10mM in DMSO for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -80°C Ships Dry ice packs + Cold packs Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 1 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Information
Daphnetin, a natural coumarin derivative, is a protein kinase inhibitor, inhibitsEGFR,PKAand PKC withIC50of 7.67 μM, 9.33 μM and 25.01 μM, respectively, also known to exhibit anti-inflammatory and anti-oxidant activities.
In vitro
Daphnetin strongly inhibits not only EGF receptor-catalyzed tyrosine phosphorylation of exogenous substrate but also PKA and PKC activities. Daphnetin inhibits MCF-7 estrogen-responsive human carcinoma cell line at 24-hour exposure with IC50 of 73 μM. Daphnetin reduces the level of cyclin D1 even at 50 μM. Daphnetin protects the cortical neurons against dexamethasone-induced reduction of cell viability in a dose-dependent manner. Daphnetin inhibits the activities of endogenous or recombinant TaPRK in a specific and dose-dependent manner. daphnetin causes a 50% inhibition (IC50) of 3H-hypoxanthine incorporation by Plasmodium falciparum at concentrations between 25 μM and 40 μM. Daphnetin inhibits the mitogenic signalling of ERK1/ERK2.
In vivo
Daphnetin at 140 mg/kg significantly decreases uterine weights by 39.5%. Two and 8 mg/kg administration of Daphnetin could improve the performance of stress mice in Morris water maze tests and forced swimming tests. Daphnetin significantly prolongs survival of P. yoelli-infected mice.
Cell Data
cell lines：
Concentrations：12.5 μM, 25 μM, 50 μM, 100 μM, 200 μM
Incubation Time：24 hours, 48 hours, and 72 hours
Powder Purity:≥99%
| Isomeric SMILES | C1=CC(=C(C2=C1C=CC(=O)O2)O)O |
|---|---|
| RTECS | GN6382600 |
| Molecular Weight | 178.14 |
| Beilstein | 18(5)3,211 |
| Reaxy-Rn | 9372 |
| Reaxys-RN_link_address | https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=9372&ln= |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Solubility | Solubility (25°C) In vitro DMSO: 52 mg/mL (201.37 mM); Ethanol: 52 mg/mL (201.37 mM); Water: Insoluble; |
|---|---|
| Melt Point(°C) | 256°C |
| 1. Yongkui Wang, Jian Chen, Jing Chen, Chao Dong, Xu Yan, Zhongpei Zhu, Pan Lu, Zongmian Song, Hongjian Liu, Songfeng Chen. (2020) Daphnetin ameliorates glucocorticoid-induced osteoporosis via activation of Wnt/GSK-3β/β-catenin signaling. TOXICOLOGY AND APPLIED PHARMACOLOGY, [PMID:33171191] [10.1016/j.taap.2020.115333] |
