Agarose gel electrophoresis experiment
Agarose gel electrophoresis can be applied to (1) DNA cutting and recovery, (2) DNA isolation, (3) corroboration of whether DNA is recombinant or not, whether p
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quantitative PCR
Quantitative PCR can be used for: (1) quantification of the amount of PCR starting template by analyzing the PCR end-product or monitoring the PCR process using
Isolation of RNA based on size
RNA samples may be denatured by treatment with formamide and by electrophoretic separation on gels containing formaldehyde, a method modified from Lehrachetal.
Automated Laser Fluorescence DNA Sequencing
At present, the application of automated laser fluorescence DNA sequencing technology (also known as first generation sequencing technology) has been very commo
In-situ notch panning technique
The in situ nick translation technique is also known as in situ nick translation. It was first used to study DNA replication in vitro, and has since been extend
Microbial Genome DNA Extraction
The yeast is first lysed using a solution containing leaving salts to ensure complete denaturation of the macromolecules. Afterwards, ethanol is added and the D
Polyclonal antibody purification assay
The octanoic acid extraction method can be used to isolate and extract Ig from human, rabbit and mouse serum and McAb from hybridoma-induced ascites and culture
Cross-protection neutralization experiments
When an animal is infected by a virus, specific neutralizing antibodies are produced in the body and bind specifically to the corresponding virus particles, thu
Speckled gold immunofiltration assay (DIGFA) assay
The spotted gold immunofiltration assay (DIGFA) is available as a clinical test.