Saturated ammonium sulfate can precipitate globular Pr. The content of globular Pr in normal cerebrospinal fluid is very low. Therefore, the positive result, if the ball Pr increased, then the Ross-jones test tube is positive, after removing the ball Pt and then by acetic acid boiling method ALB (white Pr).
Operation method
Cerebrospinal fluid total protein assay
Principle
Saturated ammonium sulfate can precipitate globular Pr. The content of globular Pr in normal cerebrospinal fluid is very low. Therefore, the positive result, if the ball Pr increased, then the Ross-jones test tube is positive, after removing the ball Pt and then by acetic acid boiling method ALB (white Pr).
Materials and Instruments
Cerebrospinal fluid Move I. Experimental reagents: Caveat 1. If there are too many RBCs, centrifuge the sediment and draw the supernatant for testing. 2. The tubes and tubes used in the test must be very clean, otherwise false positives may occur. 3. Phenol or (NH4Phenol or (NH 4)2SO4can cause false positives if impure, as can room temperatures below 10°C and low phenol saturation. For more product details, please visit Aladdin Scientific website.
Saturated ammonium sulfate solution Acetic acid
l, saturated ammonium sulfate solution, ammonium sulfate 85g plus distilled water to 100ml that is obtained.
2, 0.8mmol / L acetic acid.
Second, the experimental operation:
l. In a small test tube containing 0.5 ml of saturated ( NH4 ) 2SO4 with a dropper will be 0.5 ml of cerebrospinal fluid along the tube to avoid adding Xu Xu, 3 minutes in the two liquid interfaces to form a white turbid ring, because the Ross-jones (+)
2. Will be in the tube of the two liquids vibration mixing, 3 minutes to the appearance of a white cloudy or precipitate, for the Nonne-Apert
test I phase positive, suggesting increased spherical Pr.
3. Filter the above mixture, add 0.8 mmol/L acetic acid to a little to make it acidic, and then heat and boil it, 3 minutes there is a white precipitate appeared as Nonne-Apert test tube II phase positive, suggesting increased white Pr.