Cypermethrin solution - analytical standard, 10ug/ml,in petroleum ether,u=6% , CAS No.52315-07-8

CAS: 52315-07-8 Cat. No.: C109863 Formula: C₂₂H₁₉Cl₂NO₃ Molecular Weight: 416.3 Beilstein Registry Number: 8444118 EC Number: 257-842-9

GRADE & PURITY Analytical standard, 10ug/ml,in petroleum ether,u=6%

Synonyms

Alphamethrine

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads,FedEx DG Service

Specifications

Synonyms

Alphamethrine

Specifications & Purity

analytical standard, 10ug/ml,in petroleum ether,u=6%

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads,FedEx DG Service

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

Analytical standard

Names and Identifiers

Isomeric SMILES

CC1(C(C1C(=O)OC(C#N)C2=CC(=CC=C2)OC3=CC=CC=C3)C=C(Cl)Cl)C

WGK Germany

3

Alternate CAS

86753-92-6

UN Number

3077

Packing Group

III

Molecular Weight

416.3

Beilstein

8444118

Reaxy-Rn

5773157

Reaxys-RN_link_address

https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=5773157&ln=

Chemical and Physical Properties

• Sensitivity: Light sensitive.
• Refractive Index: 1.57
• Flash Point(°C): 100°C
• Melt Point(°C): 60-80°C

Application Protocols

General protocols for using analytical standards of cypermethrin (guidance; adjust per your SOP and CoA)

• Stock verification

  • Record lot number, certified concentration, and solvent from the CoA. Inspect for precipitation or solvent loss; equilibrate to room temperature before opening to avoid condensation.

• Calibration curve preparation (example)

  • Prepare serial dilutions in instrument-compatible solvent (e.g., hexane/iso-octane for GC-ECD/MS; acetonitrile for LC-MS/MS).
  • Typical ranges: 0.5–500 ng/mL for LC-MS/MS; 5–5000 ng/mL for GC-ECD/MS, depending on sensitivity and matrix. Confirm with method requirements.
  • Use amber vials; cap immediately. Include a solvent blank and, if available, an internal standard.

• Matrix-matched calibration

  • Spike blank extracts at multiple levels post-extraction to match matrix effects. Maintain constant organic composition across standards and samples.

• System suitability

  • Inject a mid-level standard to verify retention time window, peak symmetry, resolution of isomer clusters (if applicable), and detector response. Check carryover by a subsequent blank.

• Sample spiking and recovery checks

  • Fortify representative matrices at low/mid/high levels (e.g., 10/50/200 ng/g). Process alongside unknowns to monitor recoveries and precision.

• Stability and handling

  • Minimize light exposure; keep standards on autosampler at 4–10 °C if available. Discard working solutions per validated holding times.

Note: Numerical targets above are literature-style examples; always follow your validated method and the concentration stated on the CoA.

Biological Roles

General biological context (literature; not a statement of use for this product)

• Mode of action: Cypermethrin is a Type II pyrethroid that modulates voltage-gated sodium channels in nerve membranes, prolonging channel opening and causing repetitive firing in susceptible organisms (notably insects). The presence of the α-cyano group differentiates Type II from Type I pyrethroids.
• Selectivity: Higher potency in arthropods versus mammals is associated with differential sodium channel isoforms and metabolic detoxification rates, though mammalian neurotoxicity can occur at sufficient exposure.
• Metabolism: In biota, primary routes include ester hydrolysis to yield the corresponding acid and alcohol, oxidative metabolism (e.g., on the phenoxy ring and isobutyl/cyclopropane moieties), and conjugation. Isomer-specific biotransformation rates are reported.
• Environmental fate: Strong sorption to soils and sediments due to high hydrophobicity (log Kow ~6.6–6.9); low water solubility; susceptible to photolysis and biodegradation; very toxic to aquatic organisms. Bioaccumulation potential exists but may be mitigated by metabolic degradation in higher organisms.

Analytical implications

• Matrix effects: High lipid affinity necessitates robust cleanup for fatty matrices (GPC, C18/Florisil).
• Isomerism: Biological and environmental processes may alter isomer ratios; analytical methods should account for potential isomeric interconversion or selective degradation.

Note: This information provides context for residue analysis and environmental monitoring only. The product is for research use only.

Buffer Applications

Not typically applicable.

• Cypermethrin is a hydrophobic neutral ester and is not used to prepare aqueous buffer systems. For this product, the relevant sections are Solvent Selection, Reaction & Applications (analytical), and Storage & Reconstitution.
• If aqueous phases are present in your method (e.g., LC-MS gradients), cypermethrin is introduced from an organic diluent and eluted under high-organic conditions; buffers are chosen for instrument compatibility rather than for buffering cypermethrin itself.

Green Alternatives

Perspective

• As an analytical standard, the primary opportunities for “greener” practice relate to solvent choice, method design, and waste minimization rather than substituting the analyte.

Greener solvent and method choices (literature-based)

• Prefer lower-toxicity, lower-VOC solvents when compatible with the analytical platform.
• Reduce solvent volumes via microextraction or miniaturized QuEChERS workflows.
• Use multi-residue methods to consolidate runs and cut total solvent consumption.

Comparison of common solvent options for cypermethrin standards (general guidance)

• Hexane/iso-octane: GC-compatible, low polarity; high VOC and flammability; limited “green” credentials.
• Heptane (as alternative to hexane): Similar chromatographic behavior with improved toxicological profile.
• Ethyl acetate: Biobased potential, good solvency; higher polarity may affect GC injection systems; readily biodegradable.
• Acetonitrile: LC-compatible; higher EHS burden in production but excellent performance in multi-residue LC-MS; recycle via solvent recovery when possible.
• 2-MeTHF or cyclopentyl methyl ether (CPME): Greener ethers; may be acceptable for extractions; evaluate instrument compatibility before use as carrier/diluent.

Trade-offs

• Analyte stability is paramount: avoid basic media that accelerate ester hydrolysis. When adopting greener solvents, validate for recovery, peak shape, and detector response.

Waste minimization

• Use pre-mixed multi-analyte standards, sample pooling where permissible, and on-instrument dilution to reduce preparation waste.

Pharmaceutical Uses

Not applicable to this product.

• This item is supplied as an analytical standard for research and laboratory calibration. It is not an excipient or pharmaceutical ingredient and carries no pharmacopeial status in this context.
• Do not use for therapeutic, diagnostic, or clinical applications.

Physical Properties

Item-specific (this lot)

• Solvent, concentration, appearance, and any certified physical constants: Not specified for this item; refer to CoA/Spec Sheet.

General/literature values for neat cypermethrin (reference only; not specifications for this solution)

• Physical state: viscous liquid/low-melting solid, typically colorless to pale yellow (literature)
• Melting point: ~60–80 °C (isomer-mixture dependent; literature)
• Boiling point: decomposes; distillation under high vacuum is not recommended (literature)
• Density: ~1.2 g/mL at 20 °C (literature, mixture-dependent)
• Solubility: very low in water (<10 mg/L at 20–25 °C); readily soluble in nonpolar and moderately polar organics (acetone, ethyl acetate, hexane, toluene, acetonitrile; literature)
• Log Kow (octanol/water): ~6.6–6.9 (highly hydrophobic; literature)
• pKa: not applicable (neutral ester; literature)
• Vapor pressure: very low (~1×10^-7 to 1×10^-5 mmHg at 20–25 °C; literature)
• UV absorption: strong aromatic/π→π* bands; exact cutoffs depend on solvent and isomer mix (literature)

Analytical considerations

• Because cypermethrin is a multi-isomer mixture, observed melting behavior, refractive index, and chromatographic retention may vary with isomer ratio and solvent. For precise calibration (e.g., GC-ECD, GC-MS, LC-MS/MS), always match matrix and solvent to method validation and use the concentration certified on the CoA.

Quality & Grades

• Item-specific grade (from Product Data)

  • Grade: Analytical standard. Supplied as a ready-to-use solution (solvent and concentration: Not specified for this item; refer to CoA/Spec Sheet).

• What “Analytical standard” implies

  • Intended use: calibration, system suitability, and QC for chromatographic and mass spectrometric methods (e.g., GC-ECD, GC-MS, LC-MS/MS) targeting cypermethrin in environmental, food, or industrial matrices.
  • Traceability: Typically supplied with a Certificate of Analysis (CoA) listing certified concentration, uncertainty, and method of value assignment. May include isomer composition, impurity profile, and stability information where applicable.
  • Purity vs. certainty: For standards, the critical parameter is the certified concentration and identity confirmation rather than a single “purity” percentage for the neat material. Matrix-matched and solvent-specific behavior is described on the CoA.

• Stabilizers and additives

  • Not specified for this item; refer to CoA/Spec Sheet. Some pesticide standards employ stabilizers or specific solvents to maximize shelf-life and minimize adsorption and hydrolysis.

• Best practices for analytical standards

  • Minimize freeze–thaw cycles; prepare single-use aliquots.
  • Record opening date and maintain a calibration standard log.
  • Verify performance periodically against a fresh lot or secondary standard as part of ongoing method control.

Note: Do not use this product for any purpose other than research and analytical calibration as indicated.

Reaction & Applications

Primary application domain (for this product)

• Analytical standard for cypermethrin quantification in complex matrices. Typical use cases include:
  • Environmental monitoring: water, sediment, soil, and air particulate extracts.
  • Food safety: fruits, vegetables, grains, oils, and animal-derived products.
  • Industrial hygiene and product QC: coatings, treated materials, and process streams.

Methodological notes (literature-guided; not product specifications)

• Chromatography
  • GC-ECD/GC-MS: Widely used; resolve total cypermethrin and sometimes report cis/trans isomer groups. Programmed temperature GC with mid- to low-polarity columns (e.g., 5% phenyl polysiloxane) is common.
  • LC-MS/MS: Employed when thermal lability or matrix constraints favor LC; multiple reaction monitoring (MRM) transitions target the cyano-containing fragments.
• Sample preparation
  • QuEChERS for foods and plant matrices; adjust cleanup (PSA, C18, GCB) to balance pigment removal and recovery.
  • SPE (C18, HLB) for water; liquid–liquid extraction with hexane/ethyl acetate for oily matrices.
• Calibration strategies
  • Prepare multi-point calibration curves across expected concentration ranges; use internal standards where available (e.g., isotopically labeled pyrethroids) to correct for matrix effects.
  • Consider isomer composition: Some methods quantify as total cypermethrin; others track key isomer pairs. Match the reporting convention to regulatory method.

Practical tips

• Use amber glassware to limit photodegradation; pre-rinse vials to reduce adsorption.
• Verify solvent compatibility with your injection system and liner.
• Store working dilutions cold and use within validated holding times to maintain accuracy.

Reaction Conditions

Not typically applicable for preparative chemistry.

• As an analytical standard, cypermethrin solution is used for calibration and spiking rather than for performing chemical reactions. Consequently, no item-specific reaction conditions apply.

Literature guidance relevant to stability tests

• Hydrolysis: Base-catalyzed ester cleavage is accelerated at elevated pH and temperature; acidic conditions are comparatively milder but can still induce hydrolysis over time.
• Photolysis: UV exposure can cause degradation on aromatic and ester moieties; protect samples with amber glass and minimize light exposure.
• Thermal behavior: Prolonged heating can lead to decomposition; GC analysis is performed with appropriate inlet liners and temperatures validated to prevent on-injector degradation.

If performing forced-degradation studies for method validation, document conditions (pH, solvent, temperature, light intensity) and compare to stability-indicating method criteria.

Safety & Handling

Item-specific hazard data (from Product Data)

• GHS signal word, hazard statements, pictograms, classification: Not specified for this item; refer to the SDS for authoritative details.
• Storage: Store at -20°C. Shipments are sent cold (ice chest + ice pads) via DG service.

General safety information (literature; for guidance only)

• Hazard profile: Cypermethrin is a synthetic pyrethroid; typically harmful if swallowed, in contact with skin, or inhaled; causes skin and eye irritation; very toxic to aquatic life with long-lasting effects. Avoid environmental release.
• PPE: Laboratory coat, nitrile gloves, splash goggles; handle in a fume hood. Avoid skin contact and inhalation of vapors/aerosols.
• Incompatibilities: Strong oxidizers; strong bases and acids may catalyze ester hydrolysis; avoid excessive heat and direct sunlight.
• First aid (summary; defer to SDS)
  • Inhalation: Move to fresh air; seek medical attention if symptoms persist.
  • Skin: Wash with soap and water; remove contaminated clothing.
  • Eyes: Rinse cautiously with water for several minutes; remove contact lenses if present and easy to do; seek medical advice if irritation persists.
  • Ingestion: Rinse mouth; do not induce vomiting; get medical advice.
• Spill response: Absorb with inert material (vermiculite/diatomaceous earth), collect for disposal in a sealable container; prevent release to drains.
• Waste: Dispose as hazardous chemical waste; consider halogenated pesticide-containing organic waste streams per institutional and local regulations.

Always consult the product-specific SDS and CoA for definitive hazard and handling instructions.

Solvent Selection

Applicability

• This product is supplied as a solution. The specific solvent system is Not specified for this item; refer to CoA/Spec Sheet. The guidance below supports method development and dilution planning.

General solvent behavior of cypermethrin (literature)

• Polarity: Nonpolar to moderately polar analyte with high hydrophobicity (log Kow ~6.6–6.9); favors nonpolar organic phases.
• Water miscibility: Practically insoluble in water; avoid aqueous stock solutions.
• Common analytical solvents: n-hexane, iso-octane, toluene, acetone, acetonitrile, ethyl acetate, methyl tert-butyl ether (MTBE). Choice is driven by instrument compatibility and method (GC vs LC).

Selection tips

• GC methods: Hexane or iso-octane are frequent carriers for ECD or MS; toluene may reduce volatility losses and adsorption. Avoid active polar protic solvents in GC vials.
• LC-MS/MS: Acetonitrile or methanol as diluent/strong solvent; include 0.1% formic acid only if validated (ester stability can be affected by basic additives; acidic modifiers are generally safer).
• Stability: Ester hydrolysis is base-catalyzed; avoid alkaline solutions and prolonged exposure to moisture and heat. Protect from light to minimize photolysis.

Brief comparison (literature tendencies)

• Hexane/iso-octane: excellent GC compatibility; low polarity; minimal ion suppression in APCI.
• Acetonitrile: LC-friendly; mixes with water for gradient methods; good solubility.
• Ethyl acetate/toluene: strong solvating power; useful for extraction and intermediate stocks.

Storage & Reconstitution

Item-specific instructions (from Product Data)

• Storage: Store at -20°C.
• Shipping: Ice chest + ice pads via FedEx DG Service.
• Solution details (solvent, concentration): Not specified for this item; refer to CoA/Spec Sheet.

Best practices for solution standards of hydrophobic pesticides

• Light protection: Store in amber vials or secondary light-protective containers to limit photodegradation.
• Headspace management: Keep containers tightly closed; if feasible, backfill with inert gas (nitrogen/argon) after use to minimize oxidation and solvent evaporation.
• Aliquoting: Upon receipt, divide into single-use aliquots to avoid repeated freeze–thaw cycles and adsorption losses on vial walls and septa.
• Temperature equilibration: Allow vials to warm to room temperature in the dark before opening to prevent moisture condensation. Mix thoroughly (gentle vortex) to ensure homogeneity.
• Working solutions: Prepare dilutions in validated solvents (see Solvent Selection). Label with preparation date, solvent, and concentration; store cold (e.g., 4 °C) and use within your method’s verified holding time.
• Container compatibility: Use low-adsorption amber glass vials with PTFE-lined caps; pre-rinse with the intended solvent to condition surfaces.

Always consult the product’s CoA and SDS for definitive storage stability, shelf-life, and handling requirements specific to this lot.

Structure & Identity

Brief overview: Cypermethrin is a Type II pyrethroid insecticide supplied here as an analytical-standard solution for residue analysis and related QC applications.

• Item-specific (from Product Data)
  • Product: Cypermethrin solution (Analytical standard)
  • CAS: 52315-07-8
  • PubChem CID: 2912
  • Storage: Store at -20°C
  • Shipped: Ice chest + ice pads (FedEx DG Service)
• Not specified for this item; refer to CoA/Spec Sheet
  • SMILES
  • InChIKey
  • Exact solution solvent and concentration
  • Appearance
• Literature structural identity (for reference; mixture of stereoisomers)
  • Molecular formula: C22H19Cl2NO3 (literature)
  • Molecular weight: ~416.31 g/mol (literature)
  • Core features: an ester linking a 3-phenoxybenzyl alcohol moiety to a 2,2-dimethyl-3-(2,2-dichloroethenyl)cyclopropane carboxylate; one cyano group on the benzyl carbon; two chlorine atoms on the vinyl group.
  • Stereochemistry: typically supplied as a mixture of cis/trans and R/S isomers at the cyclopropane and benzylic centers (isomer ratios vary by source). Many analytical methods resolve or report isomer groups.
• 2D description (qualitative)
  • Aromatic segment: para-phenoxy-substituted phenyl ring (diphenyl ether motif).
  • Linker: benzylic carbon bearing a –CH2–O– (benzyloxymethyl) connected to the ester carbonyl and a –CN group.
  • Acid fragment: substituted cyclopropanecarboxylate with gem-dimethyl and a 2,2-dichloroethenyl substituent.

Note: All literature identifiers are provided for general context only; consult the item’s CoA for the definitive identity and concentration of this specific solution.

Synthetic Utility

Limited applicability.

• Cypermethrin is a finished multi-isomer pyrethroid ester rather than a versatile synthetic building block. It is not commonly used as a reagent in organic synthesis.
• For synthetic studies (literature), transformations observed include ester hydrolysis to the corresponding acid/alcohol fragments and oxidative/photolytic degradations, primarily in the context of metabolism or environmental fate, not as preparative routes.
• If your objective is method development or degradation pathway elucidation, controlled hydrolysis (acidic vs basic) and photolysis experiments can be designed using this standard—ensure conditions are validated and consistent with your analytical goals.

Target Specificity

Not applicable.

• This product is not a biological probe, antibody, or inhibitor with a defined macromolecular target binding profile as a product claim. While cypermethrin acts on voltage-gated sodium channels in organisms (literature), this section does not apply to the supplied analytical-standard solution.

Frequently Asked Questions

What does analytical standard mean?

analytical standard is a defined analytical purity tier suitable for quantitative analytical methods. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. analytical standard is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Freezer storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 52315-07-8, the molecular formula is C22H19Cl2NO3, and the molecular weight is 416.3 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.