Enterobacteria Enrichment Broth (USP) - BioReagent

Cat. No.: E1528473

GRADE & PURITY BioReagent

Storage

Room temperature

Shipped In

Normal

Overview

For enrichment cultivation of intestinal bacteria. Preparation method: Weigh 45.0 g of this product in 1 L of distilled or deionized water (the volume may be scaled up or down proportionally), boil in a water bath for 30 minutes, cool to room temperature, and set aside for use.

Specifications

Specifications & Purity

BioReagent

Storage

Room temperature

Shipped In

Normal

Grade

BioReagent

Product Line

Biological Products

Application Protocols

No manufacturer-validated protocols are provided in the Product Data. The following is general literature guidance; adapt to your SOP and compendial method.

General enrichment workflow (literature, general)

1. Reconstitution: Weigh the labeled grams of dehydrated medium per liter of purified water. Dissolve with stirring and gentle heat.
2. Sterilization: Autoclave at 121 °C for 15 min (unless otherwise specified). Cool to room temperature. Inspect for clarity/expected appearance.
3. Sample preparation: For solids, aseptically transfer the validated mass/portion into a sterile container with the appropriate volume of broth. For liquids, inoculate the validated volume directly. Include neutralizers if required for preservatives/disinfectants.
4. Incubation: 35–37 °C for 18–24 h (typical). Loosen caps for aeration. Do not shake unless specified.
5. Subculture/Detection: Streak a loopful onto a selective/differential agar (e.g., MacConkey). Alternatively, proceed to biochemical panels or rapid detection methods per method.
6. Controls: Include positive control (e.g., E. coli ATCC strain) and negative controls (e.g., S. aureus and an uninoculated broth control) for each batch.

Notes

• Record lot numbers, preparation date, sterilization parameters, and pH (if measured).
• Discard prepared media showing contamination, precipitation, or unexpected color change.

Biological Roles

This product does not have intrinsic biological roles as a single molecule; it is a formulated medium designed to support and selectively enrich members of the Enterobacteriaceae family.

• Functional intent (literature, general)

  • Nutritional support: Provides peptides, amino acids, vitamins, and carbohydrates to promote rapid resuscitation and growth of sublethally injured Enterobacteriaceae.
  • Selectivity: Incorporates inhibitory components to suppress competing Gram-positive flora and some non-target Gram-negatives, thereby increasing the relative abundance of Enterobacteriaceae during enrichment.
  • Buffering: Maintains pH within a range favorable to target organisms despite acid production from carbohydrate fermentation.

• Typical target organisms (examples; not exhaustive)

  • Escherichia, Enterobacter, Klebsiella, Citrobacter, Serratia, Salmonella, Shigella (literature, general). Final confirmation requires downstream biochemical or molecular identification; enrichment alone is not definitive.

• Role in workflows

  • Used as a pre-enrichment step prior to plating on selective/differential agars or applying rapid ID systems. Enhances detection sensitivity in low bioburden samples or in matrices with inhibitory substances.

• Research use limitation (from Product Data)

  • For research use only. Not intended for diagnostic procedures in humans or animals.

Buffer Applications

This product is a complex culture medium rather than a defined buffering reagent. While it contains buffering salts to stabilize pH during microbial growth, it is not typically used as a general-purpose buffer outside microbiological enrichment.

• Practical notes (literature, general)
  • Prepared medium provides intrinsic pH control suitable for Enterobacteriaceae enrichment; the exact buffer system and target pH are formulation-dependent. Not specified for this item; refer to CoA/Spec Sheet.
  • If pH adjustment is required by a validated method, use sterile 1 N acids/bases and verify pH at room temperature on the reconstituted broth before sterilization.
  • For electrophoresis, protein chemistry, or enzyme assays, use dedicated buffering systems (e.g., phosphate, Tris, HEPES) rather than culture media.

Green Alternatives

While culture media are generally aqueous and align with green chemistry principles more readily than many organic solvents, sustainability considerations still apply.

• Current product profile (general)

  • Aqueous use-phase, room-temperature storage, and normal shipping reduce energy intensity relative to refrigerated, solvent-based reagents.
  • Dehydrated format minimizes transport mass/volume and packaging waste compared with ready-to-use liquid media.

• Potential greener choices or practices (literature, general)

  • Media optimization: Use exactly the required volume and concentration; prepare small batches to minimize expired waste.
  • Sterilization efficiency: Autoclave full loads, use validated cycles, and avoid overprocessing to reduce energy consumption and component degradation.
  • Waste minimization: Autoclave and dispose of spent media following local regulations; consider biodegradable bags for biohazard waste where permitted.

Comparison (general)

• Enterobacteria Enrichment Broth vs. ready-to-use bottled broth
  • Resource use: Dehydrated powder has lower shipping weight and plastic usage; bottled broth offers convenience but higher packaging footprint.
  • Shelf life: Powders typically have longer unopened shelf life; liquids require cold-chain in some cases and have higher transport emissions.

Trade-offs

• Selective agents (e.g., bile salts, dyes) can increase aquatic toxicity if released untreated. Ensure proper inactivation and disposal.

Pharmaceutical Uses

No therapeutic or clinical use is implied. In the pharmaceutical context, this medium is used in quality control laboratories.

• Role in pharma QC (literature, general)

  • Employed for selective enrichment of Enterobacteriaceae in compendial microbiological tests aligned with USP chapters (e.g., microbial enumeration, tests for specified microorganisms) and related harmonized methods.
  • Common applications include testing of raw materials, excipients, non-sterile finished products, water systems, and environmental monitoring samples where enrichment increases detection sensitivity.

• Method validation considerations

  • Perform growth promotion, inhibitory, and indicative properties testing with compendial strains upon receipt of each lot and after significant storage intervals.
  • Document equivalence if substituting lots/suppliers within a validated method.

• Regulatory note

  • “USP” in the product name denotes suitability for USP-style methods but is not, by itself, a certification of cGMP manufacture or compendial compliance of individual lots. Always verify against your site’s approved specifications and CoA.

• Excipient/formulation status

  • Not used as a pharmaceutical excipient; intended exclusively as a laboratory culture medium for research/QC use.

Physical Properties

Item-specific properties

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Storage conditions (from Product Data): Room temperature. Protect from moisture.
• Shipping: Normal.

As a dehydrated medium, most conventional pure-compound physical constants (mp/bp, density, refractive index, logP, pKa) are not applicable. Where relevant in use, properties pertain to the prepared broth, not the powder.

Prepared broth properties (literature, general — not item specifications)

• Solvent: Purified/deionized water (Type I/II per lab practice).
• pH: Typically controlled by intrinsic buffering of the formulation; exact target pH is method- and brand-specific. Not specified for this item; refer to CoA/Spec Sheet.
• Osmolality/ionic strength: Determined by the formulation (peptones, salts, carbohydrates); not a controlled analytical reagent parameter for routine use.

Practical handling properties (general)

• Hygroscopicity: Dehydrated media can be moisture-sensitive; keep tightly closed and desiccated to prevent clumping and loss of performance.
• Solubility: Designed to be readily soluble/dispersible in water at the labeled grams-per-liter; gentle heating and agitation aid dissolution (literature, general). Exact reconstitution mass: Not specified for this item; refer to product label/CoA.

Quality & Grades

• Item-specific grade

  • BioReagent: Indicates suitability for laboratory research use, manufactured to support reproducibility in life-science workflows. This is not a clinical/diagnostic device and is not certified for therapeutic use. For research use only (per Product Data).

• “USP” designation context (general)

  • The name Enterobacteria Enrichment Broth (USP) aligns the product with media commonly used in procedures described in United States Pharmacopeia microbiology chapters (e.g., microbial limits, enrichment steps). It does not by itself confer USP certification of the lot; users should verify compliance within their validated methods.

• Item-specific specifications

  • Exact composition, pH, fill mass per liter, and performance criteria: Not specified for this item; refer to CoA/Spec Sheet.

• Performance considerations (literature, general)

  • Growth promotion and inhibitory performance should be verified with appropriate controls (e.g., recovery of representative Enterobacteriaceae and inhibition of Gram-positive organisms) during method qualification or upon receipt of new lots.
  • Low background contamination and consistent nutrient/ inhibitor balance are key to enrichment selectivity. Store dry and reconstitute with high-purity water to maintain performance.

• Stabilizers/indicators

  • If the formulation contains dyes or selective inhibitors, light and moisture protection improve shelf life. Specific stabilizers: Not specified for this item; refer to CoA/Spec Sheet.

Reaction & Applications

As a microbiological medium, this product is not used as a chemical reagent in synthetic reactions. Its applications are biological/culture-based.

Applications (literature, general)

• Selective enrichment: Pre-enrichment of Enterobacteriaceae from non-sterile or heavily contaminated samples (pharmaceutical raw materials, cosmetics, food, environmental swabs) prior to isolation and enumeration on selective/differential agars (e.g., MacConkey, VRBG, EMB).
• Compendial use: Employed in workflows modeled on USP microbiology chapters (e.g., microbial enumeration tests, specified microorganism testing) where enrichment of Enterobacteriaceae is required.
• Method integration: Following incubation (commonly 18–24 h at 35–37 °C), subculture onto selective plates or proceed to confirmatory biochemical/rapid methods per SOP.

Practical tips (general)

• Inoculum handling: For solid samples, use validated sample-to-broth ratios to ensure adequate neutralization of inhibitory matrix components. For liquids, use volumes specified by the method to achieve defined enrichment factors.
• Aeration: Incubate with loose caps to allow gas exchange; shaking is usually not required unless specified by method.
• Neutralizers: If disinfectants/preservatives are present, include validated neutralizers or pre-rinse steps to prevent false negatives.
• Controls: Include positive controls with a representative Enterobacteriaceae strain and negative controls to verify selectivity and sterility.

Item-specific application notes from manufacturer: Not specified for this item.

Reaction Conditions

Chemical reaction conditions do not apply. However, preparation and incubation conditions are critical to performance in microbiological workflows.

Preparation (literature, general)

• Reconstitution: Weigh the labeled grams-per-liter into purified water. Mix with moderate heat and stirring until fully dissolved. Do not overheat carbohydrate-rich media to avoid browning.
• pH: Verify and adjust only if directed by your validated method. Exact targets are formulation-specific; Not specified for this item.
• Sterilization: Autoclave at 121 °C for 15 minutes (typical) unless your method specifies otherwise. Allow to cool to 45–50 °C before dispensing if required.
• Storage of prepared broth: Use promptly. If holding, store at 2–8 °C protected from light and use within the timeframe defined by your SOP. Do not use if contamination, precipitation, or color change is observed.

Incubation (literature, general)

• Temperature/time: Commonly 35–37 °C for 18–24 h for enrichment; adjust per compendial/validated method.
• Atmosphere: Aerobic; vented caps to allow gas exchange.
• Subculture: After enrichment, streak to selective/differential agar or proceed with confirmatory identification tests.

Quality controls (general)

• Positive control: A representative Enterobacteriaceae strain (e.g., E. coli) for growth promotion.
• Negative control: A Gram-positive organism (e.g., S. aureus) to verify selectivity, plus uninoculated sterility control.

Safety & Handling

Item-specific hazard information

• Signal word, H-statements, GHS classification, pictograms: Not specified for this item; refer to the SDS.

General safety guidance for dehydrated culture media (literature, general)

• Likely hazards: Nuisance dust; may cause mechanical irritation to eyes/respiratory tract. Some selective components (e.g., bile salts, dyes) can be irritating. Avoid inhalation and contact with skin/eyes. Handle powders in a fume hood or with local exhaust when weighing.
• PPE: Laboratory coat, safety glasses, and appropriate gloves. Use a dust mask/respirator (e.g., P2/N95) if dust generation cannot be avoided.
• First aid overview: If inhaled, move to fresh air. If on skin, wash with soap and water. If in eyes, rinse cautiously with water for several minutes and remove contact lenses if present and easy to do. If ingested, rinse mouth. Seek medical attention if symptoms persist. Defer to SDS for authoritative measures.
• Incompatibilities: Avoid strong oxidizers and acids/bases that could decompose organic components; keep dry to prevent microbial contamination and premature degradation.
• Sterility: Supplied non-sterile; sterilize prepared broth by autoclaving as required by your test method.
• Biohazard note: After inoculation, treat cultures and spent media as biohazardous waste. Autoclave before disposal per institutional biosafety procedures.

Always consult the product’s SDS and your institution’s chemical hygiene plan.

Solvent Selection

This product is a dehydrated culture medium intended to be dissolved in water; organic solvent selection is not applicable.

• Practical guidance (literature, general)

  • Primary solvent: Purified, deionized water (Type I/II per ASTM/CAP/EP) for reconstitution at the labeled grams-per-liter. Use freshly prepared water free of residual disinfectants (e.g., chlorine) that can inhibit growth.
  • Sterilization: Autoclave the reconstituted broth as directed by the test method; avoid overheating if the formula includes carbohydrate-rich components to minimize Maillard browning.
  • Optional supplements: Some protocols add selective supplements or antibiotics; use only if specified by your validated method.

• When to choose this medium vs. alternatives (general)

  • Choose Enterobacteria Enrichment Broth (USP) for selective pre-enrichment of Enterobacteriaceae from non-sterile samples in compendial workflows.
  • Alternatives: Buffered peptone water (pre-enrichment with minimal selectivity), lactose broth, or selective broths like selenite or tetrathionate (different selectivity spectrum). Selection depends on matrix and target organism profile.

Storage & Reconstitution

Item-specific storage/shipping

• Storage conditions (from Product Data): Room temperature. Keep container tightly closed and protected from moisture.
• Shipped in: Normal (ambient).

Shelf life and stability

• Unopened dehydrated media are typically stable at room temperature when kept dry and away from light. Lot-specific expiry and recommended storage humidity: Not specified for this item; refer to label/CoA.

Reconstitution and sterilization (literature, general)

• Water quality: Use purified/deionized water (Type I/II). Avoid water containing residual sanitizers.
• Reconstitution: Add the labeled grams of powder per liter of water. Stir while gently heating until fully dissolved.
• pH check: If required by your method, verify pH at room temperature before sterilization.
• Sterilization: Autoclave at 121 °C for 15 minutes unless otherwise specified.
• Post-autoclave: Allow to cool; use promptly or store at 2–8 °C (protected from light) for a limited period defined by your SOP. Do not freeze prepared broth unless validated.

Handling

• Aseptically dispense to sterile containers. Minimize repeated warming/cooling cycles.
• Discard any prepared medium that shows turbidity (without inoculation), precipitation, or color change.

Research use note

• For research use only (from Product Data). Not for diagnostic or therapeutic use.

Structure & Identity

This product is a complex, dehydrated culture medium (mixture) formulated for the selective enrichment of Enterobacteriaceae as described in compendial methods.

• Item-specific identifiers

  • SKU: E1528473 (Aladdin Scientific)
  • Product name: Enterobacteria Enrichment Broth (USP)
  • Grade: BioReagent
  • CAS, InChIKey, SMILES, molecular formula, molecular weight: Not applicable to a complex mixture; Not specified for this item; refer to CoA/Spec Sheet.

• Nature of material (general)

  • Type: Dehydrated microbiological growth medium (broth base), USP-named method medium for enrichment of Enterobacteriaceae from non-sterile samples.
  • Composition: Complex, typically includes peptones/tryptones, carbohydrate(s), selective inhibitors (e.g., bile salts) and buffering salts (literature, general). Exact formulation and lot-specific details: Not specified for this item; refer to CoA/Spec Sheet.

• Structural features

  • As a mixture of biologically derived peptones, salts, and inhibitors, this product does not have a single defined 2D molecular structure. Functional classes present commonly include peptides/amino acids (nutrients), inorganic phosphate/salts (buffer), and selective agents that suppress Gram-positive flora (literature, general).

• Regulatory naming note

  • “USP” in the name indicates suitability for procedures aligned with United States Pharmacopeia microbiology chapters; it does not denote a single chemical entity.

Synthetic Utility

Not applicable. This product is a microbiological enrichment medium and is not used as a synthetic building block, catalyst, or solvent.

• If your workflow requires chemical synthesis steps (e.g., preparation of selective supplements or neutralizers), consult reagent-specific documentation. Use the medium strictly for its intended role in microbial enrichment.

Target Specificity

This section (antigen/epitope/clone specificity) is not applicable. The product is a culture medium and does not possess molecular target specificity in the sense of antibodies or inhibitors.

• Context note (general)
  • The medium exhibits biological selectivity at the community level by favoring growth of Enterobacteriaceae while suppressing certain competing organisms. Organism examples are provided under Biological Roles and Reaction & Applications. Item-specific selectivity metrics are not specified; refer to CoA/Spec Sheet and perform growth-promotion/inhibition verification as required.