Experiments on the analysis of yeasts with subsequent spores
The meiotic products are released from the ascospores, ruptured by sonication, and spread directly on agar plates, and the spore colonies can be screened for th
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Yeast vector and expression product detection experiments
Because of the simplicity and sensitivity of the β-galactosidase assay, yeast genes are frequently labeled with the functional portion of the lacZ gene to indi
Tissue and embryo fixation and embedding experiments
The role of fixation, from the histological point of view its simple definition is to maintain the intrinsic morphology and structure of cells and tissues, so t
Slicing experiments on samples in wax blocks
Paraffin section is the most widely used method in the routine histology technique. Paraffin section is not only used to observe the morphology and structure of
Yeast gene cloning experiment
A general method for cloning yeast genes by complementation using a putative mutation. The mutation is cdc101-1, which is both recessive and temperature sensiti
Yeast cell plasmid isolation experiment
Under non-selective growth conditions, most E. coli/yeast shuttle vectors are lost at a frequency of about 1%, and one plasmid that is not easily isolated and r
Frozen section fixation experiment
This experiment is mainly used for fixing frozen sections.
Handling experiments on pre-coated slides
Slides coated with polylysine (a Thermo product) with durable bioadhesion for attaching frozen and paraffin-embedded sections that can be subsequently centrifug
Yeast cell nucleus preparation experiment
The nucleus (nucleus) is the largest and most important organelle in the cell (the old junior high school textbooks believe that the nucleus is not an organelle