GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.Biological Stain?Biological stain grade — dyes characterized for staining cells and tissues. Use in histology and microscopy where staining consistency matters.for Fluorescence analysis?Fluorescence-analysis grade — very low fluorescent impurities for clean spectra. Use in fluorescence assays where background interferes.for Microscopy?Microscopy grade — reagents/stains suited to sample prep and imaging. Use in microscopy where clarity and low background are needed.≥98%
BioReagent, Biological Stain, for Fluorescence analysis, for Microscopy, ≥98% Biological Stain,BioReagent,for Fluorescence analysis,for Microscopy for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Protected from light,Store at -20°C,Desiccated Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Di‑4‑ANEPPDHQ is a dye that rapidly responds to changes in membrane potential. It has no intrinsic fluorescence and emits fluorescence only upon binding to cell membranes. It is sufficiently fast to detect transient (millisecond‑scale) membrane‑potential changes in excitable cells. The magnitude of fluorescence change of Di‑4‑ANEPPDHQ is relatively consistent across various tissues, cells and membrane models, typically exhibiting a 10 % fluorescence shift per 100 mV change.
Procedure
1. Preparation of DMSO stock solution: Prepare 1‑10 mM stock solution with DMSO. Note: Aliquot unused stock solution and store at −20 °C; avoid repeated freeze‑thaw cycles.
2. Preparation of working solution: Dilute the stock solution with an appropriate buffer (e.g., serum‑free medium, PBS) to obtain a working solution of 10‑15 μM. Note: The final working concentration should be optimized according to individual experimental systems. It is recommended to explore the optimal concentration within a 10‑fold range of the suggested concentration.
Precautions
1. Briefly centrifuge the vial to spin down contents to the bottom of the tube before use.
2. Fluorescent dyes are susceptible to photobleaching. Minimize light exposure to slow down photobleaching.
3. For research use only. Not for clinical diagnosis or therapy. Not for food or pharmaceutical applications. Do not store in residential premises.
4. For personal safety and health, wear a lab coat and disposable gloves during handling.
Specifications
Specifications & Purity
BioReagent, Biological Stain, for Fluorescence analysis, for Microscopy, ≥98%
Stability And Storage
Store at -20℃ long term (12 months). Store in the dark. Desiccated.
Storage
Protected from light,Store at -20°C,Desiccated
Shipped In
Ice chest + Ice pads
This product requires cold chain shipping. Ground and other economy services are not available.
Grade
Biological Stain, BioReagent, for Fluorescence analysis, for Microscopy
Purity
≥98%
Product Line
biological_products
Names and Identifiers
Molecular Weight
665.54
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
