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Laboratory Protocols & SOPs Library

3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.

3228 articles
Laboratory Protocols & SOPs Library

SSPE 20X

SSPE 20X

2026-07-24 02:21:13 Updated
Laboratory Protocols & SOPs Library

Mallory Phosphotungstic Acid Hematoxylin Method for Staining Striated Muscle Cross-Striations, Fibrin, and Neuroglial Fibers: Experimental Protocol

This protocol is used to establish a routine staining procedure for the Mallory phosphotungstic acid hematoxylin method. It is suitable for displaying striated muscle cross-striations, fibrin, and neuroglial fibers in paraffin-embedded tissue sections. It can also assist in observing tissue ...

2026-07-22 07:21:49 Updated
Laboratory Protocols & SOPs Library

Puchtler Tannic Acid–Azophloxine Method for Muscle Fiber Staining

This protocol establishes a routine operating procedure for muscle fiber staining using the Puchtler tannic acid–azophloxine method. It is suitable for differentiating muscle fibers and collagen fibers in paraffin-embedded tissue sections. It can be used for morphological studies of smooth ...

2026-07-22 06:45:53 Updated
Laboratory Protocols & SOPs Library

Bennhold Congo Red Staining Protocol for Amyloid Substances

This protocol is intended to establish a routine staining workflow for the Bennhold Congo red method. It is applicable to the demonstration and morphological observation of amyloid substances in paraffin-embedded tissue sections and can be used for histopathological research, evaluation of ...

2026-07-22 05:43:19 Updated
Laboratory Protocols & SOPs Library

Protocol for Osteoblast Mineralization on β-Tricalcium Phosphate Scaffolds and Quantitative Alizarin Red S Assay

This protocol is intended to establish a workflow for mineralization induction of primary osteoblasts on β-tricalcium phosphate (β-TCP) discs and quantitative detection using Alizarin Red S (ARS) staining. It is applicable to the in vitro evaluation of mineralization capacity in bone ...

2026-07-22 05:22:50 Updated
Laboratory Protocols & SOPs Library

Bacterial Capsule Negative Staining Protocol

This protocol establishes a routine operating procedure for bacterial capsule negative staining. It is suitable for morphological observation and preliminary identification of encapsulated bacteria, and can be used in routine microbiology laboratory testing, teaching demonstrations, and strain ...

2026-07-22 02:43:30 Updated
Laboratory Protocols & SOPs Library

Phosphotungstic Acid Negative Staining Method for TEM Sample Preparation

This protocol establishes a routine operating procedure for the phosphotungstic acid negative staining method. It is suitable for negative staining observation of viruses, bacteriophages, exosomes, liposomes, protein complexes, ribosomes, nucleic acid-protein complexes, bacterial flagella/pili, ...

2026-07-22 02:24:08 Updated
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