chain alcohols can sometimes replace chlorinated or high-boiling dipolar aprotics.
  • For assay stocks, DMSO is common; consider propylene glycol or ethanol for specific biological systems if solubility and tolerance permit, noting their distinct toxicity and compatibility profiles.
• Trade-offs
  • 2-MeTHF/CPME: improved safety and life-cycle metrics vs THF/Et2O; however, solvency for polar/ionic motifs is lower than DMSO/DMF.
  • Ethyl acetate and alcohols: greener and volatile; limited capability for very hydrophobic or strongly hydrogen-bonding solutes.
  • Propylene carbonate: low volatility and good polarity; viscosity and miscibility constraints apply.
• Practical approach
  • Run a micro-solvent screen starting with greener options; escalate to less preferred solvents only if required for solubility/stability.
  • Minimize DMSO concentration in biological assays (≤0.5–1% v/v) and implement solvent back-extractions to reduce organic solvent loads in workups.
  • Apply solvent recycling where permitted and segregate halogenated waste streams to reduce disposal impact.
• Note
  • No item-specific green metrics (EHS scores, life-cycle data) are available for this product.

Pharmaceutical Uses

• Item-specific status
  • No pharmacopeial status, excipient role, or formulation use is provided for CYCA-117-70. The product is labeled For research use only and is not intended for human or veterinary use.
• General context (not item-specific)
  • Small molecules from screening libraries may serve as early tool compounds in discovery research. If a compound advances, pre-formulation studies typically assess solubility, solid form (polymorph/salt), and stability to guide later development.
  • Without grade, impurity profile, or GMP documentation, materials of this type should not be incorporated into clinical or manufacturing workflows.
• Practical guidance
  • If used in dosage-form research models (in vitro only), document solvent vehicles (e.g., DMSO, PEG400, Captisol) and ensure vehicle controls are included to dissociate vehicle effects from compound responses.

Physical Properties

• Item-specific (from Product Data)
  • Appearance: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• Literature/computed values
  • No structure has been provided; therefore, literature or computed properties (bp, mp, density, pKa, logP, refractive index) cannot be established for this item without the molecular structure.
• Solubility and handling expectations (general guidance for small-molecule library members; not item-specific)
  • Many screening compounds are supplied as solids or films intended for dissolution in anhydrous DMSO to 10–50 mM stock solutions. Actual solubility limits vary widely by chemotype.
  • Water solubility is often limited for hydrophobic scaffolds; co-solvent strategies (DMSO into buffer with vigorous mixing) are commonly required for biological assay introduction.
• Thermal behavior (general, not item-specific)
  • Melting point and thermal stability are unknown for this item. Avoid prolonged heating until specifications are confirmed.
• UV/Vis characteristics
  • UV cutoff or extinction coefficients are not available for this item; do not assume chromatographic transparency at low wavelengths. Validate detector settings empirically when using HPLC/UPLC.
• Action items
  • For lot-specific properties, consult the CoA/Spec Sheet. If absent, determine practical solubility via a small-sample titration into DMSO, then water/buffer, documenting concentration at first persistent turbidity.

Quality & Grades

• Item-specific (from Product Data)
  • Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.
• Understanding typical offerings for library compounds (general context)
  • Screening compounds are commonly supplied at research or screening grade, targeting purity suitable for biochemical/biophysical assays (often ≥95% by HPLC), but exact thresholds vary by supplier and product. Since no grade/purity is specified here, rely on the CoA for definitive acceptance criteria.
  • Identity is typically supported by LC–MS and/or NMR. If the CoA is unavailable, consider performing in-house QC prior to critical experiments (orthogonal LC methods, accurate-mass confirmation, and, when relevant, chiral analysis).
  • Residual solvent, water content (Karl Fischer), and inorganic impurities (metals) are not specified for this item; consult the CoA if such limits are critical for your use (e.g., catalysis or sensitive biophysical assays).
• Stabilizers and additives
  • No stabilizer information is provided. If long-term storage is planned, aliquot under inert atmosphere and desiccation to minimize hydrolysis/oxidation risks unless the structure indicates otherwise.
• Traceability and documentation
  • Record SKU (C1449875), CAS (1958262-04-8), lot/batch, and receipt condition. Attach the CoA and any in-house QC to your ELN entry for reproducibility.
• Practical tip
  • If analytical UV transparency is important (e.g., HPLC at 210–220 nm), verify blank baselines with your specific solvent system, as UV cutoff is not provided.

Reaction & Applications

• Item-specific applicability
  • CYCA-117-70 is listed as part of a small-molecule/compound library. No synthetic role, reactive functional groups, or application notes are provided in the Product Data.
• General research uses for library compounds (not item-specific)
  • Biochemical or cell-based screening: evaluation as a tool compound candidate in enzymatic assays, phenotypic screens, or binding studies.
  • Method development: use as an internal reference in LC–MS method qualification when structural data are known.
  • Physicochemical profiling: determination of logD, solubility, permeability, and stability to inform SAR campaigns if the compound becomes a hit.
• If employing in chemical reactions (only after structure is known)
  • Confirm compatibility with reaction media, bases/acids, and temperature profiles. Unknown liabilities (e.g., hydrolyzable esters, oxidizable heterocycles) can lead to decomposition or side reactions.
  • Perform small-scale pilots with in situ monitoring (TLC, LC–MS). Establish quench and workup conditions that preserve the parent compound.
• Documentation
  • Because item-specific reactivity information is not available, capture full experimental context (solvent, time, temperature, atmosphere) for reproducibility and troubleshooting.

Reaction Conditions

• Applicability
  • No structure or reactivity data are provided for CYCA-117-70; item-specific reaction conditions cannot be recommended.
• General guidance if chemical modification is attempted (not item-specific)
  • Conduct micro-scale scouting experiments to determine solvent, base/acid, temperature, and atmosphere compatibility. Monitor by LC–MS to detect degradation vs transformation.
  • Keep temperatures conservative initially (0–40°C) until stability is established. Avoid strong oxidants/reductants without prior assessment.
  • Where functional group reactivity is suspected (e.g., halides, nitriles, amides), consult literature precedents for analogous scaffolds before scale-up.
• Stability checks
  • Evaluate compound integrity under light, oxygen, and moisture. Use amberware/inert gas purges when needed. Confirm post-reaction purity and mass balance.

Safety & Handling

• Item-specific (from Product Data)
  • GHS classification: Not specified for this item; refer to SDS.
  • Signal word / Pictograms / H-statements: Not specified for this item; refer to SDS.
  • Storage conditions: Store at −20°C.
  • Shipping: Ice chest + ice pads.
• General laboratory precautions (not item-specific; follow your institutional EHS and the SDS once obtained)
  • Handle in a chemical fume hood with appropriate PPE: lab coat, safety glasses, and chemically resistant gloves (e.g., nitrile). Avoid inhalation of dust/aerosols and contact with skin/eyes.
  • Prevent accidental ingestion; do not mouth-pipette. Wash hands thoroughly after handling.
  • Avoid incompatible reagents until reactivity is known. As a conservative baseline, segregate from strong oxidizers, strong acids/bases, and reducing agents.
  • If supplied as a fine powder, minimize dust generation; use antistatic measures when appropriate.
• First-aid overview (defer to SDS for authoritative instructions)
  • Inhalation: Move to fresh air; seek medical attention if symptoms persist.
  • Skin contact: Wash with soap and water; remove contaminated clothing.
  • Eye contact: Rinse cautiously with water for several minutes; remove contact lenses if present and easy; seek medical advice for persistent irritation.
  • Ingestion: Rinse mouth; seek medical attention. Do not induce vomiting unless directed by medical personnel.
• Spill/fire response (general)
  • Small spills: Absorb with inert material (e.g., vermiculite), place in chemical waste. Avoid dust formation.
  • Fire: Use CO2, dry chemical, or foam extinguishers as appropriate. Combustion products are unknown; wear self-contained breathing apparatus in case of major fire.
• For research use only. Consult the SDS for the specific lot before scale-up or in vivo work.

Solvent Selection

• Item-specific limitation
  • The structure and polarity class for CYCA-117-70 are not provided; no item-specific solvent recommendations can be made. Refer to the CoA or conduct a micro-solubility screen.
• General strategy for small-molecule screening compounds (not item-specific)
  • Primary stock solvent: Anhydrous DMSO is the default for diverse chemotypes due to broad solubilizing power and assay compatibility at ≤1% v/v.
  • Secondary options: DMF or NMP can improve solubility for highly lipophilic scaffolds; ethanol, isopropanol, or acetonitrile may suffice for moderately polar compounds. Water buffers are suitable only after confirming aqueous solubility.
  • Aqueous introduction: Add DMSO stock slowly into vigorously mixed buffer/media; consider inclusion of non-ionic surfactants (e.g., 0.01–0.05% Tween 80) where biologically acceptable to suppress precipitation. Filter through low-protein-binding membranes if particulate forms.
• Small comparison (general)
  • DMSO: very high solvency; biologically tolerated at low %; hygroscopic and can oxidize certain motifs.
  • DMF/NMP: strong solvency; higher cytotoxicity; more challenging to remove; reserve for stubborn cases.
  • ACN/EtOH/iPrOH: volatile, easier to remove; limited for very hydrophobic compounds.
• Practical notes
  • Always verify solubility at the working temperature. Some compounds exhibit inverse temperature solubility.
  • Avoid repeated freeze–thaw of DMSO stocks; aliquot to minimize water pickup and degradation.
  • Document final vehicle composition in assays; keep DMSO constant across controls and treatments.

Storage & Reconstitution

• Item-specific (from Product Data)
  • Storage: Store at −20°C.
  • Shipped: Ice chest + ice pads.
• Reconstitution guidance (general; not item-specific)
  • Allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation. If supplied as a solid/film, dissolve in anhydrous DMSO to a convenient stock concentration (e.g., 10–50 mM). If the structure indicates instability in DMSO (not known here), consider alternative anhydrous solvents (DMF, ACN) validated by a small test.
  • Aliquot stocks into dry, inert-gas-flushed vials or low-bind tubes to avoid repeated freeze–thaw and moisture ingress. Clearly label concentration, solvent, and date.
  • Store aliquots at −20°C or below in a desiccated, dark environment. Avoid frost-free freezers that cycle temperatures frequently. Protect from light if chromophores are suspected; use amber vials as a precaution when uncertain.
  • Thaw on ice or at room temperature as appropriate; mix thoroughly before use. Discard aliquots showing visible precipitation that does not redissolve or evidence of degradation by LC–MS/UPLC.
• Stability
  • Solution and solid-state stability are not specified for this item; refer to CoA/Spec Sheet. Establish in-house stability with periodic LC–MS checks if long-term storage is planned.
• Compliance
  • For research use only. Not for human or veterinary use.

Structure & Identity

• Item-specific (from Product Data)
  • SKU: C1449875
  • Product name: CYCA-117-70
  • CAS: 1958262-04-8
  • Category: Small molecules and compound libraries (小分子和化合物库)
  • Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
  • SMILES: Not specified for this item; refer to CoA/Spec Sheet.
  • InChIKey: Not specified for this item; refer to CoA/Spec Sheet.
• Interpreted type and structural features
  • This listing denotes a research small molecule supplied for screening/library use. Specific functional groups, ring systems, stereochemistry, and substitution pattern are not provided in the product data.
  • Without a structure, we cannot annotate heteroatoms, hydrogen-bond donors/acceptors, or aromaticity for this item. Please consult the Certificate of Analysis (CoA) or structure sheet associated with the specific lot when available.
• Practical identity guidance
  • If structural confirmation is required in your workflow, common orthogonal checks include 1H/13C NMR, LC–MS (ESI±), HRMS, and, where relevant, chiral HPLC. Match any observed data to the supplier CoA.
  • For cheminformatics registration in ELNs, use the CAS (1958262-04-8) as a temporary key and update with the definitive SMILES/InChIKey when provided.
  • If you received a QR or data matrix on the vial/label, scan to retrieve any extended identity metadata (lot-specific).

Synthetic Utility

• Item-specific limitation
  • The structure and functional groups of CYCA-117-70 are not disclosed in the Product Data; therefore, specific synthetic transformations, protecting-group strategies, or retrosynthetic roles cannot be described for this item.
• General remarks (not item-specific)
  • Library compounds may contain motifs amenable to diversification (e.g., aryl halides for cross-coupling, amines/acids for amide coupling). Confirmation requires structural information.
  • If derivatization is desired, obtain the structure and assess functional group compatibility and potential liabilities (peroxides, azides, strained rings) before planning chemistry.
  • For SAR exploration, consider parallel synthesis techniques once the scaffold is known, employing orthogonal analytics (LC–MS, UPLC-UV, NMR) to verify product identity and purity.

Target Specificity

• Item-specific status
  • No biological target, enzyme, receptor, or pathway specificity is provided for CYCA-117-70 in the Product Data.
• General note (not item-specific)
  • In discovery research, target specificity is established through primary assays, secondary orthogonal confirmations, and selectivity panels. Absent such data, treat any observed activity as provisional and verify with counterscreens.
• Action item
  • Refer to any project-specific screening results or contact the supplier if a target annotation becomes available for the lot you received.