Removal of ethidium bromide from DNA by ion exchange chromatography
Ethidium bromide in DNA purified by cesium chloride gradient centrifugation can be removed by ion-exchange chromatography, followed by ethanol precipitation to
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Plasmid DNA purification by polyethylene glycol precipitation assay
This method was first devised by Richaed Treisman (ICRF, London, UK) with reference to the work of Lis, who was the first to use polyethylene glycol (PEG) to is
Chemiluminescent Dideoxy DNA Sequencing Assay
Standard double deoxy DNA sequencing can be easily and efficiently detected using non-isobaric labeled chemiluminescence. In the sequencing reaction, biotin-lab
Total DNA quality and digestion assay
The objectives of the experiment are to understand and master the methods of detecting the quality of DNA as well as the methods of DNA quantification; to under
Establishment of randomized overlapping DNA insertion libraries
The following protocol describes the starting steps of birdshot sequencing, from shearing of target DNA to construction of a DNA fragment library with M13 phage
Enzymatic digestion of DNA fragments
Restriction endonucleases are a class of DNA hydrolases that recognize specific nucleotide sequences in double-stranded DNA molecules, and the discovery and app
Removal of small fragments of nucleic acids from plasmid DNA preparations by lithium chloride precipitation
The principle of lithium chloride precipitation for removing small fragments of nucleic acids (both DNA and RNA) from plasmid preparations is based on the fact
Rapid small volume extraction of total plant DNA
DNA molecule is the basic material of molecular biology research, according to different experimental purposes can be taken to different extraction methods to o
Dephosphorylation of plasmid DNA
Removal of the phosphate group at the 5' end prevents self-ligation and cyclization of plasmid DNA. In an in vitro ligation reaction, DNA ligase forms a phospho