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Laboratory Protocols & SOPs Library

Laemmli Sample Buffer 2X

· Published Jul 27, 2023 · Updated Jun 9, 2025

Recipe

4% SDS
20% glycerol
0.004% bromphenol blue
0.125M Tris-Cl, pH 6.8
10% 2-mercaptoethanol (or DTT) (add immediately before use)

Categories: Laboratory Protocols & SOPs Library

Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.

Cite this article

Aladdin Scientific. "Laemmli Sample Buffer 2X" Aladdin Knowledge Base, updated Jun 9, 2025. https://www.aladdinsci.com/eu_en/faqs/laemmli-sample-buffer-2x-en.html

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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.