Recombinant Human CFAP70 Protein: Key Facts & FAQs

Recombinant Human CFAP70 Protein is a research-grade recombinant protein associated with cilia and flagella assembly and function, available from Aladdin Scientific (SKU: rp373950) to support studies in ciliopathy research, cell biology, and related fields.

Product Name

Recombinant Human CFAP70 Protein

SKU

rp373950

Gene Name

CFAP70 (Cilia and Flagella Associated Protein 70)

Species Origin

Human (Homo sapiens)

Protein Type

Recombinant protein, research grade

Supplier

Aladdin Scientific

What Is CFAP70 and Why Is It Relevant to Research?

CFAP70, or Cilia and Flagella Associated Protein 70, is a protein encoded by the CFAP70 gene in humans. It plays a role in the assembly and structural integrity of cilia and flagella—hair-like organelles critical for cell motility, fluid movement, and signal transduction in a wide range of cell types.

Research into CFAP70 is relevant to the study of ciliopathies, a broad class of genetic disorders arising from defective cilia function. These conditions include primary ciliary dyskinesia (PCD), Bardet-Biedl syndrome, and certain forms of infertility associated with sperm flagella defects. The Recombinant Human CFAP70 Protein offered by Aladdin Scientific (SKU: rp373950) enables researchers to investigate the biochemical properties and interaction partners of this protein in vitro.

Product Specifications and Quality

The Recombinant Human CFAP70 Protein (SKU: rp373950) is produced for research use. As with all recombinant proteins in the Aladdin Scientific catalog, it is intended to provide consistent lot-to-lot quality to support reproducible experimental outcomes.

Researchers should consult the official product datasheet available on the Aladdin Scientific website for confirmed specifications, including expression host, tag information, purity, formulation, and recommended storage conditions. Specific numerical values for purity, concentration, and molecular weight should be verified against the current datasheet, as these parameters are subject to lot variation and periodic updates.

Handling, Storage, and Reconstitution Guidelines

Proper handling and storage of recombinant proteins are critical to maintaining activity and avoiding degradation. General best practices for research-grade recombinant proteins include:

• Storage temperature: Most recombinant proteins should be stored at −20 °C or −80 °C upon receipt to preserve stability. Avoid repeated freeze-thaw cycles by aliquoting the protein into single-use volumes.
• Reconstitution: If supplied lyophilized, reconstitute in the buffer recommended in the product datasheet. Allow the vial to equilibrate to room temperature before opening, then add the diluent slowly and mix gently—do not vortex.
• Working conditions: Keep the protein on ice during experimental setup and minimize exposure to room temperature.
• Carrier protein: Adding a carrier protein such as BSA (0.1–1% final concentration) may improve stability during dilution steps.

Always refer to the Aladdin Scientific product datasheet for product-specific storage and reconstitution instructions, as these supersede general guidelines.

Ordering, Shipping, and Returns

Recombinant Human CFAP70 Protein (SKU: rp373950) can be ordered directly through the Aladdin Scientific website or by contacting the Aladdin Scientific sales team. The following practical information applies to this product:

• Ordering: Provide the SKU rp373950 when placing your order to ensure accurate fulfillment. Bulk or custom quantities may be available upon request—contact Aladdin Scientific for pricing and lead times.
• Shipping: Recombinant proteins are typically shipped on dry ice to maintain the cold chain. Confirm shipping conditions and delivery timelines with Aladdin Scientific at the time of ordering, as these may vary by region and product format.
• Returns and refunds: Please review Aladdin Scientific's current returns and refund policy on the official website. Biological reagents are subject to specific return conditions; contact customer service promptly upon receipt if there are any quality concerns.

Recommended Applications and Experimental Considerations

Recombinant Human CFAP70 Protein is intended for research use only and is not approved for diagnostic or therapeutic applications. Typical research applications for recombinant proteins of this class include:

• Protein–protein interaction studies: Pull-down assays, co-immunoprecipitation, and surface plasmon resonance (SPR) to identify binding partners of CFAP70.
• Antibody validation: Use as a positive control antigen for anti-CFAP70 antibody testing by ELISA or Western blot.
• Biochemical assays: Structural or functional characterization of CFAP70 in reconstituted in vitro systems.
• Cell biology research: Studies on cilia assembly pathways and ciliopathy mechanisms.

Confirm suitability for your specific assay format with the product datasheet and, where necessary, perform pilot experiments to optimize protein concentration and assay conditions before committing to full-scale experiments.

Frequently asked questions

What is the recommended storage condition for Recombinant Human CFAP70 Protein (rp373950)?

Recombinant proteins should generally be stored at −20 °C or −80 °C to preserve stability and activity. To prevent loss of activity through repeated freeze-thaw cycles, aliquot the protein into single-use volumes immediately upon receipt. Always refer to the specific storage instructions provided in the Aladdin Scientific product datasheet for rp373950, as these take precedence.

What research applications is Recombinant Human CFAP70 Protein suitable for?

Recombinant Human CFAP70 Protein is suitable for a range of in vitro research applications including protein–protein interaction studies (such as pull-down and co-immunoprecipitation assays), antibody validation as a positive control antigen, biochemical characterization, and cilia biology research. It is intended for research use only and is not approved for diagnostic or therapeutic purposes. Please confirm suitability for your specific application with the product datasheet.

How should I reconstitute lyophilized Recombinant Human CFAP70 Protein?

If the product is supplied in lyophilized form, allow the vial to equilibrate to room temperature before opening to minimize condensation. Reconstitute with the buffer type and volume specified in the product datasheet, adding the diluent slowly and mixing gently by pipetting or rolling—do not vortex, as this can denature the protein. Allow a few minutes for complete dissolution before use or aliquoting.

Can Recombinant Human CFAP70 Protein be used as an antibody validation standard?

Yes, recombinant proteins such as CFAP70 are commonly used as positive control antigens to validate anti-CFAP70 antibodies in assays such as ELISA and Western blot. Using a well-characterized recombinant protein helps confirm antibody specificity and sensitivity. Check the product datasheet to confirm purity and formulation details relevant to your specific validation workflow.

How do I place an order for Recombinant Human CFAP70 Protein from Aladdin Scientific?

You can order Recombinant Human CFAP70 Protein by searching for SKU rp373950 on the Aladdin Scientific website or by contacting the Aladdin Scientific sales team directly. For bulk quantities or custom requirements, please reach out to customer service for pricing and availability. Ensure you reference the SKU rp373950 when placing your order to avoid fulfillment errors.

References

1. Aladdin Scientific product page for Recombinant Human CFAP70 Protein (SKU: rp373950). Aladdin Scientific.
2. Mitchison HM, Valente EM. Motile and non-motile cilia in human pathology: from function to phenotypes. Journal of Pathology. 2017;241(2):294-309. doi:10.1002/path.4843
3. Reiter JF, Leroux MR. Genes and molecular pathways underpinning ciliopathies. Nature Reviews Molecular Cell Biology. 2017;18(9):533-547. doi:10.1038/nrm.2017.60

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.