Detection of apoptosis in tumor cells by DNA degradation and fracture assay
DNA degradation fracture assay for detection of apoptosis in tumor cells can be used to (1) detect apoptosis in tumor cells and (2) detect the effect of drug tr
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Calcein release assay to detect cytotoxic T-lymphocyte activity assay
The source of this experiment is the official website of the Fourth Military Medical University
125I-UdR release assay to determine NK cell activity
Natural killer (NK) cells are involved in the first line of the body's immune defense and are mainly used in (1) anti-tumor and anti-virus (2) immunomodulation
Preparation of medium with 1× stock solution
Check the design of the formulation: if the formulation is complete, it can be used directly after the addition of serum; if the formulation is incomplete (e.g.
Lymphocyte proliferation test
Lymphocyte proliferation test, usually under the stimulation of antigenic substances such as mitogens and other antigenic substances, proliferation, differentia
DAPI staining
Cell staining with DAPI
NK cell activity assay: LDH method
This experiment was mainly used to determine NK cell activity.
DNA synthesis assay with 3H-TdR doping assay
Cells were labeled with 3H -TdR and DNA was extracted to determine radioactivity levels by scintigraphy Source : Animal Cell Culture : A Guide to Basic Techniqu
T lymphocyte transformation assay
When T cells are stimulated by non-specific mitogens (e.g. PHA, ConA) during in vitro culture, they may be transformed into lymphoblastoid cells with increased