Diethyl pyrocarbonate (DEPC) - Moligand™,≥97% , CAS No.1609-47-8

CAS: 1609-47-8 Cat. No.: D137738 Molecular Weight: 162.14 Beilstein Registry Number: 637031 EC Number: 216-542-8
AVAILABLE TO ORDER
GRADE & PURITY Moligand™ ? Moligand™ — Aladdin's line of ligands and bioactive small molecules. Use for receptor, pathway, and binding studies needing defined small-molecule tools. ≥97%
Synonyms
DEP | 3,3',5,5'-tetraethylbenzidine | Diethyl ester of pyrocarbonic acid | diethyl pyrocarbonate | InChI=1/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H | Diethylpyrocarbonate | diethyl-pyrocarbonate | carbonic acid, ethoxycarbonyl ethyl ester | CAS-1609-4
Storage
Store at 2-8°C,Argon charged
Shipped In
Wet ice
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
5g
D137738-5g
9
$9.90
10g
D137738-10g
5
$10.90
25g
D137738-25g
4
$17.90
100g
D137738-100g
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$42.90
500g
D137738-500g
1
$189.90
Enter a quantity for the sizes you want to add.
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Why this grade

Moligand™,≥97% Moligand™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Store at 2-8°C,Argon charged Ships Wet ice Check lot-specific COA for exact specifications.

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Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

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Literature proof

Cited in 45 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Diethyl Pyrocarbonate has been shown to inhibit ryanodine binding to ryanodine/Ca2+ receptor channels in skeletal muscle in a dose- and time-dependent manner and increases the Ca2+ permeability of SR vesicles. This compound could be useful for specific inactivation of nucleases during isolation of undegraded polynucleotides. Diethyl Pyrocarbonate also inhibits PAF Acetylhydrolase (platelet-activating factor acetyl hydrolase), and has been used as a probe for the topography of 5.8S rRNA in yeast ribosomes.
An inhibitor of ryanodine binding at the ryanodine/Ca2+ channel

Specifications

Synonyms
DEP | 3, 3', 5, 5'-tetraethylbenzidine | Diethyl ester of pyrocarbonic acid | diethyl pyrocarbonate | InChI=1/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2, 1-2H | Diethylpyrocarbonate | diethyl-pyrocarbonate | carbonic acid, ethoxycarbonyl ethyl ester | CAS-1609-4
Specifications & Purity
Moligand™, ≥97%
Biochemical and Physiological Mechanisms
Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.
Storage
Store at 2-8°C, Argon charged
Shipped In
Wet ice
This product requires cold chain shipping. Ground and other economy services are not available.
Grade
Moligand™
Purity
≥97%
Names and Identifiers
Pubchem Sid488179737
Pubchem Sid Urlhttps://pubchem.ncbi.nlm.nih.gov/substance/488179737
Canonical SmilesCCOC(=O)OC(=O)OCC
IUPAC Nameethoxycarbonyl ethyl carbonate
InChIKeyFFYPMLJYZAEMQB-UHFFFAOYSA-N
INCHI1S/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H3
Isomeric SMILES CCOC(=O)OC(=O)OCC
WGK Germany 3
RTECS LQ9350000
Molecular Weight 162.14
Beilstein 637031
Reaxy-Rn 637031
Reaxys-RN_link_address https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=637031&ln=

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Taxonomic Classification

Taxonomy Tree

KingdomOrganic compounds
SuperclassOrganic acids and derivatives
ClassOrganic carbonic acids and derivatives
SubclassNot available
Intermediate Tree Nodes Not available
Direct ParentOrganic carbonic acids and derivatives
Alternative Parents Organic oxides  Hydrocarbon derivatives  Carbonyl compounds  
Molecular FrameworkAliphatic acyclic compounds
Substituents Carbonic acid derivative - Organic oxygen compound - Organic oxide - Hydrocarbon derivative - Organooxygen compound - Carbonyl group - Aliphatic acyclic compound
DescriptionThis compound belongs to the class of organic compounds known as organic carbonic acids and derivatives. These are compounds comprising the organic carbonic acid or a derivative thereof.
External Descriptors a small molecule
3D Structure
Interactive Chemical Structure Model





Associated Targets(Human)
ALDH1A1 Tchem Aldehyde dehydrogenase 1A1 (77053 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
SOAT2 Tchem Acyl coenzyme A:cholesterol acyltransferase 2 (288 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Associated Targets(non-human)
Soat1 Acyl coenzyme A:cholesterol acyltransferase 1 (295 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Mechanisms of Action
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

41 results found

Lot NumberCertificate TypeDateItem
F2313816Certificate of AnalysisJun 11, 2026 D137738
F2313659Certificate of AnalysisJun 11, 2026 D137738
E2620369Certificate of AnalysisMay 26, 2026 D137738
E2620365Certificate of AnalysisMay 26, 2026 D137738
H2501352Certificate of AnalysisMay 11, 2026 D137738
H2501351Certificate of AnalysisMay 11, 2026 D137738
H2501345Certificate of AnalysisMay 11, 2026 D137738
H2501344Certificate of AnalysisMay 11, 2026 D137738
H2501343Certificate of AnalysisMay 11, 2026 D137738
G2508069Certificate of AnalysisApr 07, 2026 D137738
A2305338Certificate of AnalysisApr 03, 2026 D137738
F2418367Certificate of AnalysisFeb 05, 2026 D137738
F2313823Certificate of AnalysisFeb 04, 2026 D137738
F2418365Certificate of AnalysisJan 20, 2026 D137738
F2313813Certificate of AnalysisJan 20, 2026 D137738
A2305453Certificate of AnalysisJan 20, 2026 D137738
F2313808Certificate of AnalysisOct 29, 2025 D137738
J2210243Certificate of AnalysisOct 13, 2025 D137738
L2108290Certificate of AnalysisOct 13, 2025 D137738
A2305450Certificate of AnalysisSep 09, 2025 D137738
D2621030Certificate of AnalysisAug 05, 2025 D137738
F2418368Certificate of AnalysisJun 21, 2024 D137738
F2418366Certificate of AnalysisJun 21, 2024 D137738
F2418364Certificate of AnalysisJun 21, 2024 D137738
I2219387Certificate of AnalysisJul 18, 2023 D137738
I2219388Certificate of AnalysisJul 14, 2023 D137738
J2210244Certificate of AnalysisJul 14, 2023 D137738
C2127039Certificate of AnalysisJul 07, 2023 D137738
C2127040Certificate of AnalysisJul 07, 2023 D137738
C2127042Certificate of AnalysisJul 07, 2023 D137738
F2313671Certificate of AnalysisJun 26, 2023 D137738
L2108304Certificate of AnalysisJun 14, 2023 D137738
L2108299Certificate of AnalysisJun 14, 2023 D137738
D2408097Certificate of AnalysisJan 09, 2023 D137738
A2305455Certificate of AnalysisJan 09, 2023 D137738
A2305340Certificate of AnalysisJan 09, 2023 D137738
A2305339Certificate of AnalysisJan 09, 2023 D137738
A2305446Certificate of AnalysisJan 09, 2023 D137738
K2108033Certificate of AnalysisSep 09, 2022 D137738
K2025018Certificate of AnalysisAug 09, 2022 D137738
C2127041Certificate of AnalysisJan 18, 2022 D137738

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Chemical and Physical Properties
SolubilitySoluble in chloroform, ethanol (50% w/w), esters, ketones, and hydrocarbons.Slowly soluble in water with hydrolytic decomposition yielding ethanol and CO2., Soluble in chloroform, ethanol (50% w/w), esters, ketones, and hydrocarbons.Slowly soluble in wate
SensitivityHeat and Moisture sensitive
Refractive Indexn20/D 1.398
Flash Point(°F)156.2 °F
Flash Point(°C)69℃
Boil Point(°C)93-94 °C
Molecular Weight162.140 g/mol
XLogP31.400
Hydrogen Bond Donor Count0
Hydrogen Bond Acceptor Count5
Rotatable Bond Count6
Exact Mass162.053 Da
Monoisotopic Mass162.053 Da
Topological Polar Surface Area61.800 Ų
Heavy Atom Count11
Formal Charge0
Complexity126.000
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
The total count of all stereochemical bonds0
Covalently-Bonded Unit Count1
Documents & Articles
Citations of This Product
References
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20. Liping Yang, Jiacheng Xing, Danhua Yuan, Lin Li, Yunpeng Xu, Zhongmin Liu.  (2021)  Synthesis of high-crystallinity MIL-125 with outstanding xylene isomer separation performance.  CHINESE JOURNAL OF CATALYSIS,      [PMID:] [10.1016/S1872-2067(21)63905-0]
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41. Hong Tao, Shuyun Weng, Luyun Xu, Jianqing Ye, Min Fan, Yong Wang, Yao Lin, Duo Lin, Qingshui Wang, Shangyuan Feng.  (2024)  Target-triggered assembly of plasmon resonance nanostructures for quantitative detection of lncRNA in liver cancer cells via surface enhanced Raman spectroscopy.  BIOSENSORS & BIOELECTRONICS,      [PMID:38905860] [10.1016/j.bios.2024.116488]
42. Jing Xu, Yinbing liu, Xinqi Luo, Yujin Li, Yiqing Xing, Ke-Jing Huang.  (2024)  Visual self-powered platform for ultrasensitive heavy metal detection designed on graphdiyne/graphene heterojunction and DNAzyme-triggered DNA circuit strategy.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2024.150151]
43. Luyun Xu, Yongshi Shen, Zhizhong Lin, Jianqing Ye, Lingna Wang, Xueliang Lin, Shuhang Liu, Duo Lin, Yuanji Xu, Zongyang Yu, Rongcai Zhang, Shangyuan Feng.  (2025)  A catalytic hairpin assembly-assisted magnetic SERS platform with core-satellite structure for ultra-sensitive circRNA detection in early lung cancer screening.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2025.163309]
44. Weidong Chen, Zelin Yang, Yuheng Luo, Jianxun Huang, Lijie Wu, Wenxiong Liu, Yifei Wang, Jiajia Wu, Yining Li, Fang Sun, Pengming Song, Baoce Sun, Qiang Xu.  (2025)  Flowthrough thermal regulation enables ultrafast real-time PCR.  Cell Reports Physical Science,      [PMID:] [10.1016/j.xcrp.2025.103052]
45. Kan Yang, Changan Cheng, Youjia Zhang, Zhaoying Liu, Enze Sheng, Zhen Zhang, Ming Li.  (2026)  Tri-mode fluorescence/colorimetric/strip aptasensor with CRISPR/Cas12a-driven cascade strategy for mycotoxin.  Current Research in Food Science,      [PMID:41568343] [10.1016/j.crfs.2026.101303]
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