Source of content: compact guide to molecular biology 5th ed.
Operation method
Enrichment medium preparation experiment
Principle
Prepare as for Extreme Medium, but at high pressure for 25 min (unless otherwise indicated) and without dilution.
Materials and Instruments
Tryptone NaCl NaOH Glycerol Na2HPO4 KNO3 Move 1. In a 2L flask, add the ingredients of the following formula to water and heat and stir until dissolved. For more product details, please visit Aladdin Scientific website.
Glass bottles Flasks
(1) H medium, per liter
10 g tryptone
8 g NaCl
(2) λ broth medium, per liter
10 g tryptone
2.5 g NaCl
(3) NZC broth medium, per liter
10 g NZ Amine A (NZ Amine A)
5 g NaCl
2 g MgCl2 - 6H20
High pressure for 30 min
5 ml 20% casein hydrolysate (casamino acid)
(4) LB medium, per liter
10 g tryptone
5 g yeast extract
5 g NaCl
1 ml 1 mol/L NaOH
(5) Super broth medium, per liter
32 g tryptone
20 g yeast extract
5 g NaCl
5 ml 1 mol/L NaOH
(6) TB (super broth medium), per liter
12 g Bacto Tryptone
24 g Bacto yeast extract
4 ml glycerol
Add water to 900 ml and autoclave, add 100 ml of sterile 0. 17 mol/L KH2PO4 and 0. 72 mol/L K2HPO4.
(7) Tryptic peptone broth medium per liter.
10 g tryptone
5 g NaCl
(8) 2 X TY medium per liter
15 g tryptone
10 g yeast extract
5 g NaCl
(9) TYGPN medium, per liter
20 g tryptone
10 g yeast extract
10 ml 80% glycerol
5 g Na2HPO4
10 g KNO3 2. Pour into a glass vial, loosen the cap and autoclave at 15 lb/in2 for 25 min.
3. Allow the medium to cool to below 50°C (at this temperature the bottle may feel hot when held in the hand but can be gripped), add the other supplements and antibiotics, cool the glass bottle to below 40°C and close the lid tightly.
4. May be stored indefinitely at room temperature.