DTPD-Q - ≥95% , CAS No.252950-56-4

CAS: 252950-56-4 Cat. No.: D1453828 Formula: C₂₀H₁₈N₂O₂ Molecular Weight: 318.37

GRADE & PURITY ≥95%

Synonyms

2,5-Bis(o-tolylamino)cyclohexa-2,5-diene-1,4-dione | 2,5-Bis[(2-methylphenyl)amino]-2,5-cyclohexadiene-1,4-dione

Storage

Store at -20°C,Argon charged

Shipped In

Ice chest + Ice pads

Overview

DTPD-Q is an antioxidant and an oxidative derivative of methylbenzenesulfonyl-p-phenylenediamine DTPD. DTPD-Q is a molecule that binds to AKT1 and ESR1, with high binding affinity to core targets associated with male infertility and its subtypes. DTPD-Q can be used in studies related to male infertility

Specifications

Synonyms

2,5-Bis(o-tolylamino)cyclohexa-2,5-diene-1,4-dione | 2,5-Bis[(2-methylphenyl)amino]-2,5-cyclohexadiene-1,4-dione

Specifications & Purity

≥95%

Storage

Store at -20°C,Argon charged

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Purity

≥95%

Names and Identifiers

• Molecular Weight: 318.37

Chemical and Physical Properties

• Sensitivity: Air and heat sensitive
• Melt Point(°C): 254 °C

Application Protocols

Item-specific tested applications and conditions: Not specified for this item; refer to CoA/Spec Sheet.

General recommendations (not item-specific):

• If used in biochemical assays, prepare fresh working solutions immediately before use. Validate concentration by UV/Vis or HPLC where possible.
• For cell-based work, filter-sterilize buffer dilutions (0.22 μm) when appropriate and limit DMSO to assay-tolerable levels.
• Record lot number, preparation date, and storage conditions in lab notebooks to ensure traceability and reproducibility.

No protocol parameters (e.g., WB/IHC dilutions, incubation times) can be offered without product-specific validation.

Biological Roles

Item-specific biological function: Not specified for this item; refer to CoA/Spec Sheet.

General note:

• The product is categorized under life science in the catalog path, but no structural or functional annotation is provided. Without confirmed identity, no credible statements can be made regarding biochemical pathways, targets, or cellular roles.
• If the compound is intended as a biochemical probe or modulator, users should consult primary references linked by CAS or internal documentation and independently validate activity under their assay conditions.

Reminder: For research use only. Not for human or veterinary use.

Buffer Applications

Item-specific buffer compatibility: Not specified for this item; refer to CoA/Spec Sheet.

General guidance (not item-specific):

• If preparing assay stocks of sensitive small molecules, dissolve initially in anhydrous DMSO (or other dry, oxygen-free solvent) and dilute into buffer immediately prior to use. Common biological buffers include PBS (pH ~7.4), HEPES (pH 6.8–8.2), and Tris (pH 7–9). Ensure final cosolvent levels are compatible with cells/enzymes.
• Avoid repeated freeze–thaw of working solutions. Prepare single-use aliquots to maintain integrity.
• For potentially hydrolysis-prone compounds, avoid high-pH buffers and minimize exposure time in aqueous media; run stability-indicating analytics (HPLC/LC–MS) to confirm recovery over the assay timeframe.

Because the compound’s ionization behavior is unknown, no pH-dependent solubility or buffering recommendations can be made beyond these general practices.

Green Alternatives

Item-specific alternatives: Not applicable; structural class and use-case are not specified for this item.

General sustainability guidance (not item-specific):

• Solvent selection has the largest environmental impact lever. Where feasible, prefer lower-toxicity, renewable, or higher-boiling, recyclable options: 2-MeTHF over THF; CPME or EtOAc over chlorinated solvents; propylene carbonate or MeOH over DMF/NMP, when performance allows.
• Minimize solvent volumes through concentrated reaction conditions, continuous processing, or high-solids formulations.
• For air-sensitive materials, solvent drying can be achieved using molecular sieves rather than metal hydrides to reduce hazards and waste.
• Packaging: request right-sized pack sizes to reduce opened-container shelf time and waste risk for sensitive reagents.

Trade-offs (general):

• Greener solvents may alter kinetics/selectivity or solubility. Validate on small scale and benchmark yields, E-factor, and energy consumption before full deployment.

Pharmaceutical Uses

Item-specific pharmacopeial status, excipient role, or formulation use: Not specified for this item; refer to CoA/Spec Sheet.

General statement (compliance):

• Supplied for research use only. Not intended for use in humans or as an active pharmaceutical ingredient (API) or excipient in commercial drug products without additional qualification.
• If formulation studies are pursued in preclinical research, ensure material controls cover identity, purity, stability, and residual solvents to research standards, and conduct appropriate risk assessments.

Physical Properties

Item-specific properties (from Product Data):

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Melting point / boiling point: Not specified for this item; refer to CoA/Spec Sheet.
• Density, refractive index, viscosity: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility profile (water/organic): Not specified for this item; refer to CoA/Spec Sheet.
• LogP/logD, pKa: Not specified for this item; refer to CoA/Spec Sheet.

General guidance (literature/typical practice; not item-specific):

• For solids stored at −20 °C under inert gas, limited volatility is common; for liquids under argon, air/moisture sensitivity rather than volatility may be the controlling factor.
• If solubility screening is needed, a tiered approach is typical: begin with dry, oxygen-free solvents (e.g., anhydrous DMSO, DMF, MeCN, THF, toluene, or ethanol), then expand as needed. Always validate by small-scale tests.
• If the compound is moisture-sensitive, Karl Fischer or coulometric titration of solvent water content can be valuable before dissolution (general best practice).

Note: Definitive numerical values must be taken from the product’s CoA/Spec Sheet or SDS. No property values are specified for this item.

Quality & Grades

Item-specific quality attributes (from Product Data):

• Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.

What the provided logistics imply:

• Cold-chain shipment (ice chest + ice pads) and argon-charged storage typically indicate a stability-limited or air/moisture-sensitive material. This often correlates with tighter controls on water and oxygen exposure during packaging, though no item-specific limits are provided here.

General notes on grades (informational; not item-specific):

• Research grade: intended for laboratory R&D; may not meet pharmacopeial monographs.
• Purity reporting: can be by HPLC/GC/area% or qNMR; verify the analytical basis, chromatographic conditions, and reporting threshold on the CoA.
• Residual solvents, water (KF), metals (ICP), and stabilizers/inhibitors: Not specified for this item; refer to CoA/Spec Sheet. Do not assume absence.

Recommendations:

• Review the batch-specific CoA for assay method, impurity profile, water content, and any stabilizer. If the material is oxygen/moisture sensitive, request packaging under inert atmosphere and minimize transfers.

Reaction & Applications

Manufacturer-supplied application text: Not provided for this item.

Item-specific reactivity information: Not specified for this item; refer to CoA/Spec Sheet.

General guidance (contextual; not item-specific):

• The argon-charged storage suggests potential reactivity toward oxygen and/or water. If used in synthesis, maintain inert conditions (dry glassware, Schlenk or glovebox techniques) and add to reactions via gas-tight syringes or cannula.
• If the material functions as a building block or ligand, ensure stoichiometry accounts for potential adventitious hydrolysis or oxidation; titration or assay prior to use can improve reproducibility.
• For analytical or life-science assay applications, prepare concentrated stock in an anhydrous, oxygen-free solvent (commonly DMSO) and dilute freshly into working buffers immediately prior to use to limit degradation.

Note: Without structural information, no specific named reactions or mechanistic roles can be responsibly recommended. Consult primary literature using the CAS RN and the Aladdin CoA/SDS for definitive application scope.

Reaction Conditions

Item-specific optimal conditions: Not specified for this item; refer to CoA/Spec Sheet.

General practices for handling sensitive reagents (not item-specific):

• Atmosphere: Argon or nitrogen, with oven-dried glassware. Where appropriate, glovebox handling can improve reproducibility.
• Solvents: Use anhydrous, degassed solvents. Verify water content by Karl Fischer if necessary.
• Temperature: Store and, if necessary, charge reactions at low temperature to limit decomposition; then warm as reactivity permits.
• Additions: Use gas-tight syringes or cannula transfers to avoid air ingress. Add sensitive reagents last.
• Monitoring: Employ rapid analytical checks (TLC, GC/HPLC, in situ IR) to track consumption and side-product formation.

Expected yields/timelines cannot be provided without structure-specific literature.

Safety & Handling

Item-specific hazard data (from Product Data):

• GHS Classification: Not specified for this item; refer to SDS.
• Signal Word / Pictograms / H-Statements: Not specified for this item; refer to SDS.

Confirmed handling/storage from Product Data:

• Storage: Store at −20 °C, argon charged.
• Shipping: Ice chest with ice pads (cold chain).

Practical handling guidance (general; defer to SDS for authority):

• Assume sensitivity to oxygen and/or moisture given the argon charge. Handle under inert atmosphere (argon or nitrogen) using dry glassware. Minimize headspace exchange; recap promptly.
• Personal protective equipment: lab coat, safety glasses, and appropriate chemically resistant gloves. Work in a fume hood to control vapors/particulates and prevent atmospheric moisture ingress.
• Incompatibilities to consider (general): oxidizing agents, aqueous bases/acids, and humid air if hydrolysis is possible. Do not heat or sonicate in open air.
• First-aid (generic): Inhalation—move to fresh air; Skin—wash with soap/water; Eyes—rinse cautiously with water for several minutes; Ingestion—rinse mouth. Seek medical attention and present SDS.
• Spill/cleanup: Quench reactivity only if compatible is known. Otherwise, contain, absorb with inert material, and collect for disposal as hazardous waste. Avoid water if moisture-sensitive.

Always consult the product-specific SDS for definitive hazards, exposure limits, stability, and disposal guidance.

Solvent Selection

Item-specific solvent data: Not specified for this item; refer to CoA/Spec Sheet.

General selection strategy for air/moisture-sensitive materials (inferred from argon-charged storage; not item-specific):

• Prefer rigorously dried, oxygen-free solvents. Typical first-line choices: anhydrous DMSO or DMF for polar substrates; anhydrous MeCN, THF, 2-MeTHF, toluene, or EtOAc depending on polarity.
• Degas solvents (argon sparge or freeze–pump–thaw) if the compound is oxygen-labile; use molecular sieves (3Å/4Å) where compatible.
• Match solvent to downstream use: analytical (LC–MS grade for trace analysis), reactions (water-free, inhibitor-free), or biotesting (cell-compatible vehicles; DMSO typically ≤0.5–1% v/v in assay).

Brief comparison (general):

• DMSO vs DMF: DMSO offers broad solvency and assay compatibility; DMF is lower viscosity and often preferred synthetically but less biocompatible.
• THF/2-MeTHF: higher volatility and easy removal; ensure peroxide control for ethers (monitor and use inhibitors if appropriate).

Always verify actual solubility and stability of this specific product by small-scale trials and consult the CoA for any solvent restrictions.

Storage & Reconstitution

Item-specific instructions (from Product Data):

• Storage: Store at −20 °C, argon charged.
• Shipping: Ice chest + ice pads (maintain cold chain during transit).

General best practices for this class of materials (not item-specific):

• Upon receipt: Allow container to equilibrate to room temperature before opening to prevent moisture condensation. Open under inert gas if possible. Reseal promptly; backfill with argon.
• Aliquoting: If repeated access is expected, subdivide into small, dry, inert-atmosphere aliquots to avoid freeze–thaw and headspace oxygen ingress.
• Reconstitution: Because the specific solvent is not provided, evaluate solubility on a small scale using anhydrous, oxygen-free solvents (e.g., DMSO, DMF, MeCN, THF, ethanol, or toluene) as appropriate for the intended use. Filter through a dry PTFE syringe filter if particulates are present.
• Stability: Track solution stability by analytical methods (HPLC/LC–MS) and use freshly prepared solutions when possible. Store solutions at low temperature under inert gas if compatible.

Specifications not provided for this item (consult CoA/SDS): solubility limits, pH stability, light sensitivity, water content, and presence/absence of stabilizers or inhibitors.

Structure & Identity

Item-specific facts (from Product Data):

• Product Name: DTPD-Q (SKU D1453828)
• CAS: 252950-56-4
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• InChIKey: Not specified for this item; refer to CoA/Spec Sheet.

General notes (context; not item-specific):

• Without a structure, functional group content, ring systems, and stereochemistry cannot be confirmed. The CAS RN uniquely identifies a single chemical substance, but structural descriptors (SMILES/InChI) are required for computational or registry cross-referencing.
• The storage guidance (−20 °C, argon charged) often indicates sensitivity to oxygen and/or moisture; however, exact functional groups or instability motifs (e.g., acid chlorides, boronic species, air-sensitive organometallics, thiols, aldehydes, radical initiators) are not confirmed for this item.

Synthetic Utility

Item-specific synthetic role and functional groups: Not specified for this item; refer to CoA/Spec Sheet.

General commentary (not item-specific):

• For air/moisture-sensitive reagents (as suggested by argon-charged storage), synthetic value often lies in delivering reactive motifs that would otherwise degrade (e.g., organometallics, activated electrophiles, redox-labile fragments). Such reagents require inert techniques and dry solvents to preserve activity.
• If the material is a complex small-molecule intermediate, consider protecting-group strategy, chemoselectivity under anhydrous conditions, and rapid work-up protocols that minimize water contact.

No specific named transformations can be assigned without the compound’s structure and reactivity profile.

Target Specificity

Item-specific target/epitope data: Not provided. No antigen, clone, isotype, or species cross-reactivity information is supplied for this item.

Interpretation:

• This section is typically applicable to antibodies, proteins, or affinity reagents. In the absence of such data, target specificity is not applicable to this product as listed. Please refer to the CoA or product page if future lots include bioaffinity metadata.