Total RNA or poIy(A) +RNA from Drosophila embryos
RNA Laboratory Techniques Manual (Molecular Cloning - Laboratory Guide Series)
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Determination of RNA-protein dissociation constants by nitrocellulose filter membrane binding assays
The binding of proteins to DNA and RNA can be determined using nitrocellulose membrane binding, which is based on the fact that most proteins bind to nitrocellu
Northwestern screening of protein expression library assay
Northwestern screening of protein expression libraries is performed by constructing IPTG-inducible fusion protein expression libraries (the N-terminus of the fu
RNA footprinting and modification interference analysis experiments
RNA footprinting and modification interference analysis are techniques used to study RNA-protein interactions.The principles of RNA sequencing and its structura
RNaseH cleavage protection experiments for oligonucleotide targeting in RNA- protein complexes
Oligonucleotide-targeted RNase II digestion assay is a method to analyze RNA protein complexes (RNP, ribonucleoprotein particles), both for crude extracts and p
Yeast three-hybrid system for detecting and analyzing RNA-protein interactions
The yeast three-hybrid system can be used to analyze protein-RNA interactions. In the yeast three-hybrid system, RNA-protein interactions lead to transcription
Large-scale preparation of Drosophila RNA
This method allows for the preparation of RNA from 100 Drosophila or approximately 2g of Drosophila embryos.
Liquid-phase screening of RNA-binding proteins in cDNA expression libraries
The method of immobilizing cDNA library-expressed proteins on nitrocellulose or nylon membranes and then screening them with labeled RNA is the most commonly us
Fluorescent RNA random primer-triggered polymerase chain reaction assay
Differential display polymerase chain reaction (PCR) can facilitate the identification of new molecular markers associated with contamination exposure in many s