GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)1.0 mg/mL
Binds to chondroitin sulfate on the cell surface to provide virion attachment to target cell.
Host species
Mouse
Specificity
E8L
Immunogen
Recombinant protein corresponding to MPXV E8L
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
Light Chain Type
kappa
SDS-PAGE
150.0 KDa
Purification Method
Protein A purified
Matched Pairs
[Ab156143] E8L Mouse mAb, Capture Antibody
Concentration
1.0 mg/mL
Storage and Shipping
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Images
Sandwich ELISA - E8L standard curve Sandwich ELISA detection of Recombinant MPXV E8L Protein (rp155937) using E8L Mouse mAb (Ab156143) as capture antibody at concentration of 1.0 µg/mL. Biotinylated E8L Mouse mAb (Ab179942) as detection antibody at concentration of 1.0 µg/mL. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148). Detection range: 0.008 ng/mL - 125.0 ng/mL
Indirect ELISA - E8L standard curve Immobilized Recombinant MPXV E8L Protein (rp155937) at 1.0 µg/mL overnight at 4℃. Coated protein probed with E8L Mouse mAb (Ab179942). Goat Anti-Mouse IgG H&L (HRP) (Ab156255) detected bound primary antibody. EC50: 5.267 ng/mL
Application
Application
Dilution info
ELISA
0.5-2000 ng/mL
Detection Antibody
1.0 μg/mL
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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