Recombinant Human C8orf76 Protein: Overview & Research Use

Recombinant Human C8orf76 Protein is a research-grade recombinant protein derived from the human chromosome 8 open reading frame 76 gene, produced for use in biochemical characterization, protein interaction studies, and functional assays.

Gene Name

C8orf76 (Chromosome 8 Open Reading Frame 76)

Species Origin

Homo sapiens (Human)

Product SKU

rp376078

Reference Catalog

absea#PP-9698

Protein Type

Recombinant protein produced via heterologous expression system

Intended Use

Research use only (RUO); not for diagnostic or therapeutic purposes

What Is C8orf76? Molecular Background

C8orf76, or Chromosome 8 Open Reading Frame 76, is a protein-coding gene located on human chromosome 8. Like many open reading frame (ORF) proteins, C8orf76 was initially identified through genome sequencing efforts and its precise biological function is still under active investigation by the scientific community. Proteins in the ORF category often represent understudied or novel proteins whose functional roles are being elucidated through proteomic, transcriptomic, and functional genomic approaches.

Recombinant production of C8orf76 enables researchers to study this protein in isolation, characterize its biochemical properties, identify interaction partners, and investigate its potential involvement in cellular processes. Understanding such proteins contributes to a broader comprehension of the human proteome and may reveal new biological pathways or disease associations.

Expression, Production & Quality Specifications

Recombinant Human C8orf76 Protein (SKU: rp376078) is produced using standard recombinant protein expression technologies. Recombinant proteins of human origin are commonly expressed in bacterial (E. coli), insect (baculovirus), mammalian, or yeast-based expression systems, each offering distinct advantages regarding protein folding, post-translational modifications, and yield.

For research applications requiring native-like folding and potential post-translational modifications, mammalian or insect cell expression systems are typically preferred. Bacterial expression systems offer high yield and cost efficiency but may require refolding steps for complex eukaryotic proteins. Researchers should consult the product datasheet (referenced as absea#PP-9698) for confirmed expression host, purification method, purity grade, molecular weight, formulation, and storage recommendations specific to this lot.

Quality control testing for recombinant proteins typically includes SDS-PAGE for purity assessment, endotoxin testing (LAL assay), and bioactivity assays where applicable. Always verify the certificate of analysis (CoA) upon receipt to confirm that the product meets the stated specifications before use in critical experiments.

Research Applications

Recombinant Human C8orf76 Protein is suitable for a range of in vitro research applications. Common uses for recombinant proteins of this type include:

• Protein–Protein Interaction Studies: Used as bait or prey in co-immunoprecipitation (co-IP), pull-down assays, or yeast two-hybrid validation experiments to map interaction networks.
• Antibody Production and Validation: Serving as an immunogen for raising polyclonal or monoclonal antibodies, or as a standard antigen for ELISA and Western blot validation.
• Biochemical Characterization: Enabling enzymatic assays, structural studies, and binding kinetics measurements (e.g., surface plasmon resonance, biolayer interferometry) if the protein possesses enzymatic or binding activity.
• Cell-Based Assays: Investigating the effect of exogenous C8orf76 protein on cellular phenotypes when applied to cell culture systems.
• Functional Genomics Support: Complementing CRISPR knockout or RNAi knockdown studies by providing recombinant rescue protein for functional restoration experiments.

All applications should be conducted under conditions validated for this specific recombinant protein. Refer to the product datasheet for recommended working concentrations and validated application notes.

Handling, Storage & Reconstitution Guidelines

Proper handling and storage of recombinant proteins are essential to maintain activity and integrity. General best-practice guidelines for recombinant proteins include:

• Storage Temperature: Most recombinant proteins are stored at −20 °C or −80 °C for long-term stability. Avoid repeated freeze–thaw cycles, which can cause protein aggregation and loss of activity. Aliquot upon first thaw.
• Reconstitution: If supplied lyophilized, reconstitute in the buffer specified on the datasheet (typically sterile PBS or a carrier-protein-containing buffer). Allow the protein to equilibrate gently; do not vortex vigorously.
• Working Conditions: Keep reconstituted protein on ice during experiments. For short-term storage (up to 1 week), 4 °C with a carrier protein such as 0.1% BSA may be acceptable.
• Sterility: For cell-based assays, use sterile technique and consider filtering through a 0.22 µm membrane if endotoxin contamination is a concern.

Always consult the product-specific datasheet (absea#PP-9698) for formulation details, recommended reconstitution volume, and validated storage conditions, as these may differ from general guidelines.

Ordering Information & Technical Support

Recombinant Human C8orf76 Protein is available from Aladdin Scientific under SKU rp376078. This product is referenced under catalog number absea#PP-9698.

When placing an order, procurement teams and lab managers should confirm:

• Required quantity and available pack sizes
• Desired purity grade and expression host (consult datasheet)
• Shipping and cold-chain requirements (dry ice shipment for frozen proteins)
• Current lot availability and lead time

For technical questions regarding experimental design, application optimization, or product compatibility, please contact Aladdin Scientific's technical support team. Custom recombinant protein services, including tag variants or large-scale production, may also be available upon inquiry.

Note: This product is for research use only (RUO) and is not intended for human or veterinary diagnostic or therapeutic applications.

Frequently asked questions

What is Recombinant Human C8orf76 Protein used for in research?

Recombinant Human C8orf76 Protein is primarily used for in vitro research applications including protein–protein interaction studies, antibody production and validation, biochemical characterization, and cell-based functional assays. It supports efforts to elucidate the biological role of the C8orf76 gene product within the human proteome.

How should Recombinant Human C8orf76 Protein be stored to preserve activity?

Recombinant proteins are generally stored at −20 °C or −80 °C for long-term stability. To avoid loss of activity, minimize repeated freeze–thaw cycles by aliquoting the protein upon first thaw. Always refer to the product-specific datasheet (absea#PP-9698) for the confirmed formulation and validated storage conditions for this product.

Is Recombinant Human C8orf76 Protein suitable for diagnostic or therapeutic use?

No. Recombinant Human C8orf76 Protein (SKU: rp376078) is designated for research use only (RUO). It is not approved or validated for use in human or veterinary clinical diagnostics, therapeutic applications, or any in vivo procedures.

What expression system is used to produce Recombinant Human C8orf76 Protein?

The specific expression system used for Recombinant Human C8orf76 Protein (rp376078) is detailed in the product datasheet referenced as absea#PP-9698. Common systems for human recombinant proteins include E. coli, insect, mammalian, and yeast expression platforms. Please consult the datasheet or contact Aladdin Scientific technical support for confirmed expression host information.

How do I place an order for Recombinant Human C8orf76 Protein?

Recombinant Human C8orf76 Protein can be ordered from Aladdin Scientific using SKU rp376078. Contact the Aladdin Scientific sales or procurement team to confirm pack size availability, current lot status, pricing, and cold-chain shipping arrangements prior to placing your order.

References

1. Aladdin Scientific. Recombinant Human C8orf76 Protein, SKU rp376078. Product Datasheet absea#PP-9698.
2. UniProt Consortium. UniProtKB entry for human C8orf76 protein. UniProt database.
3. NCBI Gene. C8orf76 chromosome 8 open reading frame 76 [Homo sapiens]. National Center for Biotechnology Information.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.