EMSA/Gel-Shift Loading Buffer (4×, Blue) - BioReagent, Suitable for electrophoresis, for Protein analysis, Bromophenol Blue Contained

Cat. No.: E1547551

GRADE & PURITY BioReagent, Suitable for electrophoresis, for Protein analysis, Bromophenol Blue Contained

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Application

1151,1152

Overview

This product is suitable for EMSA (Gel-Shift Assay) to study the binding between target proteins and specific DNA sequences, so as to evaluate the activation level of transcription factors in cells. It is designed for sample loading after binding reactions between nuclear proteins or purified transcription factors and specific double-stranded oligonucleotides.

Notes & Alternative Option: 

This product contains bromophenol blue as an electrophoresis tracking dye. In certain EMSA systems, bromophenol blue may interfere with the binding reaction between the target transcription factor and double-stranded oligonucleotides (e.g., altering the migration of binding complexes or affecting signal detection).

If your assay is dye-sensitive, we recommend the bromophenol-blue-free alternative: EMSA/Gel-Shift Loading Buffer (4×, colorless) (Aladdin Cat. No.: E1547550).

Instructions for Use:

Mix at a ratio of 1 μL of loading buffer (4×) per 3 μL of sample. After mixing thoroughly, load immediately.

Precautions:

1.All other reagents required for EMSA must be provided by the user.

2.This product is for research use by professionals only. It must not be used for clinical diagnosis or treatment, for food or drugs, or stored in ordinary residential buildings.

3.For your safety and health, please wear appropriate personal protective equipment and clothing during operation.

Specifications

Specifications & Purity

BioReagent, Suitable for electrophoresis, for Protein analysis, Bromophenol Blue Contained

Stability And Storage

Store at -20℃ long term (12 months). Avoid repeated freeze-thaw cycles.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, for Protein analysis, Suitable for electrophoresis

Product Line

Biological Products