It is recommended to do FMO-ISO (FMO-ISO) for indicators with low expression or less distinctive clustering, and only one tube is needed. Take IFN-γ as an example, take the “sample of the group expected to express more IFN-γ” as the sample of FMO-ISO, and add other flow antibodies except IFN-γ and IFN-γ isotype control antibody, this tube of sample is called the FMO combined isotype control tube. The rest of the experimental steps were consistent with the experimental group, such as dead-activation and fixation of ruptured membranes.
Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.
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Cite this article
Aladdin Scientific. "How do I set up an FMO combined isotype control?" Aladdin Knowledge Base, updated Sep 13, 2024. https://www.aladdinsci.com/us_en/faqs/how-do-i-set-up-an-fmo-combined-isotype-control-en.html
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