λ Alkaline phosphatase treatment assay of phage vector DNA
Removal of the terminal 5'-phosphate groups from the inner ends of the two arms of the λ phage effectively prevents self-association and reduces the back
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Preparation of λ phage DNA by double restriction enzyme cleavage as a cloning vector
Replacement vectors (e.g., λ2001, λDASH, EMBL series, Charon 34, Charon 35, and Charon 40) contain a series of restriction sites that are aligned
Preparation of λ phage DNA that can be cleaved by a single restriction enzyme and used as a cloning vector
In some cases, prepared λ phage DNA can be used for cloning after simple digestion with restriction enzymes. However, this option is only feasible if the
Screening of DNA binding proteins using λ phage libraries
Synthetic double-stranded oligonucleotides can be used to screen cDNA expression libraries constructed in λ phage to identify clones corresponding to spe
Partial cleavage (pre-reaction) of eukaryotic DNA for genomic libraries
Regardless of the base composition and sequence of high-molecular-quality DNA, it can be fragmented in a semi-random fashion by hydrodynamic shearing. However,
Construction of genomic DNA libraries using mucoid vectors
The steps for constructing genomic DNA libraries in λ phage are essentially the same as in mucosal vectors. In both systems, eukaryotic DNA is ligated to vecto
Preparation of single-stranded DNA using phage vectors
Phage plasmids skillfully combine the characteristics of plasmids and filamentous phages. In addition to the basic features, these plasmids are usually high cop
λ Purification of phage arms (by sucrose density gradient centrifugation) experiments
Unlike insertion vectors, replacement vectors contain a filler fragment in the middle of the genome, which must be removed in order to accommodate the foreign D
Large-scale preparation of single- and double-stranded M13 phage DNAs
This protocol is mainly used to prepare large quantities of double-stranded DNA from M13 phage, which is often used as a cloning vector in the laboratory, as we