B5 Fixative Recipe

Caution: Mercuric chloride is a skin and eye irritant and Formaldehyde is a carcinogen. Use protective equipment and prepare under hood in a well ventilated area. Reference proper procedures and disposal.


Recipe

1.Stock solution (stable for approximately 1 year). Mix well and do not use any metal utensils or foil lined lids.

    12.0g Mercuric chloride

    2.5g Sodium acetate

    200ml Distilled water 200 ml

2.Working solution (prepare right before use)

    20ml of stock solution

    2ml of formaldehyde

Categories: Protocols

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