In vivo activation of macrophages
Macrophages are important natural immune cells in vivo, and in vitro detection of their function does not fully reflect their in vivo behavior, while detection
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Isolation of peripheral blood single nucleated cells
Immune cell isolation techniques are developing in the direction of more and more "fine", immune cell subpopulation and even micro-immune cell isolation and ide
Flow cytometric analysis of fluorescence resonance energy transfer
The study of various cellular life processes has been made easier by the detection of intermolecular interactions in vivo and in vitro through a variety of meth
Mammalian protein-protein interaction traps based on fluorescence-activated cell sorting technology
The Mammalian Protein-Protein Interaction Trap System (MAPPIT) is a two-hybrid system designed based on the understanding of the signaling mechanism of type I c
Combined application of flow cytometry and gene chip methods to detect transcriptional profiles
The two technologies, flow cytometry and gene expression profiling, share many common features: they involve complex, highly sophisticated equipment; they can p
Detection and enrichment of hematopoietic stem cells by marginal group cell phenotyping
Detection and enrichment of hematopoietic stem cells by marginal group cell phenotyping is based on the isolation of hematopoietic stem cells from mouse bone ma
Flow cytometry analysis of cytokines
Cytokineflow cytometry (CFC) is the collective term for flow cytometry that applies anti-cytokine antibodies to analyze cytokines that are markers of cell activ
Multi-parameter analysis of apoptosis using flow and image cytometry
Apoptosis regulates the dynamic balance of cells, and the multiparametric nature of flow cytometry allows for the simultaneous determination of several apoptoti
Characterization of hematopoietic stem cells
Hoechst 33342 and Rhodamine123 Staining for Hematopoietic Stem Cell Characterization Given that the in vitro in vivo dyes Rhodamine123 (Rh123) and Hoechst 33342