Diaminofluorescein 2T (DAF-2T) - BioReagent, for Microscopy, for Fluorescence analysis, ≥95%(HPLC) , CAS No.208850-35-5

CAS: 208850-35-5 Cat. No.: D1454195 Formula: C₂₀H₁₁N₃O₅ Molecular Weight: 373.32 PubChem CID: 10690728

GRADE & PURITY BioReagent, for Fluorescence analysis, for Microscopy, ≥95%(HPLC)

Synonyms

Diaminofluorescein‑2T | DAF‑2 triazole | 4,5-Diaminofluorescein triazole | 3',6'-dihydroxyspiro[2H-furo[3,4-f]benzotriazole-7,9'-xanthene]-5-one | Spiro[5H-furo[3,4-f]benzotriazole-5,9'-[9H]xanthen]-7(1H)-one, 3',6'-dihydroxy- | 4,5-Diaminofluorescein tri

Storage

Protected from light,Store at -20°C,Desiccated

Shipped In

Ice chest + Ice pads

Application

Fluorescence Analysis

Overview

DAF‑2T, Fluorescent reference material for DAF‑2. Highly fluorescent reference material for DAF‑2 (D131457) . DAF‑2 is membrane impermeable and reacts with Nitric Oxide to generate the highly fluorescent triazole DAF‑2T.

Spectral data: λex 495nm; λem 515nm.

Specifications

Synonyms

Diaminofluorescein‑2T | DAF‑2 triazole | 4,5-Diaminofluorescein triazole | 3',6'-dihydroxyspiro[2H-furo[3,4-f]benzotriazole-7,9'-xanthene]-5-one | Spiro[5H-furo[3,4-f]benzotriazole-5,9'-[9H]xanthen]-7(1H)-one, 3',6'-dihydroxy- | 4,5-Diaminofluorescein tri

Specifications & Purity

BioReagent, for Microscopy, for Fluorescence analysis, ≥95%(HPLC)

Stability And Storage

Store at -20℃ long term (24 months). Store in the dark. Desiccated.

Storage

Protected from light,Store at -20°C,Desiccated

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, for Fluorescence analysis, for Microscopy

Purity

≥95%(HPLC)

Names and Identifiers

• Canonical Smiles: C1=CC2=C(C=C1O)OC3=C(C24C5=CC6=NNN=C6C=C5C(=O)O4)C=CC(=C3)O
• IUPAC Name: 3',6'-dihydroxyspiro[2H-furo[3,4-f]benzotriazole-7,9'-xanthene]-5-one
• InChIKey: AGQDGLGMJJDMKK-UHFFFAOYSA-N
• INCHI: 1S/C20H11N3O5/c24-9-1-3-12-17(5-9)27-18-6-10(25)2-4-13(18)20(12)14-8-16-15(21-23-22-16)7-11(14)19(26)28-20/h1-8,24-25H,(H,21,22,23)
• Isomeric SMILES: C1=CC2=C(C=C1O)OC3=C(C24C5=CC6=NNN=C6C=C5C(=O)O4)C=CC(=C3)O
• PubChem CID: 10690728
• Molecular Weight: 373.32

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organoheterocyclic compounds
    • Class Benzopyrans
      • Subclass 1-benzopyrans
        • Level5 Dibenzopyrans
          • Level6 Xanthenes

• Kingdom: Organic compounds
• Superclass: Organoheterocyclic compounds
• Class: Benzopyrans
• Subclass: 1-benzopyrans
• Intermediate Tree Nodes: Dibenzopyrans
• Direct Parent: Xanthenes
• Alternative Parents: Diarylethers Phthalides Benzofuranones Benzotriazoles 1-hydroxy-2-unsubstituted benzenoids Triazoles Heteroaromatic compounds Lactones Carboxylic acid esters Oxacyclic compounds Azacyclic compounds Organonitrogen compounds Organic oxides Hydrocarbon derivatives
• Molecular Framework: Aromatic heteropolycyclic compounds
• Substituents: Xanthene - Diaryl ether - Benzofuranone - Isobenzofuranone - Phthalide - Benzotriazole - Isocoumaran - 1-hydroxy-2-unsubstituted benzenoid - Phenol - Benzenoid - Azole - Heteroaromatic compound - Triazole - 1,2,3-triazole - Lactone - Carboxylic acid ester - Ether - Carboxylic acid derivative - Oxacycle - Azacycle - Hydrocarbon derivative - Organooxygen compound - Organonitrogen compound - Organic oxide - Organic nitrogen compound - Organic oxygen compound - Aromatic heteropolycyclic compound
• Description: This compound belongs to the class of organic compounds known as xanthenes. These are polycyclic aromatic compounds containing a xanthene moiety, which consists of two benzene rings joined to each other by a pyran ring.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Chemical and Physical Properties

• Solubility: Soluble in DMSO.