Experimental determination of total serum proteins by bis-urea method
All compounds containing two formamide groups (-CONH2-) in the molecule can interact with alkaline copper solution to form a purplish-red compound, this reactio
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Plasma fibrinogen assay
Through this experiment, trainees can master the principle and method of plasma fibrinogen determination and the preparation of reagents. This experiment is fro
Serum total bilirubin and conjugated bilirubin determination assay
Bound bilirubin in serum can react directly with diazo reagent to produce azo bilirubin, under the same conditions, free bilirubin must have an accelerator, so
Experiment for the determination of serum chloride by the colorimetric method of mercury thioglycolate
Chloride in the specimen interacts with mercury thiocyanate to produce mercury chloride that is difficult to dissociate and releases a considerable amount of th
Colorimetric determination of total serum calcium by o-cresolphthalein complexone assay
The near-o-cresolphthalein complexone colorimetric assay for the determination of total serum calcium test can be used clinically to measure serum calcium ion l
Serum triglyceride determination by acetone acetate assay experiment
This method uses isopropanol to extract serum TG, alumina adsorption of phospholipids and free glycerol saponification with potassium hydroxide, sodium periodat
Serum L-T-glutamate transpeptidase (GGT) assay experiment
This method uses L-T-glutamic acid α-naphthylamine as a substrate, and under GGT catalysis, the T-glutamic acid group is transferred to bis-glycopeptide fracti
Serum total cholesterol assay
Isopropanol was used, and bilirubin was adsorbed with alumina during extraction equal to the interferents (phospholipids and glycerol were also adsorbed, and th
Direct method of serum potassium-sodium flame photometric analysis experiment
Flame photometric analysis, is a kind of emission spectrometry, the solution to be measured by compressed air atomization in the combustible gas mixture after c